Nishit R. Trivedi, Zhaoyuan Cong, Amanda M. Nelson, Adam J

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Presentation transcript:

Peroxisome Proliferator-Activated Receptors Increase Human Sebum Production Nishit R. Trivedi, Zhaoyuan Cong, Amanda M. Nelson, Adam J. Albert, Lorraine L. Rosamilia, Surendra Sivarajah, Kathryn L. Gilliland, Wenlei Liu, David T. Mauger, Robert A. Gabbay, Diane M. Thiboutot Journal of Investigative Dermatology Volume 126, Issue 9, Pages 2002-2009 (September 2006) DOI: 10.1038/sj.jid.5700336 Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 PPAR isoforms localize to epithelia in normal human skin. (b–d, respectively) Immunoreactivity to PPARα, δ, and γ subtypes is noted in epidermis, hair follicle, and sebaceous gland as a brown to red color. Insets demonstrate reactivity in basal cells of the sebaceous gland with expression of PPARγ extending to differentiated sebaceous cells. (a) Negative controls were treated with nonimmune serum in place of primary antibody. (a–d) Bar=200μm. Journal of Investigative Dermatology 2006 126, 2002-2009DOI: (10.1038/sj.jid.5700336) Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 PPAR subtypes are expressed in SEB-1 sebocytes. SEB-1 sebocytes were grown to 30% confluence (undifferentiated) or were treated with 1μm insulin and grown to confluence (differentiated). PPARα immunoreactivity is perinuclear under both conditions. PPARδ reactivity is perinuclear in the undifferentiated cells and nuclear in differentiated cells. PPARγ reactivity was perinuclear and cytoplasmic under both condition. Negative control sections incubated with nonimmune serum in place of primary antibody. Bar=50μm. Journal of Investigative Dermatology 2006 126, 2002-2009DOI: (10.1038/sj.jid.5700336) Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 PPAR agonists and 13-cis RA alter the pattern of lipid production in SEB-1 sebocytes. SEB-1 sebocytes were grown in SEB-1 medium until they reached 90% confluence then treated with PPARα/δ agonist GW2433 (100nm), PPARα/δ/γ agonist GW4148 (1μm), Rosiglitazone (10μm) every 48hours until the 6th day to study the differential pattern of lipid production. Cells were treated with 13-cis RA (1μm) for 72hours. Statistically significant increases were noted in the production of cholesterol and fatty acid following treatment with PPAR agonists (GW2433, GW4148, and rosiglitazone) when compared to vehicle-treated controls (P<0.01); whereas treatment with 13-cis RA showed a statistically significant decrease in the production of cholesterol, fatty alcohol, cholesterol ester, and squalene (P<0.01). Values represented are means±SE. Journal of Investigative Dermatology 2006 126, 2002-2009DOI: (10.1038/sj.jid.5700336) Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Oil Red O staining of SEB-1 sebocytes indicates decreased intracellular lipidsin SEB-1 sebocytes treated with 13-cis RA. SEB-1 sebocytes were treated with 0.1μm (a) 13-cis RA or (b) vehicle for 72hours. Note decrease in intracellular lipids with cells treated with 13-cis RA. Bar=50μm. Journal of Investigative Dermatology 2006 126, 2002-2009DOI: (10.1038/sj.jid.5700336) Copyright © 2006 The Society for Investigative Dermatology, Inc Terms and Conditions