STARD3‐mediated cholesterol accumulation in endosomes occurs at the expense of plasma membrane STARD3‐mediated cholesterol accumulation in endosomes occurs.

Slides:

Advertisements

Similar presentations

Glioma-derived soluble factor(s) enhance IL-10 signaling.

Advertisements

Validating Cdk‐dependent chromatin association changes of selected outliers A–EValidation of changes in chromatin affinity following Cdk inhibition of.

Loss of USP8 or HIF1α impairs endocytic recycling required for ciliogenesis Kinetic analysis of transferrin recycling measured by flow cytometry. Loss.

Induction of apoptosis by CDIP expression.

MOSPD2 recruitment to interorganelle contact sites depends on the interaction between the FFAT motif and the MSP domain MOSPD2 recruitment to interorganelle.

Induction of DNA double‐strand breaks and activation of the ataxia telangiectasia mutated pathway in post‐mitotic cells in response to camptothecin. Induction.

Overexpression of RNase H1 decreases the induction of phosphorylated H2AX in post‐mitotic cells in response to topoisomerase 1 cleavage complexes. Overexpression.

Inhibition of SERCA by cyclopiazonic acid normalises both the early decrease and late increase in cytosolic calcium and enhances the viability in differentiated.

AβOs bind to and impact hypothalamic neurons Representative immunocytochemistry images of mature hypothalamic neurons in culture exposed to vehicle (Veh)

Volume 15, Issue 1, Pages (January 2014)

Palbociclib activates proteasomal protein degradation

Endogenous MOSPD2 is recruited to interorganelle contact sites by FFAT‐containing proteins Endogenous MOSPD2 is recruited to interorganelle contact sites.

VAP‐A is recruited to interorganelle contact sites together with MOSPD2 VAP‐A is recruited to interorganelle contact sites together with MOSPD2 AHeLa cells.

An Essential Role of Hrs/Vps27 in Endosomal Cholesterol Trafficking

Fig. 4. aPD-1 mAb transfer to macrophages is mediated by FcγRs.

Enhancement of basal mitophagy by USP30 depletion is dependent on PINK1 Enhancement of basal mitophagy by USP30 depletion is dependent on PINK1 Representative.

Chd5 deficiency in NSCs leads to premature activation.

ECM 29 and Kaplan–Meier survival curves for additional datasets

ADAM8 promotes cancer cell adhesion to endothelial cells via β1‐integrin activation ABlood was drawn using the submandibular collection method from 4 mice/group.

Palbociclib activates the proteasome indirectly and reduces the association of ECM29 with the proteasome Palbociclib activates the proteasome indirectly.

Insulin regulates the expression and intra-membrane proteolysis of the recombinant HA-pSREBP-1c-Flag protein in McA-RH7777 cells Insulin regulates the.

HPV‐E7/RB axis regulates ceramide‐dependent mitophagy via E2F5 activation HPV‐E7/RB axis regulates ceramide‐dependent mitophagy via E2F5 activation AUM‐SCC‐47.

KLK7 is involved in the Aβ degradation activity

USP30 restricts basal pexophagy

PEX13 is required for Sindbis virophagy but not general autophagy

Beclin‐1 depletion reduces targeting of several outer kinetochore proteins. Beclin‐1 depletion reduces targeting of several outer kinetochore proteins.

Endogenous MOSPD2 is recruited to interorganelle contact sites by FFAT‐containing proteins Endogenous MOSPD2 is recruited to interorganelle contact sites.

Selection and Identification of Skeletal-Muscle-Targeted RNA Aptamers

Nec‐1 inhibits the phosphorylation and aggregation of tau

Characterization of cholesterol‐enriched endosomes in HeLa/STARD3 cells Characterization of cholesterol‐enriched endosomes in HeLa/STARD3 cells HeLa/STARD3.

STARD3 favors cholesterol accumulation in endosomes

The ER is the main source of cholesterol accumulated by STARD3 in endosomes The ER is the main source of cholesterol accumulated by STARD3 in endosomes.

MOSPD2 silencing affects interorganelle contact sites organization

VAP silencing restores a normal plasma membrane cholesterol level in STARD3‐expressing cells VAP silencing restores a normal plasma membrane cholesterol.

VAP protein knockdown abolishes STARD3‐mediated cholesterol accumulation in endosomes VAP protein knockdown abolishes STARD3‐mediated cholesterol accumulation.

ER stress response and susceptibility to apoptosis are regulated by TFEB and TFE3 ER stress response and susceptibility to apoptosis are regulated by TFEB.

Fmrp regulates E‐cadherin and Vimentin in breast cancer cells Fmrp (red) and E‐cadherin (green) detection by I.F. in 4T1 cells expressing different Fmrp.

A1 A2 A3 A4 Figure S1. (A2, C2) hPDLSCs at P2 show low expression of p16 and p21 (red fluorescent) as indicated by double immunostaining. (A3, C3) Cells.

EnAd‐EpCAMBiTE shows reproducible activity within an expanded cohort of patient malignant peritoneal and pleural exudates EnAd‐EpCAMBiTE shows reproducible.

Apoptosis and necroptosis of L929 cells results in the release of mitochondria. Apoptosis and necroptosis of L929 cells results in the release of mitochondria.

Enhancement of receptor endocytosis, as well as cellular apoptosis, may explain the cooperative anti‐tumor actions of 3×mAbs and osimertinib Enhancement.

Volume 21, Issue 11, Pages (November 2014)

HPV‐E7 targets RB for induction of ceramide‐dependent mitophagy

Vimentin is dispensable for multiple B cell–intrinsic processes.

R1441G‐LRRK2 co‐localizes with Rab10 in the periphery and Rab8 near the nucleus R1441G‐LRRK2 co‐localizes with Rab10 in the periphery and Rab8 near the.

STARD3‐mediated cholesterol accumulation in endosomes does not alter cholesterol homeostasis STARD3‐mediated cholesterol accumulation in endosomes does.

Palbociclib increases proteasome activity and the clearance of protein aggregates without significant effects on autophagy Palbociclib increases proteasome.

ZBTB48 is required for MTFP1 expression

VAP protein knockdown abolishes STARD3‐mediated cholesterol accumulation in endosomes VAP protein knockdown abolishes STARD3‐mediated cholesterol accumulation.

Impact of ONX 0914‐induced neutrophilia during CVB3 infection

AS aggregates interact with SERCA in SH‐SY5Y cells

Absence of miR‐21 in macrophages promotes foam cell formation

Changes in fibroblast activation and proliferation outside the wound and CHP staining in the wound bed (related to Fig 5)‏ Changes in fibroblast activation.

The GBS hemolytic pigment/lipid toxin induces caspase 1 activation and pyroptosis THP‐1 macrophages were treated with GBS pigment, and caspase 1 activation.

FGF/ERK inhibition accelerates neural fate conversion of EpiSCs.

High-affinity binding to the hypervariable region of the β1 I-like domain controls signaling to fibronectin fibrillogenesis. High-affinity binding to the.

Figure 4 DNM1 mutations affect protein levels and self-dimerization (A) HeLa cells were transfected with green fluorescent protein (GFP)-tagged mutant.

Ventral mouse V-SVZ cells exhibit higher mTORC1 activity than dorsal cells and are responsive to rapamycin. Ventral mouse V-SVZ cells exhibit higher mTORC1.

TOR, but not OSP, blocks STx2B transport and alters endosomal dynamics

High-affinity mutants of β3 integrin fail to stimulate RhoA activity and fibronectin fibrillogenesis. High-affinity mutants of β3 integrin fail to stimulate.

Effect of a Fusion Peptide by Covalent Conjugation of a Mitochondrial Cell-Penetrating Peptide and a Glutathione Analog Peptide Carmine Pasquale Cerrato,

Arginine-to-lysine mutations conferring TRIM22 restriction and lysine-to-arginine mutations causing loss of TRIM22 restriction. Arginine-to-lysine mutations.

Fluorescence flow cytometry shows no difference in total protein levels but reveals a slight difference in cell size. Fluorescence flow cytometry shows.

AAS-dependent induction of autophagic flux in ARPE-19.

Integrin β3 is dispensable for RhoA activity and contractility.

Aβ-mediated Ras-MAPK signaling and Cyclin D1 expression in B103 cells are dependent on APP expression and can be reversed with MEK or Ras inhibition. Aβ-mediated.

The role of p53 in H2O2-mediated cell death of hOGG1-deficient H1299 lung carcinoma p53 null cells. The role of p53 in H2O2-mediated cell death of hOGG1-deficient.

Volume 14, Issue 4, Pages (February 2016)

Fig. 5 C9orf72 knockdown disrupts autophagy induction.

AS1411 alters subcellular distribution of PRMT5 in a time-dependent, dose-dependent, and nucleolin-dependent manner. AS1411 alters subcellular distribution.

Presentation transcript:

STARD3‐mediated cholesterol accumulation in endosomes occurs at the expense of plasma membrane STARD3‐mediated cholesterol accumulation in endosomes occurs at the expense of plasma membrane Indirect analysis of plasma membrane cholesterol content. Cells were treated with amphotericin B (20 μg/ml; 4 h at 37°C), and cell viability was measured using a luminescent cell viability assay. Relative cell viability is represented on the graph. Mean ± SD; n: number of independent experiments; HeLa, HeLa/Ctrl, HeLa/STARD3: n = 5; HeLa/STARD3 ΔSTART; HeLa/STARD3 MR/ND; HeLa/STARD3 FA/YA: n = 4. **P < 0.01, ***P < 0.001, ANOVA with Tukey's multiple comparison test.Plasma membrane cholesterol labeling using the GFP‐D4 probe. Live cells were incubated with GFP‐D4 probe (green) prior to fixation and nuclei staining (blue). Both signals are merged (a–f). Scale bar: 10 μm. (g) Quantification of mCherry‐D4 membrane labeling by flow cytometry. Fluorescence mean intensities are compared with that of control cells set to one. Mean ± SD; n = 7 independent experiments; paired two‐tailed t‐test; ***P = 0.0006.Western blot analysis (a) of STARD3 in HeLa cells, HeLa cells expressing a control shRNA (shCtrl) and in HeLa cells expressing two shRNAs targeting STARD3 (shSTARD3‐α and shSTARD3‐β). Actin was used as a loading control. Plasma membrane cholesterol was labeled using the GFP‐D4 probe (b) under normal cell culture conditions and under LPDS culture conditions (24‐h treatment); flow cytometry quantification of the GFP‐D4 staining is expressed as mean intensity with that of control cell in normal medium set to one. Mean ± SD; n = 9 independent experiments; **P < 0.01, ***P < 0.001, ANOVA with Tukey's multiple comparison test. Léa P Wilhelm et al. EMBO J. 2017;36:1412-1433 © as stated in the article, figure or figure legend