Delivery of therapeutics to patients.

Slides:

Advertisements

Similar presentations

Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA Telephone   Fax   

Advertisements

Date of download: 10/9/2017 Copyright © ASME. All rights reserved.

Effects of inhibiting inflammatory cell recruitment on fracture healing Treatment with CCR2 antagonist, INCB3344, led to impaired fracture healing compared.

Date of download: 10/16/2017 Copyright © ASME. All rights reserved.

Production and clinical development of nanoparticles for gene delivery

miRNAs regulated by c‐Myc in murine ES cells.

Volume 158, Issue 6, (September 2014)

MiR‐10b* down‐regulates the expression of BUB1, PLK1 and CCNA2 proteins in breast cancer cell lines.A.SPIN‐ordered expression matrix of miR‐10b* and its.

Figure 5 Two approaches to therapeutic genome editing

GLUT3 is a direct target of TCF4/β‐catenin

The gene encoding TP53INP1 is expressed in pancreatic endocrine cells A, B(A, B) Immunocytofluorescent staining of TP53INP1 (red) and insulin (green) in.

Treatment with p38γ/p38δ inhibitor shows antifungal effects in vivo

The mRNA stem cell signature.

Generation and characterization of Vps15‐null mice and cells. A

Roles of systemic versus tumour SK1 in the regulation of local tumour growth and lung colonization/metastasis.A.Effects of siRNA‐mediated SK1 knockdown.

CAR T cell dose and percentages of CAR‐positive cells within CAR T cell products CAR T cell dose and percentages of CAR‐positive cells within CAR T cell.

RNA interference: the new somatic cell genetics?

Both haematopoietic and non‐haematopoietic compartments are involved in myocarditis development.A.Kaplan‐Meier survival curves showing survival of bone.

Gene therapy in haematology and oncology

Preceding knockdown of FoxO3 reduces inhibitory effect of UCN‐01 on human lung fibroblast proliferation and myofibroblast transdifferentiation in response.

In vitro and in vivo validation of Clcn3 as a miR‐1a‐3p target gene and its functional contribution to tumor‐induced mechanical hypersensitivity in the.

Phenogenetic networks of genes linked to Alzheimer's and Parkinson's disease reveal concordant phenotypes Phenogenetic networks of genes linked to Alzheimer's.

PolyQ‐huntingtin promotes membrane ErbB2/HER2 accumulation and sustained signalling.A.Immunostaining of sections from MMTV‐PyVT/HdhQ7/Q7 (HdhQ7/Q7) and.

Clinical testing of calcineurin inhibitors cyclosporine A and tacrolimus in autoimmune disorders Clinical testing of calcineurin inhibitors cyclosporine.

miR‐10b locus is hypermethylated in breast cancer. A

Stat3 promotes lung fibrosis and collagen synthesis independent of Smad3.A.Masson's trichrome stained sections of lung from gp130wt (wt), Smad3−/− (Smad3−/−),

SPP1 and TNC are induced by active JNK and promote lung metastasis

Selective biodistribution and receptor‐dependent uptake of T22‐GFP‐H6‐FdU in CXCR4+ cells in vivo Selective biodistribution and receptor‐dependent uptake.

RASGRP1 is required for T‐cell expansion in response to CD3‐TCR activation RASGRP1 is required for T‐cell expansion in response to CD3‐TCR activation Rescue.

Inhibition of JNK signaling in breast cancer cells and lung colonization in a xenograft mouse model Inhibition of JNK signaling in breast cancer cells.

Expression of PD‐1 on PBMC and malignant exudate T cells

Identification of miR‐499 targets

NRP2 induces expression of GLI1. A,B

Combination of OTX‐015 and PD does not induce overt toxicity

USP9X and XIAP promote lymphoma growth and resistance to spindle poisons in vivo USP9X and XIAP promote lymphoma growth and resistance to spindle poisons.

Schematic diagram of the proposed model depicting the functional relationship between EGFR and FASN in TKI resistance within EGFR mutant NSCLC cells Schematic.

PolyQ‐huntingtin accelerates EMT. A

Activation of inflammasomes by EBV‐associated factors and microenvironmental stressors.A.Expression of inflammasome components in NPC cell lines. Activation.

Association of AIM2, RIG‐I and NLRP3 inflammasomes with better survival in NPC patients.A.Overexpression of ASC, caspase‐1 and IL‐1β proteins as well as.

