Multiplex PCR Detection of GSTM1, GSTT1, and GSTP1 Gene Variants

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Multiplex PCR Detection of GSTM1, GSTT1, and GSTP1 Gene Variants Anders Buchard, Juan J. Sanchez, Kim Dalhoff, Niels Morling The Journal of Molecular Diagnostics Volume 9, Issue 5, Pages 612-617 (November 2007) DOI: 10.2353/jmoldx.2007.070030 Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Amplification strategy for the detection of the GSTT1/GSTM1 deletion. If the GSTT1/GSTM1 is present, the DNA fragment between the forward and reverse primers is too long for amplification. If the GSTT1/GSTM1 is deleted, amplification will occur. The Journal of Molecular Diagnostics 2007 9, 612-617DOI: (10.2353/jmoldx.2007.070030) Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Multiplex PCR products of 10 samples electrophoresed on a 1.5% agarose gel. For each individual, it was possible to determine the GSTM1 and GSTT1 genotypes by the presence or absence of the relevant amplification products. The DNA of the individual in lane 1, for example, was heterozygous for GSTM1 and homozygous for the deletion in GSTT1. The DNA of the individual in lane 2 had two copies of both GSTM1 and GSTT1 because amplification products for both genes were present, whereas no amplification product was present corresponding to the deletions. The 1-kb Plus DNA Ladder (Invitrogen) was used as reference. The Journal of Molecular Diagnostics 2007 9, 612-617DOI: (10.2353/jmoldx.2007.070030) Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 3 Electropherogrammes of GSTT1, GSTM1, and GSTP1 typing in two individuals. The first individual was heterozygous for GSTM1, GSTT1, and GSTP1. The second individual was homozygous for the deletion in GSTM1 and GSTT1 and heterozygous for GSTP1. The Journal of Molecular Diagnostics 2007 9, 612-617DOI: (10.2353/jmoldx.2007.070030) Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions