Prevalence of Shiga toxin-producing Shigella species isolated from French travellers returning from the Caribbean: an emerging pathogen with international.

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Prevalence of Shiga toxin-producing Shigella species isolated from French travellers returning from the Caribbean: an emerging pathogen with international implications M.D. Gray, D.W. Lacher, S.R. Leonard, J. Abbott, S. Zhao, K.A. Lampel, E. Prothery, M. Gouali, F.-X. Weill, A.T. Maurelli Clinical Microbiology and Infection Volume 21, Issue 8, Pages 765.e9-765.e14 (August 2015) DOI: 10.1016/j.cmi.2015.05.006 Copyright © 2015 Terms and Conditions

Fig. 1 stx-encoding Shigella species from French travellers release a functional toxin. Overnight supernatants were serially diluted ten-fold in medium, and applied to Vero cells to test for toxicity. Stx from Shigella dysenteriae 1 was included as a positive control. CD50/mL is defined as the reciprocal of the dilution of Stx that kills 50% of Vero cells. Data represent an average of three independent experiments. Clinical Microbiology and Infection 2015 21, 765.e9-765.e14DOI: (10.1016/j.cmi.2015.05.006) Copyright © 2015 Terms and Conditions

Fig. 2 stx-encoding prophage sequence similarity. (a) Single-nucleotide polymorphisms (SNPs), insertion/deletion (indel)sites and IS elements within the prophage sequences of the isolates investigated. SNP locations are indicated by vertical black lines, and indel sites by blue lines. The locations and orientations of the IS1 and IS2 elements are indicated by the green and red arrows, respectively. (b) SNP-based phylogenetic relationships of the prophages. Clinical Microbiology and Infection 2015 21, 765.e9-765.e14DOI: (10.1016/j.cmi.2015.05.006) Copyright © 2015 Terms and Conditions

Fig. 3 Dendrogram of pulsed-field gel electrophoresis (PFGE) analysis (XbaI) of Stx-producing Shigella. The PFGE patterns from seven of the Stx-producing Shigella isolates from French travellers (BS1044, BS1046, BS1042, BS1043, BS1045, BS1041, and BS1047) are compared with that of BS937 (PulseNet PFGE pattern JZXX01.0357). Clinical Microbiology and Infection 2015 21, 765.e9-765.e14DOI: (10.1016/j.cmi.2015.05.006) Copyright © 2015 Terms and Conditions