Protein delivery: DNA nanostructures and cell-surface targeting Harvard iGEM August 27, 2006

The Machine Goal: Future modularized drug delivery target cell protein

DNA Nanostructures Overview Can design DNA double helices to stick together and form interesting structures. Dr. Ned Seeman, NYU Dr. William Shih, Harvard Paul Rothemund, Caltech A 1.7-kilobase single-stranded DNA that folds into a nanoscale octahedron WILLIAM M. SHIH, JOEL D. QUISPE & GERALD F. JOYCE
Nature 427, 618ﾐ621 (2004); doi:10.1038/nature02307

Motivation: Why DNA? Fascinating area of research The power of DNA Watson-Crick base pairing is enormously strong Self-assembly Highly programmable, designable Specificity - targeting to cells

Design Details

Design Details: Scaffolded Oragami

Design Details: Scaffolded Oragami

Design Details: Scaffolded Oragami

Design Details: Positional Control

Design Details: Positional Control

Design Details: Positional Control

Design Details: Positional Control

Design Details: Positional Control

Design Details: Positional Control

Progress Built a number of barrel designs Exciting EM Images Purifying Nanostructures (nearly there after 1 month of trials)

Exciting EM Images

Exciting EM Images

EM Images (snakes on a grid) c5.0 barrel (10 nM), 0.7% uranyl formate Appear to be lining up end to end, probably because of the stain Images courtesy Shawn Douglas

To be continued protease Can a protein be protected from protease if attached inside the box? Lid attachment Lid removal protein protease protein

Acknowledgements Harvard TFs - Shawn Douglas, Nick Stroustrup, Chris Doucette Harvard advisers - Dr. William Shih, Dr. George Church, Dr. Pamela Silver, Dr. Alain Viel, Dr. Jagesh Shah, Dr. Radhika Nagpal iGEM ambassadors iGEM directors