EnAd‐EpCAMBiTE shows reproducible activity within an expanded cohort of patient malignant peritoneal and pleural exudates EnAd‐EpCAMBiTE shows reproducible.

Slides:

Advertisements

Similar presentations

Expression of follicular cell‐derived VEGF‐A and endothelial VEGFR is altered by different circulating TSH level Expression of follicular cell‐derived.

Advertisements

Effects of inhibiting inflammatory cell recruitment on fracture healing Treatment with CCR2 antagonist, INCB3344, led to impaired fracture healing compared.

Impact of triple combination of gemcitabine‐based chemoirradiation with anti‐PD‐L1 in the KPC tumor allograft Impact of triple combination of gemcitabine‐based.

Reduced scar formation in the injured GM of CCR2−/− mice

Metabolic effects of TUG‐891 are reduced or absent in GPR120‐deficient mice Metabolic effects of TUG‐891 are reduced or absent in GPR120‐deficient mice.

HDAC3 regulates AKT phosphorylation

The HDAC3 inhibitor suppresses SPOP‐mutated prostate cancer cell growth The HDAC3 inhibitor suppresses SPOP‐mutated prostate cancer cell growth A22Rv1.

Neuro‐inflammatory cytokines, brain microglial activation, and working memory deficits are normalized using LV.IDS.ApoEII, but not LV.IDS in MPS II mice.

PbA‐specific CD4 T‐cell responses are maximal at day 6 post‐pRBC infection PbA‐specific CD4 T‐cell responses are maximal at day 6 post‐pRBC infection Proportion.

Acetylcholine/muscarinic signaling maintains SOX2 and the acinar lineage in human SG Acetylcholine/muscarinic signaling maintains SOX2 and the acinar lineage.

[68Ga]Pentixafor PET imaging of MM xenografts Real‐time PCR analysis of cxcr4 transcript expression levels in HeLa (negative control) and in MM cell lines.

AAV8‐hAAT‐FGF21 treatment improves liver fibrosis

Differential accumulation of substrates in vivo

Treatment with FGF21‐encoding vectors reverses hepatic steatosis and fibrosis Treatment with FGF21‐encoding vectors reverses hepatic steatosis and fibrosis.

Fstl1 promotes cardiac fibroblasts proliferation via the ERK1/2‐dependent pathway Fstl1 promotes cardiac fibroblasts proliferation via the ERK1/2‐dependent.

TGF‐β1 and Sema3A are downregulated in vivo by increasing VEGF doses Muscles were harvested 7 days after implantation of V Low, V Med, and V High clones.

Chd5 deficiency in NSCs leads to premature activation.

PVSRIPO-mediated APC activation occurs in immunosuppressive conditions

Increased IFNγ producing CD4+ T cells in the spleen drives the enhanced splenic IFNγ on 6 dpi during concurrent co‐infection Increased IFNγ producing CD4+

No neurodegenerative phenotype observed in single Aph1a and Aph1bc cKO Cre+ mice No neurodegenerative phenotype observed in single Aph1a and Aph1bc cKO.

SPP1 and TNC are induced by active JNK and promote lung metastasis

Expression of PD‐1 on PBMC and malignant exudate T cells

Integrated protein co‐expression and differential expression across the ALS‐FTD disease continuum Integrated protein co‐expression and differential expression.

Superior potency of EnAd expressing EpCAM BiTE in partially EnAd‐resistant cancer cell line Superior potency of EnAd expressing EpCAM BiTE in partially.

SPRY2 deficiency‐induced IL6 drives CRPC by elevating tumoral HSD3B1 and cholesterol levels SPRY2 deficiency‐induced IL6 drives CRPC by elevating tumoral.

PERK activator treatment protects neurite network

Inhibition of CDK6 kinase activity sensitizes EOC cells to platinum in vitro Inhibition of CDK6 kinase activity sensitizes EOC cells to platinum in vitro.

Neuro‐inflammatory cytokines, brain microglial activation, and working memory deficits are normalized using LV.IDS.ApoEII, but not LV.IDS in MPS II mice.

Treatment with FGF21‐encoding vectors reverses hepatic steatosis and fibrosis Treatment with FGF21‐encoding vectors reverses hepatic steatosis and fibrosis.

High frequency and unique tumor‐initiating cells (TICs) in Pten/p53‐deficient mice Flow cytometry profiles of indicated tumors with the CD24 and CD49f.

Impaired ECM protein synthesis in infarcted area of S100a4cre+/− × Fstl1flox/flox mice Impaired ECM protein synthesis in infarcted area of S100a4cre+/−

IR induces cellular damage and a loss of nerves and SOX2 and SOX2+ progenitors can replenish mouse acinar cells in response to cholinergic mimetics IR.

STARD3 favors cholesterol accumulation in endosomes

The ER is the main source of cholesterol accumulated by STARD3 in endosomes The ER is the main source of cholesterol accumulated by STARD3 in endosomes.

TGF‐β1 treatment induces the chromatin binding of Smad2/3/4 in 4T1 cells TGF‐β1 treatment induces the chromatin binding of Smad2/3/4 in 4T1 cells A, BWestern.

VAP silencing restores a normal plasma membrane cholesterol level in STARD3‐expressing cells VAP silencing restores a normal plasma membrane cholesterol.

Normal priming and expansion of functional T cells in the spleen during concurrent co‐infection Normal priming and expansion of functional T cells in the.

ER stress response and susceptibility to apoptosis are regulated by TFEB and TFE3 ER stress response and susceptibility to apoptosis are regulated by TFEB.

IL‐23‐induced psoriasis‐like skin disease is ameliorated in IL‐23−/− mice IL‐23‐induced psoriasis‐like skin disease is ameliorated in IL‐23−/− mice ARepresentative.

Cochlear explant and slice preparation, and CDDP‐induced DNA damage and cell death in sensory hair cells and spiral ganglion neurons Cochlear explant and.

Safety profile of reversibly immortalised human mesoangioblasts

Impact of ONX 0914 on abundance and dissemination of monocytes/macrophages during CVB3 infection Impact of ONX 0914 on abundance and dissemination of monocytes/macrophages.

TGFβ signaling activity in smooth muscle cells in human left main coronary arteries with various degrees of atherosclerosis TGFβ signaling activity in.

STARD3‐mediated cholesterol accumulation in endosomes occurs at the expense of plasma membrane STARD3‐mediated cholesterol accumulation in endosomes occurs.

Proteasomal inhibition suppresses palbociclib‐induced senescence phenotype Proteasomal inhibition suppresses palbociclib‐induced senescence phenotype ARepresentative.

Enhancement of receptor endocytosis, as well as cellular apoptosis, may explain the cooperative anti‐tumor actions of 3×mAbs and osimertinib Enhancement.

miR‐34a‐5p acts in PDGFRα+ cells to block lung alveolarization

MiR‐34a‐5p functionally contributes to arrested lung alveolarization in response to hyperoxia miR‐34a‐5p functionally contributes to arrested lung alveolarization.

Both the number and the OX40L expression level of bronchiolar progenitors were increased by influenza infection. Wild‐type mice were intratracheally infected.

The CHCH domain is necessary for mitochondrial import of CHCHD10

High CDK6 expression predicts poor prognosis in EOC patients

Treatment with AZD5363 reduces the proportion of proliferating vascular endothelial cells and following RT, the influx of CD11b+ bone marrow‐derived cells.

Effect of RK‐33 on radiation‐induced DNA damage AImmunofluorescence images showing 53BP1 and γH2AX foci in A549 cells after 2‐Gy radiation and A549 cells.

Etifoxine modulation of T‐cell activity during EAE

Therapeutic activity of gal‐encapsulated cytotoxic drugs on tumor xenografts Therapeutic activity of gal‐encapsulated cytotoxic drugs on tumor xenografts.

Tissue‐specific differences in the levels of CoQ9, CoQ10, and DMQ9, and in the DMQ9/CoQ9 ratio after β‐RA treatment in Coq9R239X mice Tissue‐specific differences.

Sox2 is essential for SLG regeneration following radiation injury

Antagonizing miR‐34a function partially restores proper lung alveolarization Antagonizing miR‐34a function partially restores proper lung alveolarization.

Effect of D‐Asp treatment on myelination and remyelination in cerebellar organotypic slices Effect of D‐Asp treatment on myelination and remyelination.

GM1 decreases neuropathology and weight loss in R6/2 mice

AS aggregates interact with SERCA in SH‐SY5Y cells

AOX modifies RISP assembly and rescues mitochondrial respiration in tissue‐specific fashion AOX modifies RISP assembly and rescues mitochondrial respiration.

Symptomatic rescue of double‐transgenic mice without decreased protein aggregation The expression of exogenous HMGB1‐FLAG (detected with an anti‐FLAG antibody)

GDF11 induces myotube atrophy in vitro

CAR T‐cell generation in human PBMC‐transplanted mice

UCN‐01 inhibits human IPF lung fibroblast pathological phenotypes and attenuates lung fibrosis induced by bleomycin injury UCN‐01 inhibits human IPF lung.

Absence of miR‐21 in macrophages promotes foam cell formation

Bazedoxifene suppresses IL11‐mediated STAT3 signaling activity in human cancer cell lines and patient‐derived colon cancer primary cultures and organoids.

SEMA3C regulates prostate cancer cell growth

Malaria inflammation by xanthine oxidase‐produced reactive oxygen species A, BMacrophages produce little to no inflammatory cytokines when incubated with.

Presentation transcript:

EnAd‐EpCAMBiTE shows reproducible activity within an expanded cohort of patient malignant peritoneal and pleural exudates EnAd‐EpCAMBiTE shows reproducible activity within an expanded cohort of patient malignant peritoneal and pleural exudates A–CUnpurified total cells from ascites or pleural effusions (from seven different patients; pleural effusion, blue; peritoneal ascites, red) were incubated in 50% fluid from the same exudate sample in the presence of free BiTE, EnAd or recombinant virus. After 5 days, the total cell population was harvested, and the number of (A) CD3+ T cells and those which were (B) CD25+ was quantified. (C) The number of EpCAM+ cells was measured using flow cytometry.DRepresentative microscopy images (magnification ×10; scale bar, 100 μm) and flow cytometry analysis of pleural effusion cells of patient 3 (cancer cells and lymphocytes) following treatment with EnAd or EnAd‐SA‐EpCAM BiTE. Pink, total cells; blue, CD3+ cells; red, CD3+CD25+ cells; black, EpCAM+ cells.EAt 5 days, cytokine levels were measured by LEGENDplex human Th cytokine panel using pleural effusion cultures following incubation with free recombinant BiTE or infection with EnAd or recombinant virus. E:T (CD3+:EpCAM+) ratio upon receipt of patient samples were 0.51 (patient 1), 37.6 (patient 2), 0.39 (patient 3), 4.44 (patient 4), 0.14 (patient 5), 6.12 (patient 6) and 30.6 (patient 7).Data information: Each condition was measured in biological triplicate and represented as mean ± SD. Significance was assessed by comparison to untreated control samples using a one‐way ANOVA test with Tukey's post hoc analysis, *P < 0.05, **P < 0.01, ***P < 0.001. Source data are available online for this figure. Joshua D Freedman et al. EMBO Mol Med. 2017;emmm.201707567 © as stated in the article, figure or figure legend