TCRγ-Chain Gene Rearrangement by PCR-Based GeneScan: Diagnostic Accuracy Improvement and Clonal Heterogeneity Analysis in Multiple Cutaneous T-Cell Lymphoma.

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TCRγ-Chain Gene Rearrangement by PCR-Based GeneScan: Diagnostic Accuracy Improvement and Clonal Heterogeneity Analysis in Multiple Cutaneous T-Cell Lymphoma Samples Renata Ponti, Maria T. Fierro, Pietro Quaglino, Bonello Lisa, Francia di Celle Paola, Ortoncelli Michela, Fava Paolo, Alessandra Comessatti, Mauro Novelli, Maria G. Bernengo Journal of Investigative Dermatology Volume 128, Issue 4, Pages 1030-1038 (April 2008) DOI: 10.1038/sj.jid.5701109 Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 SS patient no. 7. (a) Ambiguous GS pattern in skin biopsy at diagnosis; (b) biallelic TCRγ-GR (*) confirmed by comparison with blood pattern at diagnosis; (c) GS on sorted CD4+CD26-Vβ5.1+ circulating lymphocytes; (d) GS on sorted CD4+CD26-TCR-Vβ5.1- circulating lymphocytes. Journal of Investigative Dermatology 2008 128, 1030-1038DOI: (10.1038/sj.jid.5701109) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 SS patient no. 1. (a) Ambiguous GS pattern in skin biopsy at diagnosis; TCRVγII GR (*) confirmed by comparison with blood pattern; (b) GS pattern in blood sample at diagnosis showing a pathological GR (*) and a concomitant transient GR (**); (c) TCRVγII clonal GR in the second skin sample. Journal of Investigative Dermatology 2008 128, 1030-1038DOI: (10.1038/sj.jid.5701109) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 CD3 expression and corresponding GS analysis in MF patient no. 9. (a, d) The first non-diagnostic biopsy showing an oligoclonal pattern; (b, e) second biopsy with borderline histopathology showing the stable clonal TCRγ-GR 296VγIII/IV Jγ (*) and a dominant transient VγIJγ GR (**); (c, f) third biopsy with a massive infiltrate confirming the clonal VγIII/IV Jγ GR (sequence: Vγ10Jγ2); (d–f) CD3 staining showing a progressive increase in dermal and epidermal lymphoid infiltrate in the three consecutive samples. Journal of Investigative Dermatology 2008 128, 1030-1038DOI: (10.1038/sj.jid.5701109) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions