Quantifying Instrumental Artifacts in Folding Kinetics Measured by Single-Molecule Force Spectroscopy Krishna Neupane, Michael T. Woodside Biophysical.

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Quantifying Instrumental Artifacts in Folding Kinetics Measured by Single-Molecule Force Spectroscopy Krishna Neupane, Michael T. Woodside Biophysical Journal Volume 111, Issue 2, Pages 283-286 (July 2016) DOI: 10.1016/j.bpj.2016.06.011 Copyright © 2016 Biophysical Society Terms and Conditions

Figure 1 Analysis of rates. (A) Extension trajectory for hairpin 30R50/T4 at constant force. (Upper inset) Schematic of measurement showing hairpin attached to beads via DNA handles. (Right inset) Probability density from trajectory. (B) Decay of extension autocorrelation in unfolded state yields τA. (C) PMF from the extension trajectory, yielding κAb, κAw, and ΔGA‡. (D) Landscape obtained by deconvolution of PMF. To see this figure in color, go online. Biophysical Journal 2016 111, 283-286DOI: (10.1016/j.bpj.2016.06.011) Copyright © 2016 Biophysical Society Terms and Conditions

Figure 2 Constant trap position measurements. (A) Extension trajectory for hairpin 20TS06/T4. (B) Decay of extension autocorrelation in unfolded state yields τA. (C) PMF from extension trajectory yields κAb, κAw, and ΔGA‡. To see this figure in color, go online. Biophysical Journal 2016 111, 283-286DOI: (10.1016/j.bpj.2016.06.011) Copyright © 2016 Biophysical Society Terms and Conditions