Intraepithelial lymphocytes David P. Hoytema van Konijnenburg, Daniel Mucida  Current Biology  Volume 27, Issue 15, Pages R737-R739 (August 2017) DOI: 10.1016/j.cub.2017.05.073 Copyright © 2017 Elsevier Ltd Terms and Conditions

Figure 1 Intraepithelial localization of TCRγδ+ IELs in the intestine. Intravital microscopy (IVM, 2-photon) imaging of ileal villi in TCRγδGFP (GFP = green channel) mice. Mice were injected with Hoechst (blue channel) before imaging to visualize all nuclei (blue). A luminal-to-crypt vertical orientation is shown. Anatomical (sub)compartments of the intestinal mucosa are shown as: Lu, lumen; IE, intraepithelial compartment; LP, lamina propria compartment. The dashed line indicates a manually drawn border. (A) Complete time-lapse showing TCRγδ+ IEL distribution in anatomical compartments over time (30 minutes). (B) Zoomed-in, higher resolution (1 μm slices) image of an ex vivo fixed ileum villus region (about 10 μm thickness), showing the location of one TCRγδ+ IEL in relation to epithelial cells in the IE compartment and the LP. Images are representative of 20–22 villi from at least three independent experiments (courtesy of Bernardo Reis and David Hoytema van Konijnenburg, Mucida lab). For more information, see Sujino et al. (2016) Science 352, 1581–1586. Current Biology 2017 27, R737-R739DOI: (10.1016/j.cub.2017.05.073) Copyright © 2017 Elsevier Ltd Terms and Conditions