Live‐cell imaging uncovers a slowly cycling drug‐resistant state involved in adaptation to RAF inhibition Live‐cell imaging uncovers a slowly cycling drug‐resistant.

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Live‐cell imaging uncovers a slowly cycling drug‐resistant state involved in adaptation to RAF inhibition Live‐cell imaging uncovers a slowly cycling drug‐resistant state involved in adaptation to RAF inhibition Time‐lapse imaging of COLO858 and MMACSF cells stably expressing H2B‐Venus and mCherry‐geminin exposed to 1 μM vemurafenib or DMSO for 4–8 days. A, BRepresentative images and cell cycle phases (A) and representative maps of cell lineage (B) are depicted for COLO858 under DMSO and vemurafenib conditions.CSingle‐cell analysis of division and death events. Horizontal axes represent single‐cell tracks with time. Division events are displayed as black or pink (in the case of slowly cycling cells) dots. Transition from yellow to gray indicates cell death.DPercentage of surviving and dead cells among cells tracked for 84 h.EPercentage of division events among cells tracked during indicated time intervals.FLength of different cell cycle phases (G0/G1 and S/G2) in cells tracked for 84 h. No data are reported for MMACSF‐vemurafenib because all cells stopped dividing ˜24 h after treatment and no single cell divided more than once.GDivision times for COLO858 cells tracked between days 4 and 8 post‐treatment with 1 μM vemurafenib. Minimum doubling times were estimated for 100 individual cells by identifying the longest time interval before or after which a cell divides.Data information: Data in (D–F) are presented as mean ± SD using 3–4 groups of cells imaged from multiple wells (see Materials and Methods). Mohammad Fallahi‐Sichani et al. Mol Syst Biol 2017;13:905 © as stated in the article, figure or figure legend