Amplified fragment length polymorphism analysis in the evaluation of posttransfusion graft-versus-host disease in a child undergoing extracorporeal membrane.

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Presentation transcript:

Amplified fragment length polymorphism analysis in the evaluation of posttransfusion graft-versus-host disease in a child undergoing extracorporeal membrane oxygenation Louis DePalma, MD, K.Rais Bahrami, MD, Sudesh Kapur, MD, Billie L. Short, MD, Naomi L.C. Luban, MD The Journal of Thoracic and Cardiovascular Surgery Volume 108, Issue 1, Pages 182-184 (July 1994) DOI: 10.5555/uri:pii:S0022522394702403 Copyright © 1994 Mosby, Inc. Terms and Conditions

Fig. 1 Skin biopsy. The epidermis shows a lymphocytic infiltrate with single cell necrosis and basal cell vacuolar degeneration. The papillary dermis is infiltrated by a mononuclear cell population. The Journal of Thoracic and Cardiovascular Surgery 1994 108, 182-184DOI: (10.5555/uri:pii:S0022522394702403) Copyright © 1994 Mosby, Inc. Terms and Conditions

Fig. 2 FLP analysis of the Col2A1 region. Lane 1, Father's mononuclear cells; lane 2, mother's mononuclear cells; lane 3, patient's mononuclear cells; lane 4, patient's neutrophils; lane 5, patient's skin; lane 6, laboratory standard for sensitivity at 5%; lane 7, laboratory standard for sensitivity at 1%; lane 8, negative control (no DNA). The Journal of Thoracic and Cardiovascular Surgery 1994 108, 182-184DOI: (10.5555/uri:pii:S0022522394702403) Copyright © 1994 Mosby, Inc. Terms and Conditions