Volume 7, Issue 6, Pages (June 2000)

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Volume 7, Issue 6, Pages 395-403 (June 2000) Biosynthesis of the polyene antifungal antibiotic nystatin in Streptomyces noursei ATCC 11455: analysis of the gene cluster and deduction of the biosynthetic pathway Trygve Brautaset, Olga N Sekurova, Håvard Sletta, Trond E Ellingsen, Arne R Strøm, Svein Valla, Sergey B Zotchev Chemistry & Biology Volume 7, Issue 6, Pages 395-403 (June 2000) DOI: 10.1016/S1074-5521(00)00120-4

Figure 1 Structure of the nystatin A1 molecule according to Lancelin and Beau [36]. Chemistry & Biology 2000 7, 395-403DOI: (10.1016/S1074-5521(00)00120-4)

Figure 2 Gene organization within the S. noursei ATCC 11455 nystatin biosynthetic gene cluster. The inserts from the overlapping recombinant phages encompassing the cloned region are shown above the physical/genetic map. The nys genes are designated with capital letters in italics, other ORFs are numbered. Stars above certain ORFs indicate that these genes were successfully disrupted (see the text and Table 1 for details). Chemistry & Biology 2000 7, 395-403DOI: (10.1016/S1074-5521(00)00120-4)

Figure 3 Functional organization of the nystatin PKS including the NysA, NysB, NysC, NysI, NysJ and NysK proteins. KS, ketosynthase; KSS, ketosynthase with the Cys→Ser substitution in the active site; AT, acetate-specific acyltransferase; mAT, propionate-specific acetyltransferase; DH, dehydratase; DHi, inactive dehydratase; ER, enoyl reductase; KR, ketoreductase; KRi, inactive ketoreductase; ACP, acyl carrier protein. Chemistry & Biology 2000 7, 395-403DOI: (10.1016/S1074-5521(00)00120-4)

Figure 4 Proposed model for nystatin biosynthesis in S. noursei. Chemistry & Biology 2000 7, 395-403DOI: (10.1016/S1074-5521(00)00120-4)