Fractional clearance of high molecular weight proteins in conscious rats using a continuous infusion method  Melissa J. Burne, Tanya M. Osicka, Wayne D. Comper  Kidney International  Volume 55, Issue 1, Pages 261-270 (January 1999) DOI: 10.1046/j.1523-1755.1999.00234.x Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 1 Calibration of Sephacryl S-200 column with narrow molecular weight dextran samples. Kidney International 1999 55, 261-270DOI: (10.1046/j.1523-1755.1999.00234.x) Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 2 Plasma concentrations of (A) glucose oxidase, (B) IgG, (C) lactate dehydrogenase, (D) lactoperoxidase, (E) transferrin, and (F) albumin with continuous infusion using ALZET osmotic pumps over a period of 7 days. Tracer was released at 1 μl/hr (N = 5 for each protein). Kidney International 1999 55, 261-270DOI: (10.1046/j.1523-1755.1999.00234.x) Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 3 Fractional clearance of (A) glucose oxidase, (B) IgG, (C) lactate dehydrogenase, (D) lactoperoxidase, (E) transferrin, and (F) albumin over a period of 7 days (N= 5 for each protein). Kidney International 1999 55, 261-270DOI: (10.1046/j.1523-1755.1999.00234.x) Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 4 Representative profiles of the size exclusion chromatography of [3H]proteins in urine (○) and plasma (•) samples. (A) glucose oxidase (urine loaded = 28,000 dpm, plasma loaded = 18,000 dpm), (B) IgG (urine loaded = 15,000 dpm, plasma loaded = 8,000 dpm), (C) lactate dehydrogenase (urine loaded = 25,000 dpm, plasma loaded = 10,000 dpm), (D) lactoperoxidase (urine loaded = 35,000 dpm, plasma loaded = 15,000 dpm), (E) transferrin (urine loaded = 40,000 dpm, plasma loaded = 16,000 dpm) and (F) albumin (loaded on a G-100; urine loaded = 30,000 dpm, plasma loaded = 45,000 dpm). Plasma samples were obtained from the osmotic pump at day 7 and urine samples were obtained two hours following the bolus intravenous injection of the [3H]proteins. Kidney International 1999 55, 261-270DOI: (10.1046/j.1523-1755.1999.00234.x) Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 5 The fractional clearance of [3H]proteins on the basis of hydrodynamic radius (N= 5 for each protein). Symbols are: (•) albumin, (▪) transferrin, (▴) lactate dehydrogenase, (▾) IgG, (♦) lactoperoxidase and (filled hexagon shape) glucose oxidase. Kidney International 1999 55, 261-270DOI: (10.1046/j.1523-1755.1999.00234.x) Copyright © 1999 International Society of Nephrology Terms and Conditions