Sharon J Paynter, Ph. D. , Louise O’Neil, Ph. D

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Presentation transcript:

Membrane permeability of human oocytes in the presence of the cryoprotectant propane-1,2-diol  Sharon J Paynter, Ph.D., Louise O’Neil, Ph.D., Barry J Fuller, Robert W Shaw, M.D.  Fertility and Sterility  Volume 75, Issue 3, Pages 532-538 (March 2001) DOI: 10.1016/S0015-0282(00)01757-X

Figure 1 Mean (±SD) osmotic response of human oocytes after exposure to 1.5 M propane-1,2-diol. Oocytes were exposed at 10°C (♦), 24°C (□), and 30°C (■). The solid lines represent the mean predicted osmotic response for each case. Paynter. Oocyte membrane permeability and cryoprotectant. Fertil Steril 2001. Fertility and Sterility 2001 75, 532-538DOI: (10.1016/S0015-0282(00)01757-X)

Figure 2 Arrenhius plot of mean (±SD) Lp (□) and Ppropane-1,2-diol (♢) data of human oocytes in the presence of 1.5 M propane-1,2-diol. Paynter. Oocyte membrane permeability and cryoprotectant. Fertil Steril 2001. Fertility and Sterility 2001 75, 532-538DOI: (10.1016/S0015-0282(00)01757-X)

Figure 3 Predicted osmotic response of human oocyte exposed to 1.5 M propane-1,2-diol for 10 minutes at 24°C followed by exposure to 1.5 M propane-1,2-diol plus either 0.1 M or 0.2 M (heavy solid line) sucrose at 24°C. Paynter. Oocyte membrane permeability and cryoprotectant. Fertil Steril 2001. Fertility and Sterility 2001 75, 532-538DOI: (10.1016/S0015-0282(00)01757-X)

Figure 4 Measured osmotic response of human oocyte exposed to 1.5 M propane-1,2-diol plus 0.1 M sucrose at 24°C (□), having been equilibrated previously with 1.5 M propane-1,2-diol. The solid line represents the predicted osmotic response. Paynter. Oocyte membrane permeability and cryoprotectant. Fertil Steril 2001. Fertility and Sterility 2001 75, 532-538DOI: (10.1016/S0015-0282(00)01757-X)