Estimation of Carbohydrate by Anthrone Method The anthrone reaction is the basis of a rapid and convenient method for the determination of carbohydrates, either free or present in polysaccharides.

* Principle H2SO4 + Furfural Anthrone Blue Green Complex

Materials 1. Anthrone reagent (0.2% in conc. H2SO4 ). 2. Glucose (10mg/100ml). 3. Colorimeter or spectrophotometer.

COOL to Room Temperature Read the Optical Density at 620 nm Tube # Blank St. unk. 10 min. 60 30 15 45 5 10 20 25 35 40 50 55 Boiling Water Bath Glucose (ml) 0 0.5 0.5 D. W. (ml) 1 0.5 0.5 Anthrone (ml) 4 4 4 Mix Well COOL to Room Temperature Read the Optical Density at 620 nm

* Calculations CUn. = A Un. × C St. Absorbance of Unknown Absorbance of Standard = Concentration of Unknown Concentration of Standard CUn. = A Un. × C St. A St. Samar A. Damiati