Kerstin Bauermeister, MSc, Barbara K

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Assessment of component-resolved in vitro diagnosis of celeriac allergy Kerstin Bauermeister, MSc, Barbara K. Ballmer-Weber, MD, Merima Bublin, PhD, Philipp Fritsche, MD, Kay-Martin O. Hanschmann, MSc, Karin Hoffmann-Sommergruber, PhD, Jonas Lidholm, PhD, Christina Oberhuber, PhD, Stefanie Randow, Thomas Holzhauser, PhD, Stefan Vieths, PhD Journal of Allergy and Clinical Immunology Volume 124, Issue 6, Pages 1273-1281.e2 (December 2009) DOI: 10.1016/j.jaci.2009.07.033 Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 A, Prevalence of sensitization to different extracts and single allergens, as determined by ImmunoCAP analysis for patients with celeriac allergy (gray bars, n = 24) and control subjects with birch pollen allergy (black bars; n = 20). B, Concentration of specific IgE antibodies to tested allergens among patients with celeriac allergy (n = 24) and celeriac-tolerant atopic control subjects (n = 20). The dotted line indicates the 0.35 kUA/L cutoff point. Median values of 0.35-kUA/L or greater are indicated by horizontal bars. Numbers of negative results for each test are indicated below the cutoff line. Journal of Allergy and Clinical Immunology 2009 124, 1273-1281.e2DOI: (10.1016/j.jaci.2009.07.033) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Dose-dependent mediator release of RBL cells sensitized with sera of 10 patients with celeriac allergy. Serum from a nonallergic subject was applied as a negative control. Plots contain response curves obtained after stimulation with rBet v 1 (A), rApi g 1 (B), and nApi g 5 (C). Mediator release is expressed as a percentage of total β-hexosaminidase content determined after cell lysis. Journal of Allergy and Clinical Immunology 2009 124, 1273-1281.e2DOI: (10.1016/j.jaci.2009.07.033) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Correlation between reactivity to celeriac extract and specific reactivity to celeriac allergen components (sum of specific IgE values of all single celeriac allergens), as determined by means of ImmunoCAP analysis (R2 = 0.59). Journal of Allergy and Clinical Immunology 2009 124, 1273-1281.e2DOI: (10.1016/j.jaci.2009.07.033) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Correlation between Api g 5–specific IgE levels and IgE levels to CCDs (MUXF3 glycan of bromelain) for patients with celeriac allergy, as determined by means of ImmunoCAP analysis. IgE reactivity to nApi g 5 is slightly higher than to CCDs. The calculation of the correlation coefficient (R2 = 0.90) included all subjects with detectable specific IgE levels. Journal of Allergy and Clinical Immunology 2009 124, 1273-1281.e2DOI: (10.1016/j.jaci.2009.07.033) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions