Genetics of E. coli fimbriae.

Slides:

Advertisements

Similar presentations

Mechanisms of Bcl-2 in Programmed Cell Death Laura Beth Hill St. Edward’s University.

Advertisements

PROTEIN TRAFFICKING AND LOCALIZATION PROTEINS SYNTHESIZED IN CYTOPLASM, BUT BECOME LOCALIZED IN CYTOPLASM CYTOPLASMIC MEMBRANE PERIPLASM OUTER MEMBRANE.

Lecture 1: Metabolism and Assembly Reactions Reading assignments in Text: Lengeler et al Text:pages Metabolic overview Text:pages ,

Gene Expression and Regulation

Type III Secretion System

Studies of protein trap expression We compared the apparent intensity of GFP-protein staining in the GSCs with the RNA level of the corresponding gene.

Additional file 8: Estimation of biological variations

10-3 Protein Synthesis Together, all 3 types of RNA synthesize proteins. Proteins are polymers. Polypeptides linked by peptide bonds. Made of 20 different.

The same gene can have many versions.

There are four levels of structure in proteins

Transfer of information from DNA

The same gene can have many versions.

The same gene can have many versions.

The same gene can have many versions.

The same gene can have many versions.

The same gene can have many versions.

Volume 113, Issue 5, Pages (May 2003)

The same gene can have many versions.

The same gene can have many versions.

Characteristic stacked-brick pattern observed when EAEC is cultured on HEp-2 cells. Characteristic stacked-brick pattern observed when EAEC is cultured.

Classic mechanisms of action of ETEC toxins (see the text for details and additional proposed mechanisms). Classic mechanisms of action of ETEC toxins.

NAD utilization in Pasteurellaceae.

The human norovirus genome.

(Left) Selected IgM immunoblot reactivities.

The same gene can have many versions.

Pathogenic schemes of diarrheagenic E. coli.

Phylogenetic tree constructed by the neighbor-joining method, showing the position of Peptostreptococcus species within Clostridium rRNA clusters XI, XIII,

The same gene can have many versions.

RND efflux operons in P. aeruginosa.

HDAF- and hCEACAM1-4L-associated lipid raft signaling pathways involved in Afa/Dr DAEC pathogenesis. hDAF- and hCEACAM1-4L-associated lipid raft signaling.

Genes involved in EPEC pathogenesis.

Characteristics of the new family of telomeric Cryptosporidium proteins, Cops-1 and Chos-1. Characteristics of the new family of telomeric Cryptosporidium.

MexT-associated downregulation of oprD expression.

The same gene can have many versions.

Coregulation of mexEF-oprN and oprD in P. aeruginosa.

Examples of PAI of various pathogens.

Bactericidal effect of probiotic L

Interaction of EAEC with the intestinal epithelium.

Data from in vitro competition experiments with E

Phylogenetic tree of intestinal pathogenic E. coli.

Fluconazole tissue and fluid concentrations in humans as multiples of the maximal or simultaneously measured concentration in plasma (μg/ml) after systemic.

Regulation of expression of the AcrAB-TolC efflux system of E. coli.

Location of drug efflux pumps and pathways of drug influx and efflux across the OM and IM in Gram-negative bacteria. Location of drug efflux pumps and.

Summary of the distribution and genetic context of the OXA-type enzymes in Acinetobacter baumannii. Summary of the distribution and genetic context of.

The same gene can have many versions.

Fabrizio Dutly, and Martin Altwegg Clin. Microbiol. Rev. 2001; doi:10

Staphylococcus aureus and Staphylococcus epidermidis cell surface proteins, known as microbial surface components recognizing adhesive matrix molecules.

T4P gene clusters in C. perfringens and C. difficile.

Mobile genetic elements associated with bla OXA genes.

Membrane-associated proteins expressed by human epithelial cells that function as receptors for Afa/Dr adhesins. Membrane-associated proteins expressed.

Western blot assay of an acute MIF-positive serum showing reactivity with the 135- and 115-kDa specific protein antigens of Rickettsia conorii and Rickettsia.

Cross talk between the virulence elements.

Sequence of helix 18 of the 16S rRNA genes (corresponding to Escherichia coli positions 430 to 500) of different Mycobacterium species. Sequence of helix.

Proposed mechanisms of Ag presentation.

Structure and function of RND efflux pumps in P. aeruginosa.

Receptor clustering by Afa/Dr DAEC

Molecular phylogenetic analysis of RNA polymerase II largest-subunit protein sequences from various trichomonads, including D. fragilis. Molecular phylogenetic.

Phylogenetic trees for fusion (F) and attachment (G) genes of selected HMPV isolates. Phylogenetic trees for fusion (F) and attachment (G) genes of selected.

Pathogenesis of oncogenic HPV

Adherence patterns of enteropathogenic E

Domain structure of TLR2 (A) and Nod2 (B).

Phylogenetic tree of Shigella, EIEC, and nonpathogenic E

Adherence patterns of enteric E. coli.

Different signaling pathways of TLR2, Nod, and TNFR1 in response to ligand, resulting in activation of NF-κB, the nuclear transcriptional factor responsible.

Overview of the regulatory networks in expression of group A streptococcal virulence genes. +, positive regulation; —, negative regulation. Overview of.

The Alp family of proteins.

Comparison of the van gene clusters.

Schematic representation of the relationships between acid resistance (urease activity and urea transport), nitrogen metabolism (ammonia production), metal.

Cartoon illustrating the genomic organization of a typical mucosal high-risk HPV. The genome contains early and late regions, which relate to the positions.

Diagrammatic view of the locations of genes encoding classical HLA class I (HLA-A, -B, -C, -E, -F, and -G) and class II (DR, DQ, DP, and DM) molecules.

Presentation transcript:

Genetics of E. coli fimbriae. Genetics of E. coli fimbriae. Genes required for the expression of functional pili are characteristically linked in gene clusters. The genetic organization of these clusters is illustrated for ETEC fimbriae CS1, CFA/I, CS3, and CS6, and for members of the Dr family, found in DAEC and EAEC. Italicized terms in parentheses represent the gene designations, to be followed by the specific letter under the corresponding arrow to the right. Arrows of similar fill pattern have genetic and functional homology; black arrows represent structural subunits. The known functions of the genes in the Dr cluster are listed below the corresponding genes. These functions can be extrapolated to arrows of similar fill pattern in the CS3 and CS6 gene cluster. The usher and chaperone genes from the Dr, CS6, and CS3 clusters have homology to the genes serving these functions inpap fimbriae: usher proteins are OMPs which serve as pores for the transport and assembly of the fimbrial shaft; fimbrial chaperones bind to the fimbrial subunit proteins in the periplasmic space and prevent premature folding and degradation. CS1 and CFA/I accessory genes, required for assembly and transport of the fimbriae, are homologous to each other but not to CS3, CS6, or the Dr family. CS6 has an unusual organization in that the first two genes of the cluster apparently encode heterologous major subunit proteins (699); the significance of this feature is not yet understood. James P. Nataro, and James B. Kaper Clin. Microbiol. Rev. 1998; doi:10.1128/CMR.11.1.142