The effects of solution temperature on catalase reaction rates Mariah Hamilton, Gracie Hobbs, Rose Millay, Annie Miller

PICOT During catalyzation, does increased temperature or room temperature raise the rate of reaction during the reaction over 200 seconds (0°C, 21°C, 50°C, 55°C)?

Conduction of Experiment Place 6mL of H2O in each flask and test tube. Add 6mL of 3% H2O2 in each flask and test tube. Place one tube in a 0°C ice bath, both flasks on a 50°C hot plate, and leave one tube at room temperature (21°C). Place 2 drops of catalase into room temperature test tube, place stopper in test tube, and measure reaction on LoggerPro for 200 seconds. Once the other test tube and flasks have reached desired temperatures, repeat the process used on the room temperature tube for each. For both of the heated flasks, place the stirrers in the solutions and turn on the aggravators. Record the slope of each line.

Analysis of Results The 0°C solution had a slope of 0.05834 kPa/s. We concluded that the solution cooled to 0°C had the steepest slope, beating 21°C, 50°C, and 55°C, which makes it most effective at maximizing catalase reactions.