Use of short-term culture for identification of Mycobacterium avium subsp. paratuberculosis in tissue from Crohn's disease patients  D. Schwartz, I. Shafran,

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Use of short-term culture for identification of Mycobacterium avium subsp. paratuberculosis in tissue from Crohn's disease patients  D. Schwartz, I. Shafran, C. Romero, C. Piromalli, J. Biggerstaff, N. Naser, W. Chamberlin, S.A. Naser  Clinical Microbiology and Infection  Volume 6, Issue 6, Pages 303-307 (June 2000) DOI: 10.1046/j.1469-0691.2000.00093.x Copyright © 2000 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 1 Microscopic analysis of short-term culture of a resected tissue specimen from a CD patient. Acid-fast stain was performed on a culture sample obtained following short-term incubation of processed CD tissue. (A) Corresponds to the prespheroplastic form of MAP with partial cell wall materials. (B) Corresponds to the intact bacillus form of MAP. Clinical Microbiology and Infection 2000 6, 303-307DOI: (10.1046/j.1469-0691.2000.00093.x) Copyright © 2000 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 2 IS900-PCR detection of MAP from surgically resected tissue cultured in MGIT media. PCR products were analyzed on 2.0% agarose gel. Lane 1 represents DNA from MAP strain 43015. Lane 2 has no DNA. Lanes 3–9 represent resected tissue from CD patients. Lanes 10–12 represent tissue from controls. M is the molecular weight marker in base pairs (bp). Clinical Microbiology and Infection 2000 6, 303-307DOI: (10.1046/j.1469-0691.2000.00093.x) Copyright © 2000 European Society of Clinical Infectious Diseases Terms and Conditions

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