Ex. 16: Nitrate Respiration (Nitrate Reduction Test)

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Presentation transcript:

Ex. 16: Nitrate Respiration (Nitrate Reduction Test) Objectives?

Vocabulary Oxidation – reduction reactions Reductase Nitrate vs. Nitrite Denitrification

Nitrate Reduction and Denitrification  nitrite reductase nitrate reductase Some bacteria only produce nitrate reductase, other bacteria also produce nitrite reductase

Day 1: Materials needed per table: Nitrate broth tubes (4) 1 sterile Q-tip Three pure cultures needed per table: Alcaligenes faecalis (negative) Proteus vulgaris (positive for nitrite) Pseudomonas aeruginosa (denitrifier) Each student takes responsibility to inoculate one of the nitrate broth tubes