Mandy G. Katz-Jaffe, Ph. D. , Mark G. Larman, Ph. D. , Courtney B

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Exposure of mouse oocytes to 1,2-propanediol during slow freezing alters the proteome Mandy G. Katz-Jaffe, Ph.D., Mark G. Larman, Ph.D., Courtney B. Sheehan, B.Sc., David K. Gardner, D.Phil. Fertility and Sterility Volume 89, Issue 5, Pages 1441-1447 (May 2008) DOI: 10.1016/j.fertnstert.2007.03.098 Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Profiling by CM10 of mouse MII oocytes in the molecular range of 2 to 20 kDa, enhanced around the region of 3.8 to 4 kDa. (A) Typical spectrum plotted by normalized peak intensity against mass to charge ratio. (B) Gel plots with peak height translated to band intensity. Group A, control; group B, temperature control; group C, equilibration and dehydration step; and group D, equilibration, dehydration, and seeding step. Peaks of interest are boxed, displaying down-regulation from control groups A and B to treatment groups C and D (P<.01). Fertility and Sterility 2008 89, 1441-1447DOI: (10.1016/j.fertnstert.2007.03.098) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Profiling by Q10 of mouse MII oocytes in the molecular range of 2 to 20 kDa, enhanced around the region of 15 to 19 kDa. (A) Typical spectrum plotted by normalized peak intensity against mass to charge ratio. (B) Gel plots with peak height translated to band intensity. Group A, control; group B, temperature control; group C, equilibration and dehydration step; and group D, equilibration, dehydration, and seeding step. Peaks of interest are boxed, displaying up-regulation from control groups A and B to treatment groups C and D (P<.01). Fertility and Sterility 2008 89, 1441-1447DOI: (10.1016/j.fertnstert.2007.03.098) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Box plots of differentially expressed proteins in MII oocytes after mimicking events of the slow-freezing protocol. (A) A 5.4-kDa protein up-regulated in MII oocytes after 20 minutes of PrOH exposure, either without or with seeding (∗∗P<.003). (B) A 5.1-kDa protein down-regulated in MII oocytes after 20 minutes of PrOH exposure, either without or with seeding (∗∗P<.005). Fertility and Sterility 2008 89, 1441-1447DOI: (10.1016/j.fertnstert.2007.03.098) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions