Validation study of endogenous reference genes for normalization of quantitative real time PCR data in post mortem skin tissue  I. Gomes, K. Becker, M.A.

Slides:



Advertisements
Similar presentations
The most efficient STR loci in forensic genetics in population of central Poland R. Jacewicz, M. Jedrzejczyk, J. Berent Forensic Science International:
Advertisements

A comparison of AmpFlSTR Identifiler™ Kit versus AmpFlSTR Identifiler Plus™ Kit in challenging bone samples by using normal and increased PCR cycle number 
A comparison of AmpFlSTR Identifiler™ Kit versus AmpFlSTR Identifiler Plus™ Kit in challenging bone samples by using normal and increased PCR cycle number 
Kornkiat Vongpaisarnsin, Kittikamol Vongpaisarnsin 
A. Verzeletti, V. Cortellini, S. Cisana, S. Pretto, N. Cerri, F
V. Kallupurackal, T. Kausel, A. Sulzer, P. Voegeli, A. Kratzer 
Micro RNA profiling for the detection and differentiation of body fluids in forensic stain analysis  Peng Bai, Wei Deng, Li Wang, Bing Long, Kuanlin Liu,
R. Alghafri, W. Goodwin, S. Hadi 
N. Cerri, S. Cisana, V. Cortellini, A. Verzeletti, A. Agostino, F
An evaluation of miniSTR markers for casework applications
Stefania Turrina, Stefano Caratti, Domenico De Leo 
Performance of the RapidHIT™200
A. Barbaro, P. Cormaci, G. Falcone 
DTT quenches the passive reference signal in real-time PCR
Use of universal reporter primers in multiplex PCR of autosomal loci
A 1-year time course study of human RNA degradation in body fluids under dry and humid environmental conditions  M. Sirker, I. Gomes, M.A. Rothschild,
Forensic identification of 12 mammals species based on size variation of mitochondrial cytochrome b gene using multiplex PCR assay  M. Črček, K. Drobnič 
Wei Deng, Meili Lv, Li Wang, Peng Bai, Weibo Liang, Lin Zhang 
Probabilistic modelling for DNA mixture analysis
C. Davies, J. Thomson, F. Kennedy 
DNA typing of trace DNA recovered from different areas of sandals found at a homicide crime scene investigation: A comparative study  Samuel T.G. Ferreira,
Juliana G. Aquino, Juliana Jannuzzi, Elizeu F. Carvalho, Leonor Gusmão 
Establishment of Italian national DNA database and the central laboratory: Some aspects  R. Biondo, F. De Stefano  Forensic Science International: Genetics.
U. Ricci, I. Carboni, S. Iozzi, A. Gozzini, F. Torricelli 
J.P. Pamplona, F. Freitas, L. Pereira 
Quantification of trace amounts of human and non-human mitochondrial DNA (mtDNA) using SYBR Green and real time PCR  Shanan S. Tobe, Adrian Linacre  Forensic.
A custom software solution for forensic mtDNA analysis of MiSeq data
R. Alghafri, S. Alhammadi, K. Amiri 
Population data for SE33 locus in United Arab Emirates Arab population
Development of a SNP-STRs multiplex for forensic identification
5 miRNA expression analyze in post-mortem interval (PMI) within 48h
D. Zaumsegel, R. Pereira, M. A. Rothschild, C. Phillips, L. Gusmão, P
S. Iyavoo, A. Wolejko, D. Furmanczyk, R. Graham, R. Myers, T. Haizel 
Andréa M. Oliveira, Leonor Gusmão, Peter M. Schneider, Iva Gomes 
Oskar Hansson, Peter Gill 
Body fluid mixtures: Resolution using forensic microRNA analysis
High resolution melting (HRM) of forensically informative SNPs
Evaluating DNA profiles using peak heights, allowing for dropin, dropout and stutters  Roberto Puch-Solis  Forensic Science International: Genetics Supplement.
Quadruplex real-time PCR for forensic DNA quantitation
New RNA methods for the identification of body fluids and cell types
A comparison of AmpFlSTR Identifiler™ Kit versus AmpFlSTR Identifiler Plus™ Kit in challenging bone samples by using normal and increased PCR cycle number 
Distribution of Y chromosome haplogroup Q in Greenlanders
Are these food products fraudulent
Analysis of forensic samples in Banco Nacional de Datos Genéticos
Introducing a latent variable approach for finding populations in a forensic DNA database  Maarten Kruijver  Forensic Science International: Genetics.
Determination of siblings: A special case report from Halle
On the exact distribution of the numbers of alleles in DNA mixtures
Low-cost direct PCR for aged and processed wildlife sample analysis
N. Scaramozzino, P. Terrenoire, L. Baron 
Testing the behavior of GlobalFiler® PCR amplification kit with degraded and/or inhibited biological samples  V. Bogas, M. Carvalho, F. Corte-Real, M.J.
M. Hara, T. Masuda, A. Takada, T. Miyazaki, H. Suzuki, A. Kido, K
Semen detection: A retrospective overview from 2010
Application of less primer method to commercial kits
Ancestry proportions in urban populations of Argentina
DNA persistance in soft tissues exposed to extreme environments
Kpop: A Python package for population genetics analysis
Zhilong Li, Peng Bai, Duo Peng, Bing Long, Lin Zhang, Weibo Liang 
Simulated approach to estimate the number and combination of known/unknown contributors in mixed DNA samples using 15 short tandem repeat loci  Sho Manabe,
Molecular “eyewitness”: Forensic prediction of phenotype and ancestry
Monique Wesselink, Irene Kuiper 
Effects of the most common methods for the enhancement of latent fingerprints on DNA extraction from forensic samples  S. Gino, M. Omedei  Forensic Science.
M. Lurdes Pontes, M. Fátima Pinheiro 
Li Wang, P.M. Schneider, M.A. Rothschild, P. Bai, W. Liang, Lin Zhang 
Development and validation of a human DNA quantification and sex determination approach based on real time PCR followed by high resolution melting analysis 
Genetic data for the locus SE33 in a Southern Italy population with AmpFlSTR NGM SElect™ PCR amplification kit  A. Barbaro, P. Cormaci, A. La Marca  Forensic.
Development of a 24-plex Y chromosomal STR loci typing system
Statistical aspects of familial searching
Steffen L. Lauritzen, Anjali Mazumder 
Discrepancies between forensic DNA databases
A.M. Ölçen, G. Filoğlu, H. Altunçul, S. Erdem, Ö. Bülbül 
Presentation transcript:

Validation study of endogenous reference genes for normalization of quantitative real time PCR data in post mortem skin tissue  I. Gomes, K. Becker, M.A. Rothschild, P.M. Schneider  Forensic Science International: Genetics Supplement Series  Volume 4, Issue 1, Pages e156-e157 (January 2013) DOI: 10.1016/j.fsigss.2013.10.081 Copyright © 2013 Elsevier Ireland Ltd Terms and Conditions

Fig. 1 geNorm expression stability plots. The average expression stability M of 10 candidate reference control genes in skin wounds (A) and in normal skin reference samples (B). Genes with the lowest M values have the most stable expression. Forensic Science International: Genetics Supplement Series 2013 4, e156-e157DOI: (10.1016/j.fsigss.2013.10.081) Copyright © 2013 Elsevier Ireland Ltd Terms and Conditions