Putative stem cell activity of human endometrial epithelial and stromal cells during the menstrual cycle  Kjiana Elkje Schwab, B.Biomed.Sci. (Hons.),

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Putative stem cell activity of human endometrial epithelial and stromal cells during the menstrual cycle  Kjiana Elkje Schwab, B.Biomed.Sci. (Hons.), Rachel Wah Shan Chan, B.Sci. (Hons.), Caroline Eve Gargett, Ph.D.  Fertility and Sterility  Volume 84, Pages 1124-1130 (October 2005) DOI: 10.1016/j.fertnstert.2005.02.056 Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 1 Cloning efficiency of human endometrial epithelial (A) and stromal (B) cells from proliferative, secretory, and inactive endometrium cultured in serum-containing medium. Each bar consists of the cloning efficiency for small (solid bars) and large (white bars) colonies, which together represent the total cloning efficiency observed. Results shown are means ± SEM for the number of samples shown below the bars. Not all samples were examined for both cell types in serum-containing medium. Schwab. Endometrial stem cells and menstrual cycle. Fertil Steril 2005. Fertility and Sterility 2005 84, 1124-1130DOI: (10.1016/j.fertnstert.2005.02.056) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 2 Cloning efficiency of human endometrial epithelial cells for proliferative, secretory, and inactive endometrium cultured in serum-free medium on feeder layers in the presence of individual growth factors. The dashed line represents the total basal cloning efficiency (n = 7) for epithelial cells cultured on feeder layer in the absence of any growth factor. Results shown are means ± SEM for proliferative (n = 5–7), secretory (n = 5–6), and inactive (n = 2–4) endometrium. Not all growth factors were assessed for each sample. *Statistical significance between the three endometrial groups for small colonies; P<.05. Schwab. Endometrial stem cells and menstrual cycle. Fertil Steril 2005. Fertility and Sterility 2005 84, 1124-1130DOI: (10.1016/j.fertnstert.2005.02.056) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 3 Cloning efficiency of human endometrial stromal cells for proliferative, secretory, and inactive endometrium cultured in SFM in the presence of individual growth factors. Only those growth factors that supported stromal cell clonogenicity are shown. Results shown are means ± SEM for proliferative (n = 5), secretory (n = 5–7), and inactive (n = 3–4) endometrium. Not all growth factors were assessed for each sample. Statistical significance between the three endometrial groups for total clonogenicity (†P<.01), large colonies (**P<.05; ‡P<.01), and small colonies (*P<.05). Schwab. Endometrial stem cells and menstrual cycle. Fertil Steril 2005. Fertility and Sterility 2005 84, 1124-1130DOI: (10.1016/j.fertnstert.2005.02.056) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions