Towards Dissecting the Pathogenesis of Retinoid-Induced Hair Loss: All-Trans Retinoic Acid Induces Premature Hair Follicle Regression (Catagen) by Upregulation.

Slides:

Advertisements

Similar presentations

Vladimir A. Botchkarev, Jiro Kishimoto

Advertisements

Histologic Study of the Regeneration Process of Human Hair Follicles Grafted onto SCID Mice after Bulb Amputation Tsuyoshi Hashimoto, Takashi Kazama,

NF-κB Activity Is Required for Anagen Maintenance in Human Hair Follicles In Vitro Jennifer E. Kloepper, Nancy Ernst, Karsten Krieger, Enikő Bodó, Tamás.

Profiling the Response of Human Hair Follicles to Ultraviolet Radiation Zhongfa Lu, Tobias W. Fischer, Sybille Hasse, Koji Sugawara, York Kamenisch, Sven.

Expression of Frizzled Genes in Developing and Postnatal Hair Follicles Seshamma T. Reddy, Thomas Andl, Min-Min Lu, Edward E. Morrisey, Sarah E. Millar,

Liat Samuelov, Eli Sprecher, Koji Sugawara, Suman K. Singh, Desmond J

Corticotropin-Releasing Hormone Stimulates the In Situ Generation of Mast Cells from Precursors in the Human Hair Follicle Mesenchyme Natsuho Ito, Koji.

A Comprehensive Guide for the Accurate Classification of Murine Hair Follicles in Distinct Hair Cycle Stages Sven Müller-Röver, Kerstin Foitzik, Ralf.

Expression of Frizzled Genes in Developing and Postnatal Hair Follicles Seshamma T. Reddy, Thomas Andl, Min-Min Lu, Edward E. Morrisey, Sarah E. Millar,

Expression patterns of the glial cell line–derived neurotrophic factor, neurturin, their cognate receptors GFRα-1, GFRα-2, and a common signal transduction.

A New Strategy for Modulating Chemotherapy-Induced Alopecia, Using PTH/PTHrP Receptor Agonist and Antagonist Eva M.J. Peters, Kerstin Foitzik, Ralf Paus,

Andrey A. Panteleyev, Pamela J. Mitchell, Ralf Paus, Angela M

István Borbíró, Erika Lisztes, Balázs I

Nathan J. Hawkshaw, Iain S. Haslam, David M

Kit Is Expressed by Epithelial Cells In Vivo

Kai Kretzschmar, Denny L. Cottle, Pawel J. Schweiger, Fiona M. Watt

A Meeting of Two Chronobiological Systems: Circadian Proteins Period1 and BMAL1 Modulate the Human Hair Cycle Clock Yusur Al-Nuaimi, Jonathan A. Hardman,

The Thyroid Hormone Analogue KB2115 (Eprotirome) Prolongs Human Hair Growth (Anagen) Ex Vivo Attila Oláh, Jennifer Gherardini, Marta Bertolini, Jérémy.

A. Godwin Diamond, Ryan M. Gonterman, Aileen L

Profiling mRNA of the Graying Human Hair Follicle Constitutes a Promising State-of-the- Art Tool to Assess Its Aging: An Exemplary Report Eva M.J. Peters,

Thymic Peptides Differentially Modulate Human Hair Follicle Growth

P-Cadherin Regulates Human Hair Growth and Cycling via Canonical Wnt Signaling and Transforming Growth Factor-β2 Liat Samuelov, Eli Sprecher, Daisuke.

A technique for more precise distinction between catagen and telogen human hair follicles ex vivo Irene Hernandez, PhD, Majid Alam, PhD, Christopher.

James M. Waters, Jessica E. Lindo, Ruth M. Arkell, Allison J. Cowin

Wanglong Qiu, Xiaojun Li, Hongyan Tang, Alicia S. Huang, Andrey A

Desmond J. Tobin Journal of Investigative Dermatology

Yasuyuki Amoh, Lingna Li, Kensei Katsuoka, Robert M. Hoffman

The mRNA for Protease Nexin-1 is Expressed in Human Dermal Papilla Cells and its Level is Affected by Androgen Tadashige Sonoda, Yuji Asada, Sotaro Kurata,

NF-κB Participates in Mouse Hair Cycle Control and Plays Distinct Roles in the Various Pelage Hair Follicle Types Karsten Krieger, Sarah E. Millar, Nadine.

Roles of GasderminA3 in Catagen–Telogen Transition During Hair Cycling

Active Hair Growth (Anagen) is Associated with Angiogenesis

Fas and c-kit are Involved in the Control of Hair Follicle Melanocyte Apoptosis and Migration in Chemotherapy-Induced Hair Loss Andrei A. Sharov, Guang-Zhi.

Integrin β6-Deficient Mice Show Enhanced Keratinocyte Proliferation and Retarded Hair Follicle Regression after Depilation Yanshuang Xie, Kevin J. McElwee,

Topical Estrogen Accelerates Hair Regrowth in Mice After Chemotherapy-Induced Alopecia by Favoring the Dystrophic Catagen Response Pathway to Damage

Localization of Plasminogen Activator Inhibitor Type 2 (PAI-2) in Hair and Nail: Implications for Terminal Differentiation Robert M. Lavker, Barbara.

Adenosine Stimulates Fibroblast Growth Factor-7 Gene Expression Via Adenosine A2b Receptor Signaling in Dermal Papilla Cells Masato Iino, Ritsuko Ehama,

Dickkopf 1 Promotes Regression of Hair Follicles

Natalia V. Botchkareva, Vladimir A. Botchkarev, Barbara A. Gilchrest

Complex Changes in the Apoptotic and Cell Differentiation Programs during Initiation of the Hair Follicle Response to Chemotherapy Tatyana Y. Sharova,

NF-κB Activity Is Required for Anagen Maintenance in Human Hair Follicles In Vitro Jennifer E. Kloepper, Nancy Ernst, Karsten Krieger, Enikő Bodó, Tamás.

Thyrotropin-Releasing Hormone Selectively Stimulates Human Hair Follicle Pigmentation Erzsébet Gáspár, Kim T. Nguyen-Thi, Celine Hardenbicker, Stephan.

Mitochondrial Function in Murine Skin Epithelium Is Crucial for Hair Follicle Morphogenesis and Epithelial–Mesenchymal Interactions Jennifer E. Kloepper,

Epithelial Stem Cells: A Folliculocentric View

Slc1a3-CreER as a Targeting Tool for the K6+ Epithelial Stem Cell Niche and its Precursors during Mouse Hair Follicle Cycle Aiko Sada, Prachi Jain, Sherry.

Expression of Calcium-Binding S100 Proteins A4 and A6 in Regions of the Epithelial Sac Associated with the Onset of Hair Follicle Regeneration Mayumi.

Modulation of Hair Growth with Small Molecule Agonists of the Hedgehog Signaling Pathway Rudolph D. Paladini, Jacqueline Saleh, Changgeng Qian, Guang-Xin.

Molecular Mechanisms Regulating Hair Follicle Development

Dissociated Human Dermal Papilla Cells Induce Hair Follicle Neogenesis in Grafted Dermal–Epidermal Composites Rajesh L. Thangapazham, Peter Klover, Ji-an.

Advanced Inhibition of Undesired Human Hair Growth by PPARγ Modulation? Yuval Ramot, Arianna Mastrofrancesco, Erika Herczeg-Lisztes, Tamás Bíró, Mauro.

Andreya Sharov, Desmond J. Tobin, Tatyana Y

Distinct Roles for Nerve Growth Factor and Brain-Derived Neurotrophic Factor in Controlling the Rate of Hair Follicle Morphogenesis Natalia V. Botchkareva,

Vladimir A. Botchkarev, Natalia V. Botchkareva, Kathryn M

Identification of Differentially Expressed Genes During a Wool Follicle Growth Cycle Induced by Prolactin Nicholas W. Rufaut, Allan J. Pearson, Allan.

Loss of γδ T Cells Results in Hair Cycling Defects

Delineating Immune-Mediated Mechanisms Underlying Hair Follicle Destruction in the Mouse Mutant Defolliculated Fiona Ruge, Aikaterini Glavini, Awen M.

Substance P as an Immunomodulatory Neuropeptide in a Mouse Model for Autoimmune Hair Loss (Alopecia Areata) Frank Siebenhaar, Andrey A. Sharov, Eva M.J.

Dual-Mode Regulation of Hair Growth Cycle by Two Fgf-5 Gene Products

Dihydrotestosterone-Inducible IL-6 Inhibits Elongation of Human Hair Shafts by Suppressing Matrix Cell Proliferation and Promotes Regression of Hair Follicles.

Distinct Roles for Nerve Growth Factor and Brain-Derived Neurotrophic Factor in Controlling the Rate of Hair Follicle Morphogenesis Natalia V. Botchkareva,

A Potential Suppressor of TGF-β Delays Catagen Progression in Hair Follicles Yumiko Tsuji, Sumiko Denda, Tsutomu Soma, Laurel Raftery, Takashi Momoi,

Thrombospondin-1 Plays a Critical Role in the Induction of Hair Follicle Involution and Vascular Regression During the Catagen Phase Kiichiro Yano, Michael.

MHC Class I Expression in Murine Skin: Developmentally Controlled and Strikingly Restricted Intraepithelial Expression During Hair Follicle Morphogenesis.

Vladimir A. Botchkarev, Jiro Kishimoto

The WNT Signalling Modulator, Wise, is Expressed in an Interaction-Dependent Manner During Hair-Follicle Cycling Ryan F.L. O'Shaughnessy, Weeteck Yeo,

A Human Folliculoid Microsphere Assay for Exploring Epithelial– Mesenchymal Interactions in the Human Hair Follicle Blanka Havlickova, Tamás Bíró, Alessandra.

On the Regulation of Hair Keratin Expression: Lessons from Studies in Pilomatricomas Bernard Cribier, Bernard Peltre, Edouard Grosshans, Lutz Langbein,

Yasushi Hanakawa, Hong Li, Chenyan Lin, John R

Liren Tang, Shabnam Madani, Harvey Lui, Jerry Shapiro

Tsutomu Soma, Cord E. Dohrmann, Toshihiko Hibino, Laurel A. Raftery

Mind the (Gender) Gap: Does Prolactin Exert Gender and/or Site-Specific Effects on the Human Hair Follicle? Ewan A. Langan, Yuval Ramot, Vincent Goffin,

Presentation transcript:

Towards Dissecting the Pathogenesis of Retinoid-Induced Hair Loss: All-Trans Retinoic Acid Induces Premature Hair Follicle Regression (Catagen) by Upregulation of Transforming Growth Factor-β2 in the Dermal Papilla Kerstin Foitzik, Tanja Spexard, Motonobu Nakamura, Ursula Halsner, Ralf Paus Journal of Investigative Dermatology Volume 124, Issue 6, Pages 1119-1126 (June 2005) DOI: 10.1111/j.0022-202X.2005.23686.x Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Human hair follicles after 6 d of organ culture. (A–F) Macroscopic, (a–f) H&E staining. (A+a) Control (anagen VI), (B+b) all-trans retinoic acid (ATRA) 10−10 M (early catagen), arrow: volume of the hair matrix compartment has diminished, (C+c) ATRA 10−8 M (mid-catagen), (D+d) ATRA 10−6 M (late catagen), (E+e) transforming growth factor (TGF)-β2 10 ng per mL (late catagen), (F+f) ATRA 10−8 supplemented with anti-TGF-β1–3, antibody (anti-TGF-β) (anagen VI). M Star: condensed dermal papilla, (arrow, c, d) pigmented lower end of the hair shaft has moved upwards, (c) cessation of pigmentation, arrowhead: epithelial strand in late catagen Journal of Investigative Dermatology 2005 124, 1119-1126DOI: (10.1111/j.0022-202X.2005.23686.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Hair growth and hair cycle slopes after treatment with ATRA and TGF-β2. (A) Graph shows length of hair shafts of all-trans retinoic acid (10−6, 10−8 M) and transforming growth factor (TGF)-β2-treated hair follicles (HF) compared with control (n=30 HF per group) at days 0, 2, 4, and 6 in culture. Statistical significance was calculated using the Mann–Whitney U test. *p<0.05, **p<0.001. (B) Corresponding hair-cycle score (HCS). Calculation of the HCS: all HF of each group were staged and each stage of the hair cycle has been scored as follows: anagen=100, early catagen=200, late catagen=300. The HCS indicates the mean of the stages of all HF per group. *p<0.01 Journal of Investigative Dermatology 2005 124, 1119-1126DOI: (10.1111/j.0022-202X.2005.23686.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Total number of Ki67- and TdT-mediated dUTP nick end-labeling (TUNEL)-positive cells after 6 d in culture. All-trans retinoic acid-treated hair follicles show an upregulation of apoptotic cells, whereas proliferation is significantly reduced. Statistical significance was calculated using the Mann–Whitney U test. **p<0.01 Journal of Investigative Dermatology 2005 124, 1119-1126DOI: (10.1111/j.0022-202X.2005.23686.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Down regulation of proliferation and upregulation of TGF-β2 hair scripts in the dermol papilla after ATRA treatment. (A) Left panel: Human anagen VI hair follicle (HF) after 6 d in culture. Red fluorescence shows Ki67-positive cells and green fluorescence labels TdT-mediated dUTP nick end-labeling (TUNEL)-positive cells (arrows). Right panel: all-trans retinoic acid (ATRA) 10−8 M treated anagen VI HF after 6 d in culture. Ki67-positive cells in the hair bulb are downregulated. TUNEL-positive cells (arrows) increased compared with control. (B) Transforming growth factor (TGF)-β2 (green) expression in human HF after 4 d in culture without and with ATRA treatment. Left: control; right: ATRA 10−8 M treated anagen HF. Note TGF-β2 upregulation in the dermal papilla of ATRA-treated HF. Lower pictures show magnification of upper dermal papilla sections. IRS, inner root sheath; ORS, outer root sheath; DP, dermal papilla Journal of Investigative Dermatology 2005 124, 1119-1126DOI: (10.1111/j.0022-202X.2005.23686.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Transforming growth factor (TGF)-β2 light-cycler RT-PCR. RNA was separately extracted from the distal part (hair shaft) and the proximal part including dermal papilla (hair bulb) of each hair follicle (HF). Upper graph shows GAPDH and lower graph shows TGF-β2 relative mRNA expression. ***p<0.0006 Journal of Investigative Dermatology 2005 124, 1119-1126DOI: (10.1111/j.0022-202X.2005.23686.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions