RCPA QAP 2005 CSF Samples 9-09, 9-10 Tracks 1-4 are Isoelectric Focused (IEF) examples of QAP samples 9-09 & 9-10. Tracks 1 & 2 are stained with Coomassie.

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RCPA QAP 2005 CSF Samples 9-09, 9-10 Tracks 1-4 are Isoelectric Focused (IEF) examples of QAP samples 9-09 & 9-10. Tracks 1 & 2 are stained with Coomassie Brilliant Blue R 250 (CBB). Tracks 3 & 4 are stained with Coomassie Brilliant Blue R 250 (CBB) and then overlayed with silver stain. Application volume is 1.5μl of neat CSF. Pharmolyte Ampholytes: pH 3-10. Track 1 #9-10 Track 2 #9-09 Track 3 Track 4

RCPA QAP 2005 CSF Samples 9-09, 9-10..cont. Shown opposite are CSF QAP samples #9-09 and #9-10 which have undergone Immunoblotting onto IgG-Ab coated PVDF membrane following Isoelectric Focusing. Detection is then made with an enzyme-labelled (HRP) second antibody. Control Serum (1:101) #9-09 CSF #9-09 Serum (1:101) #9-10 CSF #9-10