Resurrecting the Dodo: Positive control DNA for species identification

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Presentation transcript:

Resurrecting the Dodo: Positive control DNA for species identification L.M.I. Webster, R. McEwing  Forensic Science International: Genetics Supplement Series  Volume 4, Issue 1, Pages e140-e141 (January 2013) DOI: 10.1016/j.fsigss.2013.10.071 Copyright © 2013 Terms and Conditions

Fig. 1 Schematic layout of the Dodo2 artificial positive control sequence. Primer sequences from 4 different tests are concatenated onto either end of the Dodo cytochrome b fragment. A=animal cytochrome oxidase 1 [2], B=dog-specific control region [4], C=animal cytochrome b [3], and D=caviar cytochrome b [5]. Forensic Science International: Genetics Supplement Series 2013 4, e140-e141DOI: (10.1016/j.fsigss.2013.10.071) Copyright © 2013 Terms and Conditions

Fig. 2 Schematic of PCR product lengths as if separated by electrophoresis using positive control DNA (grey bar) and the Dodo2 fragment amplified by each primer set (coloured bars). A=animal cytochrome oxidase 1 [2], B=dog-specific control region [4], C=animal cytochrome b [3], and D=caviar cytochrome b [5]. Forensic Science International: Genetics Supplement Series 2013 4, e140-e141DOI: (10.1016/j.fsigss.2013.10.071) Copyright © 2013 Terms and Conditions