Bita Nickkholgh, M. D. , Sefika Canan Mizrak, V. M. D. , Ph. D

Slides:

Advertisements

Similar presentations

Mouse strain and quality control testing: improved sensitivity of the mouse embryo assay with embryos from outbred mice Zaraq Khan, M.B.B.S., Heather S.

Advertisements

Integration of immunodeficiency virus in oocytes via intracytoplasmic injection: possible but extremely unlikely Marjan M.C. Steenvoorden, Ph.D., Marion.

Yong-An Lee, Ph. D. , Yong-Hee Kim, B. S. , Seung-Jung Ha, B. S

Leukocytes coincubated with human sperm trigger classic neutrophil extracellular traps formation, reducing sperm motility Fabiola Zambrano, M.Sc., Tessa.

Volume 5, Issue 1, Pages (July 2015)

Identification of human spermatogenesis-related proteins by comparative proteomic analysis: a preliminary study Ran Huo, Ph.D., Ying He, B.M., Chun Zhao,

Differential expression of G-protein-coupled estrogen receptor-30 in human myometrial and uterine leiomyoma smooth muscle Ruijuan Tian, M.Sc., Zengyong.

Human spermatogonial stem cells display limited proliferation in vitro under mouse spermatogonial stem cell culture conditions Jose V. Medrano, Ph.D.,

Sefika Canan Mizrak, Federica M.F. van Dissel-Emiliani

MicroRNA expression in the human blastocyst

Lapatinib inhibits meiotic maturation of porcine oocyte-cumulus complexes cultured in vitro in gonadotropin-supplemented medium Eva Nagyova, D.V.M.,

Inhibition of mammalian target of rapamycin signaling pathway decreases retinoic acid stimulated gene 8 expression in adult mouse testis Pinar Sahin,

Volume 2, Issue 4, Pages (April 2008)

Bone marrow stem cells transplanted to the testis of sterile mice do not differentiate into spermatogonial stem cells and have no protective effect on.

Contribution of different bone marrow-derived cell types in endometrial regeneration using an irradiated murine model Claudia Gil-Sanchis, Ph.D., Irene.

Comparative gene expression profiling of adult mouse ovary-derived oogonial stem cells supports a distinct cellular identity Anthony N. Imudia, M.D.,

Vitrification and xenografting of human ovarian tissue

Composition of single-step media used for human embryo culture

Patrizia Carrarelli, Ph. D. , Chih-Fen Yen, M. D. , Felice Arcuri, Ph

Regeneration of spermatogenesis by grafting testicular tissue or injecting testicular cells into the testes of sterile mice: a comparative study Dorien.

Dorien Van Saen, Ph. D. , Ellen Goossens, Ph. D. , Joeri L. Aerts, Ph

Comparative evaluation of different in vitro systems that stimulate germ cell differentiation in human embryonic stem cells Mark Richards, Ph.D., Chui-Yee.

Spermatogonial survival in long-term human prepubertal xenografts

Bone morphogenetic protein 4 promotes mammalian oogonial stem cell differentiation via Smad1/5/8 signaling Eun-Sil Park, Ph.D., Dori C. Woods, Ph.D.,

Min Li, M. Sc. , Dong Liu, M. Sc. , Li Wang, Ph. D. , Weizhou Wang, B

Computer-assisted motility analysis of spermatozoa obtained after spermatogonial stem cell transplantation in the mouse Ellen Goossens, Ph.D., Gert De.

Ying-Hung Lin, Ph. D. , Chuan-Kai Chou, M. Sc. , Yu-Ching Hung, M. Sc

Gene and protein expression signature of endometrial glandular and stromal compartments during the window of implantation Gloria E. Evans, Ph.D., José.

Multidrug-resistant transport activity protects oocytes from chemotherapeutic agents and changes during oocyte maturation Lynae M. Brayboy, M.D., Nathalie.

Age-related changes in the localization of DNA methyltransferases during meiotic maturation in mouse oocytes Lu Zhang, M.Sc., Dan-Yu Lu, M.Sc., Wan-Yun.

Yang Li, Ph.D., Ji-chun Tan, M.D., Ph.D., Ling-song Li, M.D., Ph.D.

Genetic variants in the ETV5 gene in fertile and infertile men with nonobstructive azoospermia associated with Sertoli cell–only syndrome Moira K. O'Bryan,

Proteomic analysis of individual human embryos to identify novel biomarkers of development and viability Mandy G. Katz-Jaffe, Ph.D., David K. Gardner,

Aquaporin 7 expression in postimplantation mouse uteri: a potential role for glycerol transport in uterine decidualization Hongying Peng, M.D., Ying.

Ramadan Saleh, M. D. , Reda Z. Mahfouz, M. D. , Ashok Agarwal, Ph. D

Estrogenic regulation of testicular expression of stem cell factor and c-kit: implications in germ cell survival and male fertility Sara Correia, M.S.,

Volume 10, Issue 2, Pages (February 2018)

Acetylated α-tubulin is reduced in individuals with poor sperm motility Shweta Bhagwat, M.Sc., Veena Dalvi, M.Sc., Darshan Chandrasekhar, M.Sc., Tinsu.

Reply of the Authors Fertility and Sterility

Gadolinium radiologic contrast is a useful alternative for hysterosalpingography in patients with iodine allergy Dorette Noorhasan, M.D., Michael J.

HOXA-10 and E-cadherin expression in the endometrium of women with recurrent implantation failure and recurrent miscarriage Yihua Yang, Ph.D., Xiaoyan.

Status of nucleolar channel systems in uterine secretions accurately reflects their prevalence—a marker for the window of implantation—in simultaneously.

Integrative DNA methylation and gene expression analysis identifies discoidin domain receptor 1 association with idiopathic nonobstructive azoospermia

Min Li, M. Sc. , Dong Liu, M. Sc. , Li Wang, Ph. D. , Weizhou Wang, B

Identification of molecular markers for endometriosis in blood lymphocytes by using deoxyribonucleic acid microarrays Idhaliz Flores, Ph.D., Elizabeth.

Testicular Architecture Is Critical for Mediation of Retinoic Acid Responsiveness by Undifferentiated Spermatogonial Subtypes in the Mouse Tessa Lord,

Presence of spermatogonia in 47,XXY men with no spermatozoa recovered after testicular sperm extraction Dorien Van Saen, M.Sc., Herman Tournaye, M.D.,

Germline stem cells: toward the regeneration of spermatogenesis

Suppression of spermatogenesis before grafting increases survival and supports resurgence of spermatogenesis in adult mouse testis Lucía Arregui, Ph.D.,

High concentration of synthetic serum, stepwise equilibration and slow cooling as an efficient technique for large-scale cryopreservation of human embryonic.

Florian Schneider, M. D. , Klaus Redmann, Ph. D. , Joachim Wistuba, Ph

Cryopreservation of testicular tissue before long-term testicular cell culture does not alter in vitro cell dynamics Yoni Baert, M.Sc., Aude Braye, M.Sc.,

Akanksha Mehta, M.D., Darius A. Paduch, M.D., Ph.D.

Mara Curaba, M. Sc. , Jonathan Poels, M. Sc

Evaluation of diagnostic testis biopsy and the repetition of testicular sperm extraction surgeries in infertility patients Alayman Hussein, M.B.B.C.H.,

Human papillomavirus found in sperm head of young adult males affects the progressive motility Carlo Foresta, M.D., Andrea Garolla, M.D., Daniela Zuccarello,

Xenotransplantation of cryopreserved ovarian tissue from patients with ovarian tumors into SCID mice—no evidence of malignant cell contamination Laura.

Selective insulin-like growth factor-I antagonist inhibits mouse embryo development in a dose-dependent manner Jose Inzunza, Ph.D., Olle Danielsson,

Roberto Ensenat-Waser, Ph. D. , Antonio Pellicer, M. D. , Ph. D

Melatonin protects human spermatozoa from apoptosis via melatonin receptor– and extracellular signal–regulated kinase-mediated pathways Javier Espino,

Effects of hyperglycemia on the differential expression of insulin and insulin-like growth factor-I receptors in human normal peritoneal and adhesion.

Identification of ten novel genes involved in human spermatogenesis by microarray analysis of testicular tissue Ying-Hung Lin, M.Sc., Yung-Ming Lin,

Long-term survival of human spermatogonial stem cells in mouse testes

Adult somatic cells to the rescue: nuclear reprogramming and the dispensability of gonadal germ cells Charles A. Easley, Ph.D., David R. Latov, Calvin.

Stimulation of mitochondrial embryo metabolism by dichloroacetic acid in an aged mouse model improves embryo development and viability Nicole O. McPherson,

Upregulation of mRNA expression of vascular endothelial growth factor and its receptors by exogenous human chorionic gonadotropin in cultured oviduct.

Direct injection of vascular endothelial growth factor into the ovary of mice promotes follicular development Ramiro Quintana, M.D., Laura Kopcow, M.D.,

Integration of immunodeficiency virus in oocytes via intracytoplasmic injection: possible but extremely unlikely Marjan M.C. Steenvoorden, Ph.D., Marion.

Behavioral, cognitive, and motor performance and physical development of five-year-old children who were born after intracytoplasmic sperm injection with.

Tina Khanam, B.Sc., Geoffrey Burnstock, Ph.D., D.Sc.

Presentation transcript:

Enrichment of spermatogonial stem cells from long-term cultured human testicular cells Bita Nickkholgh, M.D., Sefika Canan Mizrak, V.M.D., Ph.D., Cindy M. Korver, B.Sc., Saskia K.M. van Daalen, B.Sc., Andreas Meissner, M.D., Sjoerd Repping, Ph.D., Ans M.M. van Pelt, Ph.D. Fertility and Sterility Volume 102, Issue 2, Pages 558-565.e5 (August 2014) DOI: 10.1016/j.fertnstert.2014.04.022 Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Enrichment of spermatogonia in sorted cells. (A) Fold change expression of ITGA6 and GPR125 in various sorted fractions compared with nonsorted cultured testicular cells derived from two patients (two independent cultures for each patient; mean ± SEM). *P≤.05. (B) Relative enrichment of spermatogonia as measured by means of fold change expression (qPCR) in sorted populations compared with nonsorted populations of cultured testicular cells (mean ± SEM) of ZBTB16 (n = 4 independent cultures with sortings for each patient), UCHL1 (n = 4 independent cultures with sortings for each patient) and ID4 (n = 2 independent cultures with sortings for each patient). Fertility and Sterility 2014 102, 558-565.e5DOI: (10.1016/j.fertnstert.2014.04.022) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Figure 2 COT-1 positive single, paired, and multiple cell human SSC colonies (red, first column) after xenotransplantation of nonsorted cells to immunodeficient mouse testis; DAPI nuclear counterstaining (blue, second column); merged images of colocalization of COT-1 and DAPI (third column), and magnified view of colonies (fourth column). First row shows two COT-1+ colonies: one containing a single cell, and the other one containing multiple cells (four cells); second row shows two COT-1+ colonies containing pairs of cells. The scale bars in the large images and all magnified pictures are 10 μm. Fertility and Sterility 2014 102, 558-565.e5DOI: (10.1016/j.fertnstert.2014.04.022) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Number of human COT+ SSC colonies in the recipient mouse testes 8 weeks after transplantation of nonsorted, ITGA6 sorted (ITGA6+), HLA and ITGA6 sorted (HLA−/ITGA6+), GPR125 sorted (GPR125+), and HLA and GPR125 sorted (HLA−/GPR125+) cells. Numbers are expressed as the number of colonies per testis per 105 injected cells of the corresponding fraction. (A) Total number of human SSC colonies found in transplanted mice testis. (B) Number of SSC colonies containing single cells (green), pairs (blue), or multiple cells (purple). Fertility and Sterility 2014 102, 558-565.e5DOI: (10.1016/j.fertnstert.2014.04.022) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 1 Histology of testis of both patients (A) URO0034 and (B) URO0077. Both men show normal spermatogenesis. Fertility and Sterility 2014 102, 558-565.e5DOI: (10.1016/j.fertnstert.2014.04.022) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 2 Expression of ID4 in human testis and two independent long-term cultured testicular cells of both included patients. ID4 product size is 144 base pairs (bp). 50 bp ladder was used as an indicator. POLR2A used as reference. Fertility and Sterility 2014 102, 558-565.e5DOI: (10.1016/j.fertnstert.2014.04.022) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 3 ID4 Immunofluorescent staining showing ID4 protein expression (green) of this spermatogonial marker as shown in fine speckles pattern in the nucleus of cells in cytospins of the nonsorted and sorted fractions similar to the transplanted fractions. Columns from left to right: differential interference contrast (DIC) image, ID4 immunofluorencent staining, DAPI, merged pictures. Scale bar: 10 μm. Fertility and Sterility 2014 102, 558-565.e5DOI: (10.1016/j.fertnstert.2014.04.022) Copyright © 2014 American Society for Reproductive Medicine Terms and Conditions