Initiation of RNA Decay in Escherichia coli by 5′ Pyrophosphate Removal  Helena Celesnik, Atilio Deana, Joel G. Belasco  Molecular Cell  Volume 27, Issue 1, Pages 79-90 (July 2007) DOI: 10.1016/j.molcel.2007.05.038 Copyright © 2007 Elsevier Inc. Terms and Conditions

Figure 1 5′ End-Dependent Decay of RNA I and 5′ Extension Variants Thereof (A) RNA I and its derivative RNA I.613. The principal site of RNase E cleavage is marked with an arrow. RNA I+hp and RNA I.613+hp are identical to RNA I and RNA I.613 except for the addition of a 5′-terminal stem loop (GAUCGCCCACCGGCAGCUGCCGGUGGGCGAUC or AGAGCGGCUUCGGCCGCUCU, respectively). (B) Decay of RNA I, RNA I+hp, RNA I.613, and RNA I.613+hp. At time intervals after inhibiting transcription, total RNA was isolated from E. coli containing plasmid pRNAI, pRNAI+hp, pRNAI.613, or pRNAI.613+hp and analyzed by northern blotting. tRNACys served as an internal standard. The measured half-lives were 1.8 ± 0.1 min for RNA I, 3.5 ± 0.1 min for RNA I+hp, 0.9 ± 0.1 min for RNA I.613, and 2.8 ± 0.2 min for RNA I.613+hp. Molecular Cell 2007 27, 79-90DOI: (10.1016/j.molcel.2007.05.038) Copyright © 2007 Elsevier Inc. Terms and Conditions

Figure 2 PABLO Analysis of RNA Synthesized In Vitro (A) Method for analyzing the 5′ phosphorylation state of RNA (PABLO). T4 DNA ligase selectively joins oligo X to 5′-monophosphorylated, but not 5′-triphosphorylated, RNA annealed to the bridging oligo Y, thereby producing an extended DNA-RNA chimera detectable by northern blotting. (B) Specificity of PABLO. Internally radiolabeled RNA I.613 bearing a 5′ monophosphate or a 5′ triphosphate was prepared by in vitro transcription in the presence or absence of excess AMP. These RNAs were then subjected to PABLO with RNA I.613-specific oligonucleotides, and the effect of changing or omitting various reaction components was determined. The two different oligonucleotides X that were used, X22 (lanes 1, 2, 3, 5, and 7) and X32 (lane 4), were 22 or 32 nt long, respectively. (C) Confirmation of the 5′ phosphorylation state of in vitro transcripts. The 5′ phosphorylation state of the RNA I.613 transcripts examined in (B) was corroborated by digestion with a monophosphate-dependent 5′ exonuclease. (D) Effect of suboptimal end juxtaposition on PABLO ligation yields. The monophosphorylated RNA I.613 sample examined in (B) was subjected to PABLO with a set of bridging Y oligonucleotides that either perfectly juxtaposed the 3′ end of X32 with the 5′ end of the RNA (gap size = 0), left them separated by 1–3 nt (gap size = +1, +2, or +3), or caused them to overlap by one nucleotide (gap size = −1). Molecular Cell 2007 27, 79-90DOI: (10.1016/j.molcel.2007.05.038) Copyright © 2007 Elsevier Inc. Terms and Conditions

Figure 3 5′ Phosphorylation State of RNA I and RNA I.613 in E. coli Total RNA extracted from E. coli containing plasmid pRNAI or pRNAI.613 was subjected to PABLO analysis of RNA I or RNA I.613. As a positive control, the 5′ phosphorylation state of the fully monophosphorylated degradation intermediate RNA I-5 was also examined. Oligos X of various lengths were used (XN, where N corresponds to the length in nucleotides). In multiple experiments, the ligation yields were 9% ± 1% and 14% ± 2% for RNA I and RNA I.613, respectively. The radiolabeled probe used to detect RNA I.613 did not detect its 3′ cleavage product (RNA I-5) due to the complementarity of the probe to the 5′-terminal segment of the transcript. Molecular Cell 2007 27, 79-90DOI: (10.1016/j.molcel.2007.05.038) Copyright © 2007 Elsevier Inc. Terms and Conditions

Figure 4 Origin and Fate of Monophosphorylated RNA I.613 in E. coli (A) Accumulation of monophosphorylated RNA I.613 when RNase E cleavage is impeded. PABLO was performed to compare the 5′ phosphorylation state of RNA I.613 and RNA I.613-8C, a variant in which the primary RNase E cleavage site of RNA I.613 had been mutated to impede cleavage (ACAGUAUUU → ACCCCCCCC; see Figure 1A). In multiple experiments in E. coli, the ligation yield consistently doubled when RNase E cleavage was inhibited. (B) Phosphorylation state of the 38 nt 5′-terminal decay intermediate produced in E. coli by RNase E cleavage of RNA I.613 (left) or in vitro by RNase E cleavage of fully monophosphorylated, internally radiolabeled RNA I.613 (right). In multiple experiments in E. coli, the PABLO ligation yields were 14% ± 2% and 55% ± 7% for full-length RNA I.613 and its 5′ cleavage product, respectively. (C) Invariant ratio of monophosphorylated to triphosphorylated RNA I.613 after transcription inhibition. (Left) PABLO analysis of RNA I.613 isolated from E. coli at time intervals after inhibiting transcription. (Right) Concentration of monophosphorylated RNA I.613 (ligation product) and triphosphorylated RNA I.613 (unligated) as a function of time after transcription inhibition. Molecular Cell 2007 27, 79-90DOI: (10.1016/j.molcel.2007.05.038) Copyright © 2007 Elsevier Inc. Terms and Conditions

Figure 5 Phosphorylation State of the rpsT P1 Transcript and Its HO-rpsT Counterpart in E. coli (A) 5′ phosphorylation state of rpsT mRNA. (Left) The E. coli rpsT transcriptional unit. Jagged lines ending in arrowheads, primary transcripts. Gray rectangle, protein-coding region. (Right) PABLO analysis of the chromosomal rpsT P1 transcript in E. coli. In multiple experiments, the ligation yield was 25% ± 2% for the rpsT P1 transcript. (B) Generation of HO-rpsT mRNA by self-cleavage of the hammerhead-containing rpsT.HH transcript. Only the 5′-terminal segment of the transcript is shown. (C) 5′ phosphorylation state of HO-rpsT mRNA. (Left) The rpsT.HH transcriptional unit. (Right) PABLO analysis of HO-rpsT mRNA in E. coli. The plasmid-encoded rpsT P1 transcript and its self-cleaved HO-rpsT P1 counterpart were detected with a radiolabeled probe complementary to a sequence tag inserted into the 3′UTR. Molecular Cell 2007 27, 79-90DOI: (10.1016/j.molcel.2007.05.038) Copyright © 2007 Elsevier Inc. Terms and Conditions

Figure 6 Stabilization of rpsT mRNA in E. coli by a 5′ Hydroxyl (A) Decay of HO-rpsT mRNA in E. coli. At time intervals after inhibiting transcription, total RNA was isolated from E. coli containing plasmid pRPST1 (top left) or pRPST.HH (bottom left) and analyzed by northern blotting with a radiolabeled probe complementary to a 3′UTR sequence tag. Degradation rates were calculated for rpsT P1 mRNA bearing a 5′-terminal hydroxyl (HO-rpsT; half-life = 5.3 ± 0.1 min) or a 5′-terminal triphosphate (TriP-rpsT; half-life = 0.9 ± 0.1 min) by plotting band intensity as a function of time (right). In each case, the triphosphorylated P2 transcript (internal standard) decayed with a half-life of 1.2 ± 0.3 min. (B) Equivalent RNase E cleavage rates in vitro for rpsT RNAs bearing a 5′ triphosphate or a 5′ hydroxyl. Equal amounts of radiolabeled, in vitro-synthesized rpsT RNA bearing a 5′ monophosphate, 5′ triphosphate, or 5′ hydroxyl were treated with purified N-RNase E under identical conditions, and samples quenched at time intervals were analyzed by gel electrophoresis and autoradiography. Molecular Cell 2007 27, 79-90DOI: (10.1016/j.molcel.2007.05.038) Copyright © 2007 Elsevier Inc. Terms and Conditions

Figure 7 Mechanism of the 5′ End-Dependent Pathway for RNA Degradation in E. coli An RNA pyrophosphohydrolase (hatchet) removes the γ and β phosphates at the 5′ terminus of a triphosphorylated primary transcript, either simultaneously (as shown) or sequentially. The resulting monophosphorylated decay intermediate is then rapidly cleaved by RNase E (scissors linked to a 5′-sensor domain), an endonuclease with a marked preference for RNA substrates bearing a single phosphate group at the 5′ end. Molecular Cell 2007 27, 79-90DOI: (10.1016/j.molcel.2007.05.038) Copyright © 2007 Elsevier Inc. Terms and Conditions