Kazuhiro Takeuchi, Shigeru Okada, Shigefumi Yukihiro, Hajime Inoue

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The inhibitory effects of aluminum and iron on bone formation, in vivo and in vitro study Kazuhiro Takeuchi, Shigeru Okada, Shigefumi Yukihiro, Hajime Inoue Pathophysiology Volume 4, Issue 2, Pages 97-104 (June 1997) DOI: 10.1016/S0928-4680(97)00164-8

Fig. 1 Undecalcified bone sections with Villanueva bone stain. (A): Control (×100). (B): Al-NTA (×100). Severe increase of osteoid (★), indicating severe osteomalacia. (C): Fe-NTA (×100). Mild increase of osteoid (★), indicating osteomalacia. (D): Control (×100). Double tetracycline labeling (Arrow head). (E): Al-NTA (×100) No double labelings, suggesting almost complete cessation of bone mineralization. (F): Fe-NTA (×100). Decreased band of double labelings. Pathophysiology 1997 4, 97-104DOI: (10.1016/S0928-4680(97)00164-8)

Fig. 1 Undecalcified bone sections with Villanueva bone stain. (A): Control (×100). (B): Al-NTA (×100). Severe increase of osteoid (★), indicating severe osteomalacia. (C): Fe-NTA (×100). Mild increase of osteoid (★), indicating osteomalacia. (D): Control (×100). Double tetracycline labeling (Arrow head). (E): Al-NTA (×100) No double labelings, suggesting almost complete cessation of bone mineralization. (F): Fe-NTA (×100). Decreased band of double labelings. Pathophysiology 1997 4, 97-104DOI: (10.1016/S0928-4680(97)00164-8)

Fig. 1 Undecalcified bone sections with Villanueva bone stain. (A): Control (×100). (B): Al-NTA (×100). Severe increase of osteoid (★), indicating severe osteomalacia. (C): Fe-NTA (×100). Mild increase of osteoid (★), indicating osteomalacia. (D): Control (×100). Double tetracycline labeling (Arrow head). (E): Al-NTA (×100) No double labelings, suggesting almost complete cessation of bone mineralization. (F): Fe-NTA (×100). Decreased band of double labelings. Pathophysiology 1997 4, 97-104DOI: (10.1016/S0928-4680(97)00164-8)

Fig. 1 Undecalcified bone sections with Villanueva bone stain. (A): Control (×100). (B): Al-NTA (×100). Severe increase of osteoid (★), indicating severe osteomalacia. (C): Fe-NTA (×100). Mild increase of osteoid (★), indicating osteomalacia. (D): Control (×100). Double tetracycline labeling (Arrow head). (E): Al-NTA (×100) No double labelings, suggesting almost complete cessation of bone mineralization. (F): Fe-NTA (×100). Decreased band of double labelings. Pathophysiology 1997 4, 97-104DOI: (10.1016/S0928-4680(97)00164-8)

Fig. 1 Undecalcified bone sections with Villanueva bone stain. (A): Control (×100). (B): Al-NTA (×100). Severe increase of osteoid (★), indicating severe osteomalacia. (C): Fe-NTA (×100). Mild increase of osteoid (★), indicating osteomalacia. (D): Control (×100). Double tetracycline labeling (Arrow head). (E): Al-NTA (×100) No double labelings, suggesting almost complete cessation of bone mineralization. (F): Fe-NTA (×100). Decreased band of double labelings. Pathophysiology 1997 4, 97-104DOI: (10.1016/S0928-4680(97)00164-8)

Fig. 1 Undecalcified bone sections with Villanueva bone stain. (A): Control (×100). (B): Al-NTA (×100). Severe increase of osteoid (★), indicating severe osteomalacia. (C): Fe-NTA (×100). Mild increase of osteoid (★), indicating osteomalacia. (D): Control (×100). Double tetracycline labeling (Arrow head). (E): Al-NTA (×100) No double labelings, suggesting almost complete cessation of bone mineralization. (F): Fe-NTA (×100). Decreased band of double labelings. Pathophysiology 1997 4, 97-104DOI: (10.1016/S0928-4680(97)00164-8)

Fig. 2 Undecalcified bone sections with histochemical stain. (A): Al-NTA (×200). Al deposition at the interface of osteoid and mineralized bone and along the cement line, noted as red lines. (B): Fe-NTA (×200). Diffuse Fe deposition in the cytoplasm of osteoblasts. Pathophysiology 1997 4, 97-104DOI: (10.1016/S0928-4680(97)00164-8)

Fig. 2 Undecalcified bone sections with histochemical stain. (A): Al-NTA (×200). Al deposition at the interface of osteoid and mineralized bone and along the cement line, noted as red lines. (B): Fe-NTA (×200). Diffuse Fe deposition in the cytoplasm of osteoblasts. Pathophysiology 1997 4, 97-104DOI: (10.1016/S0928-4680(97)00164-8)

Fig. 3 The growth curve of HuO9. (A): Al-NTA and (B): Fe-NTA. HuO9 cell failed to grow at 10−3 M. Pathophysiology 1997 4, 97-104DOI: (10.1016/S0928-4680(97)00164-8)

Fig. 4 The ALP activity. (A): Al-NTA and (B): Fe-NTA. ALP activity are expressed as the liberated p-nitrophenol. The activity is impaired by Al and Fe. *P<0.05, **P<0.01. Pathophysiology 1997 4, 97-104DOI: (10.1016/S0928-4680(97)00164-8)

Fig. 5 The collagen synthesis. (A): Al-NTA and (B): Fe-NTA. The slight decrease at 10−4 M Al, but not significantly. There was no significant difference ranging from 10−4 to 10−6 M. Pathophysiology 1997 4, 97-104DOI: (10.1016/S0928-4680(97)00164-8)

Fig. 5 The collagen synthesis. (A): Al-NTA and (B): Fe-NTA. The slight decrease at 10−4 M Al, but not significantly. There was no significant difference ranging from 10−4 to 10−6 M. Pathophysiology 1997 4, 97-104DOI: (10.1016/S0928-4680(97)00164-8)