Immunolocalization and temporal distribution of cytokine expression during the development of vein graft intimal hyperplasia in an experimental model

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Presentation transcript:

Immunolocalization and temporal distribution of cytokine expression during the development of vein graft intimal hyperplasia in an experimental model Peter L. Faries, MD, Michael L. Marin, MD, Frank J. Veith, MD, Jose A. Ramirez, MD, William D. Suggs, MD, Richard E. Parsons, MD, Luis A. Sanchez, MD, Ross T. Lyon, MD Journal of Vascular Surgery Volume 24, Issue 3, Pages 463-471 (September 1996) DOI: 10.1016/S0741-5214(96)70203-3 Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 1 Schematic representation of vein graft model preparation and microsurgical technique. Inguinal fat pad is exposed and superficial epigastric vein is dissected free from surrounding tissue. Femoral vessels are isolated in preparation for grafting. Procedure is performed bilaterally in each rat. Inset, Superficial epigastric vein is interposition grafted to femoral artery in end-to-end fashion. Journal of Vascular Surgery 1996 24, 463-471DOI: (10.1016/S0741-5214(96)70203-3) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 Cytokine expression quantified as percentage of immunopositive cells per high-power field in (A) neointima/media and (B) endothelium. Journal of Vascular Surgery 1996 24, 463-471DOI: (10.1016/S0741-5214(96)70203-3) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 Cytokine expression quantified as percentage of immunopositive cells per high-power field in (A) neointima/media and (B) endothelium. Journal of Vascular Surgery 1996 24, 463-471DOI: (10.1016/S0741-5214(96)70203-3) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 3 Immunohistochemical analysis of PDGF-AA protein in intimal hyperplasia 2 weeks after grafting procedure. A, Diffuse immunostaining throughout neointima/media is seen (100×). B, At higher magnification, predominant cytoplasmic staining pattern (arrow) is evedent (400×). C, Absorption control (HRP/DAB), hematoxylin). Journal of Vascular Surgery 1996 24, 463-471DOI: (10.1016/S0741-5214(96)70203-3) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 3 Immunohistochemical analysis of PDGF-AA protein in intimal hyperplasia 2 weeks after grafting procedure. A, Diffuse immunostaining throughout neointima/media is seen (100×). B, At higher magnification, predominant cytoplasmic staining pattern (arrow) is evedent (400×). C, Absorption control (HRP/DAB), hematoxylin). Journal of Vascular Surgery 1996 24, 463-471DOI: (10.1016/S0741-5214(96)70203-3) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 3 Immunohistochemical analysis of PDGF-AA protein in intimal hyperplasia 2 weeks after grafting procedure. A, Diffuse immunostaining throughout neointima/media is seen (100×). B, At higher magnification, predominant cytoplasmic staining pattern (arrow) is evedent (400×). C, Absorption control (HRP/DAB), hematoxylin). Journal of Vascular Surgery 1996 24, 463-471DOI: (10.1016/S0741-5214(96)70203-3) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 4 Immunohistochemical analysis of IL-1β protein in intimal hyperplasia at 6 hours. (A, IL-1β; B, absorption control; HRP/DAB 400×). Arrow indicates typical granular staining pattern of macrophage IL-1β expression. Journal of Vascular Surgery 1996 24, 463-471DOI: (10.1016/S0741-5214(96)70203-3) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 4 Immunohistochemical analysis of IL-1β protein in intimal hyperplasia at 6 hours. (A, IL-1β; B, absorption control; HRP/DAB 400×). Arrow indicates typical granular staining pattern of macrophage IL-1β expression. Journal of Vascular Surgery 1996 24, 463-471DOI: (10.1016/S0741-5214(96)70203-3) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 5 Immunohistochemical analysis of bFGF protein in intimal hyperplasia 2 days after grafting procedure. A, Intense subendothelial distribution of bFGF is seen (arrows). Sporadic neointimal/medial staining is also present (100×). B, Absorption control (HRP/DAB, hematoxylin). Journal of Vascular Surgery 1996 24, 463-471DOI: (10.1016/S0741-5214(96)70203-3) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 5 Immunohistochemical analysis of bFGF protein in intimal hyperplasia 2 days after grafting procedure. A, Intense subendothelial distribution of bFGF is seen (arrows). Sporadic neointimal/medial staining is also present (100×). B, Absorption control (HRP/DAB, hematoxylin). Journal of Vascular Surgery 1996 24, 463-471DOI: (10.1016/S0741-5214(96)70203-3) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 6 Immunohistochemical analysis of INFγ protein in control vein. A, Control epigastric vein demonstrates significant neointimal/medial expression of INFγ protein (100×) (arrows). After grafting procedure, no INFγ expression is present. B, Absorption control (HRP/DAB, hematoxylin). Journal of Vascular Surgery 1996 24, 463-471DOI: (10.1016/S0741-5214(96)70203-3) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 6 Immunohistochemical analysis of INFγ protein in control vein. A, Control epigastric vein demonstrates significant neointimal/medial expression of INFγ protein (100×) (arrows). After grafting procedure, no INFγ expression is present. B, Absorption control (HRP/DAB, hematoxylin). Journal of Vascular Surgery 1996 24, 463-471DOI: (10.1016/S0741-5214(96)70203-3) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 7 Immunohistochemical analysis of α-SMC actin protein in intimal hyperplasia 1 week after grafting procedure. Immunostaining-identified smooth muscle cells are limited to neointima/media (100×) (FITC-anti-α-SMC actin). Journal of Vascular Surgery 1996 24, 463-471DOI: (10.1016/S0741-5214(96)70203-3) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions