The role of heat shock protein 70 (Hsp 70) in male infertility: is it a line of defense against sperm DNA fragmentation? Gül Özdemirler Erata, Ph.D.,

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The role of heat shock protein 70 (Hsp 70) in male infertility: is it a line of defense against sperm DNA fragmentation? Gül Özdemirler Erata, Ph.D., Necla Koçak Toker, Ph.D., Özgür Durlanık, M.D., Ateş Kadıoğlu, M.D., Gülşen Aktan, BCHM M.S., Gülçin Aykaç Toker, Ph.D. Fertility and Sterility Volume 90, Issue 2, Pages 322-327 (August 2008) DOI: 10.1016/j.fertnstert.2007.06.021 Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Expression profiles of Hsp 70 protein of spermatozoa from fertile and infertile men grouped as asthenospermics and oligoasthenospermics. A protein sample from fertile group was applied onto lane 1 in each assay performed and its value was taken as 100% arbitrarily to compare with other samples (A). The blots were stripped and subsequently reprobed with an α-tubulin antibody for normalization of loading (B). The Western blot picture of α-tubulin is representative blots from random patients in the study. Fertility and Sterility 2008 90, 322-327DOI: (10.1016/j.fertnstert.2007.06.021) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Inverse correlation between TUNEL-positive spermatozoa and sperm concentration. Fertility and Sterility 2008 90, 322-327DOI: (10.1016/j.fertnstert.2007.06.021) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Inverse correlation between TUNEL-positive cells and motility of spermatozoa. Fertility and Sterility 2008 90, 322-327DOI: (10.1016/j.fertnstert.2007.06.021) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 4 Positive correlation between Hsp70 expression and TUNEL-positive cells. Fertility and Sterility 2008 90, 322-327DOI: (10.1016/j.fertnstert.2007.06.021) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions