Comparison of capsular polysaccharide production by AB307-0294 (wt) and AB307.70 (ΔbfmR). Comparison of capsular polysaccharide production by AB307-0294.

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Comparison of capsular polysaccharide production by AB307-0294 (wt) and AB307.70 (ΔbfmR). Comparison of capsular polysaccharide production by AB307-0294 (wt) and AB307.70 (ΔbfmR). Capsule production was assessed by Western blotting. The monoclonal antibody 13D6, which has been shown to recognize the K1 capsule produced by AB307-0294, was used to detect capsule. (A) Cell-associated capsule (cell pellet) and capsule in culture supernatant (sup) was assessed after AB307-0294 and AB307.70 were grown in rich Luria-Bertani laboratory medium at 37°C and 21°C. (B) Cell-associated capsule was assessed after AB307-0294 and AB307.70 were grown in rich LB laboratory medium, minimal medium (MM), or human ascites fluid at 37°C. Thomas A. Russo et al. mSphere 2016; doi:10.1128/mSphere.00082-16