IraL affects the levels and stability of σS during log phase growth.

Slides:



Advertisements
Similar presentations
Fig. 3. In vitro and in vivo activity of Cgs CR I mutants
Advertisements

SUPPLEMENTAL FIGURE S1. Growth in BHI after 12 days at 4°C of L
Genetic alterations in the PI3K–PTEN–AKT pathway detected during disease progression and their functional impacts. Genetic alterations in the PI3K–PTEN–AKT.
Volume 22, Issue 3, Pages (May 2006)
David X Liu, Lloyd A Greene  Neuron 
Volume 36, Issue 2, Pages (October 2009)
Volume 86, Issue 6, Pages (September 1996)
Volume 6, Issue 4, Pages (October 2000)
Volume 123, Issue 3, Pages (September 2002)
Cellular DDX21 RNA Helicase Inhibits Influenza A Virus Replication but Is Counteracted by the Viral NS1 Protein  Guifang Chen, Chien-Hung Liu, Ligang.
Takashi Kubota, Kohei Nishimura, Masato T. Kanemaki, Anne D. Donaldson 
Volume 12, Issue 2, Pages (August 2003)
The ΔrlmA mutant strain has impaired CWI pathway activation.
HUWE1 regulates ubiquitination of Shoc2 and RAF-1.
Allele-specific inhibitors inactivate mutant KRAS G12C by a trapping mechanism by Piro Lito, Martha Solomon, Lian-Sheng Li, Rasmus Hansen, and Neal Rosen.
A Truncated Arabidopsis NUCLEOSOME ASSEMBLY PROTEIN 1, AtNAP1;3T, Alters Plant Growth Responses to Abscisic Acid and Salt in the Atnap1;3-2 Mutant  Liu.
Hery Ratsima, Diego Serrano, Mirela Pascariu, Damien D’Amours 
MiR-34a contributes to megakaryocytic differentiation of K562 cells independently of p53 by Francisco Navarro, David Gutman, Eti Meire, Mario Cáceres,
The complex containing actin-related proteins Arp2 and Arp3 is required for the motility and integrity of yeast actin patches  Dirk Winter, Alexandre.
MUC1 Oncoprotein Stabilizes and Activates Estrogen Receptor α
Cell-Density-Dependent Regulation of Expression and Glycosylation of Dopachrome Tautomerase/Tyrosinase-Related Protein-2  Thomas J. Hornyak, Daniel J.
Last class Salting out Dialysis Paper 1 discussion.
RRNA Modifications in an Intersubunit Bridge of the Ribosome Strongly Affect Both Ribosome Biogenesis and Activity  Xue-hai Liang, Qing Liu, Maurille.
Patterns of replication within tissue cysts.
Volume 6, Issue 4, Pages (October 2000)
Cdc7-Dbf4 Phosphorylates MCM Proteins via a Docking Site-Mediated Mechanism to Promote S Phase Progression  Yi-Jun Sheu, Bruce Stillman  Molecular Cell 
Volume 77, Issue 4, Pages (February 2010)
MUC1 Oncoprotein Stabilizes and Activates Estrogen Receptor α
ERMES impacts on the exposure of pathogen-associated molecular patterns during hyphal growth. ERMES impacts on the exposure of pathogen-associated molecular.
Volume 17, Issue 1, Pages (January 2005)
Growth of L. monocytogenes strains AL4E, AT3E, and 10403S, derivative cured strain AT3Epc, and conjugation strains 10403SpclpL and 10403SpPL2 displayed.
Positive or Negative Roles of Different Cyclin-Dependent Kinase Pho85-Cyclin Complexes Orchestrate Induction of Autophagy in Saccharomyces cerevisiae 
Volume 6, Issue 1, Pages (July 2009)
Effects of ERK–GFP expression levels and cell density on ERK phosphorylation and oscillations. Effects of ERK–GFP expression levels and cell density on.
Activation of PPARγ leads to inhibition of anchorage-independent growth of human colorectal cancer cells  Jeffrey A. Brockman, Rajnish A. Gupta, Raymond.
IraL interacts with RssB.
Characterization of Keratinocyte Differentiation Induced by Ascorbic Acid: Protein Kinase C Involvement and Vitamin C Homeostasis1  Isabella Savini, Antonello.
Distinct Pathways for snoRNA and mRNA Termination
Rsk1 mediates a MEK–MAP kinase cell survival signal
Volume 3, Issue 5, Pages (September 2010)
Light-Dependent Interactions between the Drosophila Circadian Clock Factors Cryptochrome, Jetlag, and Timeless  Nicolai Peschel, Ko Fan Chen, Gisela Szabo,
Regulation of the Drosophila Protein Timeless Suggests a Mechanism for Resetting the Circadian Clock by Light  Melissa Hunter-Ensor, Andrea Ousley, Amita.
iraL shares sequence identity with iraM from E
Volume 18, Issue 20, Pages (October 2008)
Jodi L. Vogel, Dawn A. Parsell, Susan Lindquist  Current Biology 
Identification of the uvh6-4 mutation.
Dap1/PGRMC1 Binds and Regulates Cytochrome P450 Enzymes
Immunoblot analysis of GpGCS1 expression in strains K41 (mating-type plus) and K34 (mating-type minus). Immunoblot analysis of GpGCS1 expression in strains.
Binning by cross-competition ELISAs of cluster II mAbs.
Stability of SsbA (A) and GFP (B) proteins in UA159 and ΔclpX strains.
E. coli CFT073 and E. coli MG1655 persistence.
ESCRT complexes are required for efficient consumption of LDs
E4-ORF1 binds endogenous PATJ and redistributes it into detergent-insoluble complexes in MDCK cells. E4-ORF1 binds endogenous PATJ and redistributes it.
WW domain does not show dominant-negative effect on TBSV replication in a yeast cell extract. WW domain does not show dominant-negative effect on TBSV.
Target versatility. Target versatility. (A) Δctl0063, Δctl0064, Δctl0065, ΔtrpA, and wild-type C. trachomatis were analyzed by PCR with primers immediately.
DncV downregulates motility in ECOR31 downstream of transcription of the E. coli master regulator FlhD4C2 of the class 1 flagellum regulon. dncV downregulates.
(A) Comparison of mcr-9 to all previously described mcr homologues, based on amino acid sequence. (A) Comparison of mcr-9 to all previously described mcr.
SIN4 function was required for the entry into a normal stationary phase. SIN4 function was required for the entry into a normal stationary phase. (A) The.
Fig. 4 Enzyme activity of PE-ProK/Pt tuned by changing temperature or NIR light. Enzyme activity of PE-ProK/Pt tuned by changing temperature or NIR light.
Dual Toxic-Peptide-Coding Staphylococcus aureus RNA under Antisense Regulation Targets Host Cells and Bacterial Rivals Unequally  Marie-Laure Pinel-Marie,
The Engagement of Sec61p in the ER Dislocation Process
Fig. 5 Dcr-2 directly binds to Toll 3′UTR.
Chih-Yung S. Lee, Tzu-Lan Yeh, Bridget T. Hughes, Peter J. Espenshade 
Volume 11, Issue 2, Pages (February 2018)
Impact of the SOS activities on E. coli mutation rates.
Dietary choline is required for TMAO accumulation.
Percentage of papers containing inappropriate image duplications by year of publication. Percentage of papers containing inappropriate image duplications.
Expression of MtrE by wild-type and mtr120 mutant strains.
Clade 2 ERFs Differentially Bind and Activate a PMT2 Promoter
Effect of 1% (wt/vol) aqueous suspensions of KC on the viability of various ESKAPE strains. Effect of 1% (wt/vol) aqueous suspensions of KC on the viability.
Presentation transcript:

IraL affects the levels and stability of σS during log phase growth. IraL affects the levels and stability of σS during log phase growth. (A) To assess the effects of IraL on σS levels, CFT073, CFT073 ΔiraL, S. sonnei, S. sonnei ΔiraL, K-12/pACYC184 (vector only), and K-12/pIraL (pACYC with iraL under the control of its native promoter) were grown to the log phase (LOG) or the stationary phase (STAT) and immunoblot assays for σS were carried out with 10 µg of total soluble protein from these samples. For accompanying Coomassie blue-stained gels that illustrate consistent loading of the protein samples, see Fig. S4 in the supplemental material. Marker (M) lanes contain the Precision Plus Protein Dual Color Standard that cross-reacts with the detection reagents used. (B) To assess the effects of IraL on σS stability, CFT073 and isogenic mutants were grown to the mid-log phase as described for panel A, protein synthesis was stopped with Cm, and samples of total protein were precipitated in 10% TCA at 3-min intervals after Cm treatment. Immunoblot assays were carried out with total protein normalized to the OD600, and σS levels were measured by densitometry. Data points represent the mean percentages of σS remaining relative to those at t = 0, and error bars represent ± the standard error of the mean of three replicates. Andrew J. Hryckowian et al. mBio 2014; doi:10.1128/mBio.01043-14