SPP1 and TNC are induced by chemotherapy via JNK signaling

The history of EMT in cancer.

The ERK pathway mediates the effect of EGF on LIMT, which normally inhibits mammary cell migration and invasion The ERK pathway mediates the effect of.

Improvement of shHBV7 specificity and efficiency by co‐expression of a TuD against the sense strand Improvement of shHBV7 specificity and efficiency by.

Knocking down Disease with siRNAs

Hccs downregulation induces apoptosome‐independent caspase‐9 activation in mitochondria.A–C.TUNEL assays on st30 embryos co‐injected with hccs‐MO and Apaf1‐MO.

Model of the BET and MEK inhibitor combination therapy to treat melanoma with NRAS mutation and immune therapy resistance Model of the BET and MEK inhibitor.

Inhibition of NAMPT using the potent inhibitor FK866 sensitizes additional tumour cells to olaparib.A‐E.Sensitivity of the cell lines MDAMB468 (A), SUM149.

Association of total disease burden and disease progression with age‐adjusted blood heteroplasmy Association of total disease burden and disease progression.

Association of total disease burden with age, m

CRISPR/Cas9: A Potential Life-Saving Tool. What’s next?

In vivo efficacy of Olaparib in PTEN deficient xenografts.

Gene targeting of the therapeutic EGFP/PGK‐FANCA donor in the AAVS1 locus of HD CD34+ cells Gene targeting of the therapeutic EGFP/PGK‐FANCA donor in the.

Induction of LIMD1 in hypoxia inhibits HIF‐1‐mediated gene expression

GPRC5A is hypoxia/HIF‐induced in vivo

AAV‐mediated photoreceptor ZFP gene transfer reduces the severity of adRP retinal dystrophy. AAV‐mediated photoreceptor ZFP gene transfer reduces the severity.

C266S laforin's effect on LD‐associated general defect in autophagy

Combined targeting of PCa AR and CCL2/CCR2 axis suppresses tumour growth and reduces metastasis in a xenograft mouse PCa model Proliferation assay of TRAMP‐C1.

Assessment of immortalisation, tumorigenicity and disseminative potential of the corrected stem cells Upper panel: expression of proteins associated with.

VCN and GFP distribution in LV.GFP‐injected NHPs

Segregation of CIB2 mutations, audiometric data and fundoscopy images in CIB2 patients Segregation of CIB2 mutations, audiometric data and fundoscopy images.

Re‐expression of the AXIN1 coding sequence re‐sensitizes VACO6R cells to PORCN inhibition Re‐expression of the AXIN1 coding sequence re‐sensitizes VACO6R.

Growth of TKI‐resistant EGFR delE746‐A750 PC‐9 NSCLC cells is associated with elevated cellular free fatty acid and expression of FASN, and is suppressed.

Target disease and target populations for 21st century vaccine developmentIncluded in the list are the agents of infectious diseases for which vaccines.

Treatment with AZD5363 reduces the proportion of proliferating vascular endothelial cells and following RT, the influx of CD11b+ bone marrow‐derived cells.

Etifoxine modulation of T‐cell activity during EAE

Histological and mRNA analysis of the inflammatory cytokines in the vehicle‐ or etifoxine‐treated mice at onset of clinical symptoms.A–D.At day 10 p.i.,

Nilotinib inhibits DDR1 invasive activity of patient‐derived CRC cells

In vivo silencing of Factor VII in liver hepatocytes.

LIMD1 hypoxic induction occurs via HIF‐1 and facilitates formation of a hypoxic HIF‐1 degradation complex LIMD1 hypoxic induction occurs via HIF‐1 and.

Presentation transcript:

Delivery of therapeutics to patients. Delivery of therapeutics to patients. A. Systemic delivery: (a) double stranded siRNA is packaged into delivery vehicle (targeted nanoparticles, polymers, liposomes, etc.). (b) It is then given intravenously, via inhalator or direct injection into the target tissue (the eye, tumour, etc.). B. ex‐vivo delivery: (a) cells (dendritic cells, haematopoietic stem cells, etc.) are extracted from the patient and transduced with a virus containing shRNAs. (b) The genetically modified cells are then re‐infused into the patient. Katrin Tiemann, and John J. Rossi EMBO Mol Med. 2009;1:142-151 © as stated in the article, figure or figure legend