Ovarian cryopreservation: experimental or established and a cure for the menopause?  W.H. Wallace, T.W. Kelsey, R.A. Anderson  Reproductive BioMedicine Online  Volume 25, Issue 2, Pages 93-95 (August 2012) DOI: 10.1016/j.rbmo.2012.06.008 Copyright © 2012 Reproductive Healthcare Ltd. Terms and Conditions

Supplementary Figure 1 Using our normative model of non-growing follicles (NGF) decline (Wallace and Kelsey, 2010) and assuming that after oophorectomy one third of the tissue is re-implanted, and during re-vascularisation two thirds of the NGF present are lost we can calculate the age at which fewer than 1000 NGF will remain after freezing, thawing and transplantation. If we accept that menopause occurs when there are 1000 or fewer NGF present then this figure demonstrates the percent of the healthy population that is not expected to benefit from ovarian tissue cryopreservation. So at age 36.9years we estimate that in 40% of the healthy population insufficient NGF will remain after cryopreservation to sustain ovarian function. Similarly at age 40years we estimate that 60% of the healthy population are unlikely to benefit from ovarian cryopreservation. Reproductive BioMedicine Online 2012 25, 93-95DOI: (10.1016/j.rbmo.2012.06.008) Copyright © 2012 Reproductive Healthcare Ltd. Terms and Conditions