Effects of XIST on glioma cell proliferation and chemoresistance to TMZ Effects of XIST on glioma cell proliferation and chemoresistance to TMZ (A) siRNA-NC/siRNA-XIST.

Slides:

Advertisements

Similar presentations

Selective inhibition of BCL10-induced NF-κB activation by Tat–Peptide VIII HEK-293 cells were transiently co-transfected with an expression vector encoding.

Advertisements

Nicotinamide Phosphoribosyltransferase: A Potent Therapeutic Target in Non-small Cell Lung Cancer with Epidermal Growth Factor Receptor-Gene Mutation

ABAE positive cells 6h after treatment

The Long Non-Coding RNA XIST Interacted with MiR-124 to Modulate Bladder Cancer Growth, Invasion and Migration by Targeting Androgen Receptor (AR) Cell.

IDH1 mutant glioma cells are sensitive to TMZ

Effects of BRG1 depletion in the proliferation of lung cancer cells with amplification at the MYC family of genes and in MAX-deficient cells, after reconstitution.

CDK2 is highly expressed in colon cancer cells and curcumin selectively suppresses HCT116 cell proliferation. CDK2 is highly expressed in colon cancer.

PKCK2 determines TRAIL sensitivity.

NF1 silencing confers resistance of PC9 lung adenocarcinoma cells to erlotinib (erl). NF1 silencing confers resistance of PC9 lung adenocarcinoma cells.

FL cells are dependent on BCL6 in a NOTCH2-dependent manner.

A FOXO3a-BIM cascade mediates sensitivity to PARP and MEK inhibition

A B 20% O2 5% O2 C 5% O2 + NAC DAPI gH2AX pLKO.1 GFP-SOD1 S 0.1Gy

SP1 was a downstream target of miR-150-3p

Pioglitazone inhibits aromatase expression in human preadipocytes.

Decreased expression of FOXA2 promotes eutopic endometrial cell proliferation and migration in patients with endometriosis Anping Lin, Juan Yin, Chao.

BIRC6 confers resistance against cisplatin and oxaliplatin.

Knockdown of DAO inhibits DNA damage–induced senescence.

Supplementary Fig. S1 BCL2 5’flanking ctrl siRNA luc

Changes in transcript abundance of the TR-ACS gene family in white clover roots over 24 h in response to Pi supply Changes in transcript abundance of the.

Nicotinamide Phosphoribosyltransferase: A Potent Therapeutic Target in Non-small Cell Lung Cancer with Epidermal Growth Factor Receptor-Gene Mutation

XIST regulated miR-29c by directly targetting in TMZ-resistant glioma cells XIST regulated miR-29c by directly targetting in TMZ-resistant glioma cells.

An siRNA screen identifies BER pathway members as sensitizers to temozolomide (TMZ) in pediatric GBM. A and B, SJG2 and KNS42 cells were transfected with.

Overexpression of miR-340 inhibited osteoclast differentiation

A and B, CO-1686–resistant NCI-H1975 cell clones, COR1-1 and COR10-1 display a reduced dependence on EGFR signaling for survival. A and B, CO-1686–resistant.

Fig. 2. Cellular response to FolamiRs in vitro.

Volume 18, Issue 3, Pages (March 2010)

TRAF4 is required for EGFR activation in response to EGF stimulation.

The effects of KY19382 on chondrocyte proliferation and differentiation. The effects of KY19382 on chondrocyte proliferation and differentiation. (A) ATDC5.

Knocking down Wnt3 increases the cells' response to trastuzumab and reduces cells' invasiveness. Knocking down Wnt3 increases the cells' response to trastuzumab.

Effect of insulin on hepcidin expression in HepG2 cells.

JAK3A572V mutation causes constitutive JAK3 activity and IL-2–independent proliferation of NKTCL cells. JAK3A572V mutation causes constitutive JAK3 activity.

CREB1 promotes the induction of endogenous ERα target genes.

Effect of the siRNA-mediated knockdown of endogenous MARCH8 on the expression levels of MARCH8 substrates, TfR and CD98, in HepG2 cells. Effect of the.

MiR-431-5p directly targets RAF1 and is negatively correlated to RAF1 expression (A) The predicted binding sites of miR-431-5p and RAF1 3′-UTR, and mutant.

FBXL19-AS1 acts as a sponge of miR-431-5p in lung cancer (A) The predicted binding sites of FBXL19-AS1 and miR-431-5p, and the mutant sites in mutant-type.

Downregulation of caveolin-1 by si-RNA in NIH3T3 cells increased survivin expression. Downregulation of caveolin-1 by si-RNA in NIH3T3 cells increased.

TSC1 knockdown enhances hypoxia-mediated lymphocyte death via mTORC1 activation TSC1 knockdown enhances hypoxia-mediated lymphocyte death via mTORC1 activation.

Engagement of PDGF-αR and PDGF-βR is critical for PDGF-BB-mediated increased proliferation of NPCs. RT-PCR (A) and WB (B) of PDGF-αR and PDGF-βR in NPCs;

Hypoxia decreases lipogenesis via mTORC1 signalling in lymphocytes

Aggregation and toxicity of α-synuclein in HEK293T cells.

TFAP2A interacts with HIF-1α and regulates VEGF expression and cell proliferation. TFAP2A interacts with HIF-1α and regulates VEGF expression and cell.

Induction of CDCP1 is regulated by Ras in NSCLC cells.

Silencing hOGG1 triggers caspase-3 and caspase-7 activation in response to H2O2 in GM00637 cells. Silencing hOGG1 triggers caspase-3 and caspase-7 activation.

The role of p53 in H2O2-mediated cell death of hOGG1-deficient H1299 lung carcinoma p53 null cells. The role of p53 in H2O2-mediated cell death of hOGG1-deficient.

A, chemical structures of PRIMA-1 and PRIMA-1Met.

PELP1 regulates the expression and activities of MMPs in ER-negative cells. PELP1 regulates the expression and activities of MMPs in ER-negative cells.

Β-Cryptoxanthin at a concentration of 10 μmol/L decreases proliferation in HCT116 cells after 6 and 8 days of treatment. β-Cryptoxanthin at a concentration.

The effects of IAA and glycyrrhizin (Gc) on proliferation and cell-cycle distribution. The effects of IAA and glycyrrhizin (Gc) on proliferation and cell-cycle.

Effects of visfatin on the cell proliferation and phosphorylation of ERK, Akt, and GSK-3β proteins in HCC cells. Effects of visfatin on the cell proliferation.

Bcl-2 and caspase inhibition fails to inhibit BAMLET-induced cytotoxicity in MCF-7 cells. Bcl-2 and caspase inhibition fails to inhibit BAMLET-induced.

The effect of IAA and glycyrrhizin (Gc) on growth of human melanoma cells. The effect of IAA and glycyrrhizin (Gc) on growth of human melanoma cells. A,

HNSCC and NSCLC cell lines display differential sensitivities to cixutumumab in the 3D mimic condition. HNSCC and NSCLC cell lines display differential.

TFAP2A knockdown inhibits cell growth by targeting HIF-1α signaling.

CDCP1 links Ras and SFK signaling and regulates anoikis resistance, cell migration, and invasion in NSCLC cells. CDCP1 links Ras and SFK signaling and.

Per1 inhibits growth and induces apoptosis in prostate cancer cell lines. Per1 inhibits growth and induces apoptosis in prostate cancer cell lines. LNCaP,

The effect of different concentrations of WMC-79 and time of exposure on the growth of HCT-116 cells. The effect of different concentrations of WMC-79.

Platinum-based chemotherapy increased TXNIP expression, and overexpression of TXNIP enhanced the effectiveness of this therapy. Platinum-based chemotherapy.

FBXL19-AS1 knockdown inhibits proliferation, migration and invasion, and reduces the expression levels of angiogenesis related proteins in lung cancer.

Identification of compounds that enhance TMZ cytotoxicity in melanoma cells by screening the Spectrum Collection library. Identification of compounds that.

AS1411 alters subcellular distribution of PRMT5 in a time-dependent, dose-dependent, and nucleolin-dependent manner. AS1411 alters subcellular distribution.

Hcy decreases VEGFR1/2 and Angs (Ang1, Ang2) gene transcription

ABT-888 sensitizes multiple ovarian cancer cell lines but not normal cells to FdUrd. ABT-888 sensitizes multiple ovarian cancer cell lines but not normal.

Autophagy mediates postslippage cell fate.

PLK1 is a crucial downstream effector of PDK1 for MYC activation and cell survival. PLK1 is a crucial downstream effector of PDK1 for MYC activation and.

Curcumin decreases viability and proliferation of Bcr-Abl-expressing cells. Curcumin decreases viability and proliferation of Bcr-Abl-expressing cells.

The cytotoxicity of Bacillus subtilis or surfactin in IPEC-J2 cells.

Wnt5A and ROR2 contribute to intrinsic resistance to BRAF inhibitors.

DQs have superior anticancer efficacy among dimeric antimalarials.

CREB1 binds at the proximal region of the TGFB2 promoter and induces its transcriptional activation. CREB1 binds at the proximal region of the TGFB2 promoter.

MYC is required for the hypoxic induction of SHMT2.

Presentation transcript:

Effects of XIST on glioma cell proliferation and chemoresistance to TMZ Effects of XIST on glioma cell proliferation and chemoresistance to TMZ (A) siRNA-NC/siRNA-XIST was transfected into LN229 and U251 cells. The expression of XIST was verified using real-time PCR assays. (B,C) After transfection with the indicated vectors, the cell viability of LN229 and U251 cells was determined using MTT assays. (D) After transfection with the indicated vectors, the cell proliferation of LN229 and U251 cells was determined using BrdU assays. (E,F) LN229 and U251 cells were transfected with the indicated vectors. Twenty four hours post-transfection, cells were treated with a series dose of TMZ (0, 30, 60, 120, 240 μM) for 24 h. The cell viability was determined using MTT assays, and the data were presented as a percentage normalized to the viability of cells with 0 μM TMZ treatment. The data are presented as mean ± S.D. of three independent experiments; *P<0.05, **P<0.01. (G,H) LN229, TMZ-resistant LN229 (LN229/TMZ), U251, TMZ-resistant U251 (U251/TMZ) cells were treated with a series dose of TMZ (7.5, 15, 30, 60, 120, 240, and 480 μM) for 24 h, and the cell viability of the indicated cells was determined using MTT assays. Data were displayed as a percentage normalized to the viability of cells with no TMZ treatment. The abscissa was the logarithm of TMZ concentration (log-conc.). LC50 represented the concentration of TMZ when cell viability was reduced to 50%. (I,J) LN229/TMZ and U251/TMZ cells were transfected with siRNA-NC/siRNA-XIST. Twenty four hours after transfection, cells were treated with a series dose of TMZ (7.5, 15, 30, 60, 120, 240, and 480 μM) for another 24 h; the cell viability was then determined using MTT assays. Peng Du et al. Biosci. Rep. 2017;37:BSR20170696 ©2017 by Portland Press Ltd