Volume 16, Issue 1, Pages 31-37 (June 2009) Studies on preventive effects of diphenyl diselenide on acetaminophen-induced hepatotoxicity in rats  Ethel A. Wilhelm, Cristiano R. Jesse, Marlon R. Leite, Cristina W. Nogueira  Pathophysiology  Volume 16, Issue 1, Pages 31-37 (June 2009) DOI: 10.1016/j.pathophys.2008.12.002 Copyright © 2008 Elsevier Ireland Ltd Terms and Conditions

Fig. 1 Photomicrography of the hepatic lobe segment (A) of a control animal, note the presence of sinusoid capillaries and hepatocyte strings around the centrilobular vein (CV) showing normal aspect; (B) of an AA-exposed animal, note intense cellular necrosis, characterized by the presence of Kupffer cells and other inflammatory cells mainly around of the CV, observe the hepatocytes with eosenophilia cytoplasmatic; (C) of a (PhSe)2-treated animal, note the cellular architecture with normal aspect; (D) of an AA+(PhSe)2-treated animal, note hepatic tissue around the CV with normal appearance, but vacuolated hepatocytes were noted in lateral areas. Strings of hepatocytes (headarrows), Kupffer cells (#); eosenophilia cytoplasmatic (e); necrosis (*); vacuolization (v), HE 100×. Pathophysiology 2009 16, 31-37DOI: (10.1016/j.pathophys.2008.12.002) Copyright © 2008 Elsevier Ireland Ltd Terms and Conditions

Fig. 2 Effect of pretreatment with (PhSe)2 on hepatic TBARS levels of rats exposed to AA. Data are reported as mean±S.D. of eight animals per group. (*) Denotes p<0.05 as compared to the control group (two-way ANOVA/Duncan). (#) Denotes p<0.05 as compared to the AA group (two-way ANOVA/Duncan). Pathophysiology 2009 16, 31-37DOI: (10.1016/j.pathophys.2008.12.002) Copyright © 2008 Elsevier Ireland Ltd Terms and Conditions

Fig. 3 Effect of pretreatment with (PhSe)2 on hepatic GST activity of rats exposed to AA. Data are reported as mean±S.D. of eight animals per group. (*) Denotes p<0.05 as compared to the control group (two-way ANOVA/Duncan). (#) Denotes p<0.05 as compared to the AA group (two-way ANOVA/Duncan). Pathophysiology 2009 16, 31-37DOI: (10.1016/j.pathophys.2008.12.002) Copyright © 2008 Elsevier Ireland Ltd Terms and Conditions

Fig. 4 Effect of pretreatment with (PhSe)2 on hepatic ascorbic acid levels of rats exposed to AA. Data are reported as mean±S.D. of eight animals per group. * Denotes p<0.05 as compared to the control group (two-way ANOVA/Duncan). (#) Denotes p<0.05 as compared to the AA group (two-way ANOVA/Duncan). Pathophysiology 2009 16, 31-37DOI: (10.1016/j.pathophys.2008.12.002) Copyright © 2008 Elsevier Ireland Ltd Terms and Conditions

Fig. 5 Effect of pretreatment with (PhSe)2 on hepatic δ-ALA-D activity of rats exposed to AA. Data are reported as mean±S.D. of eight animals per group. (*) Denotes p<0.05 as compared to the control group (two-way ANOVA/Duncan). (#) Denotes p<0.05 as compared to the AA group (two-way ANOVA/Duncan). Pathophysiology 2009 16, 31-37DOI: (10.1016/j.pathophys.2008.12.002) Copyright © 2008 Elsevier Ireland Ltd Terms and Conditions