Underestimation of house dust mite–specific IgE with extract-based ImmunoCAPs compared with molecular ImmunoCAPs Huey-Jy Huang, MSc, Yvonne Resch-Marat,

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Underestimation of house dust mite–specific IgE with extract-based ImmunoCAPs compared with molecular ImmunoCAPs Huey-Jy Huang, MSc, Yvonne Resch-Marat, PhD, Azahara Rodriguez-Dominguez, MSc, Kuan-Wei Chen, PhD, Renata Kiss, MSc, Petra Zieglmayer, MD, René Zieglmayer, DI, Patrick Lemell, PhD, Friedrich Horak, MD, Rudolf Valenta, MD, Susanne Vrtala, PhD Journal of Allergy and Clinical Immunology Volume 142, Issue 5, Pages 1656-1659.e9 (November 2018) DOI: 10.1016/j.jaci.2018.07.010 Copyright © 2018 The Authors Terms and Conditions

Fig 1 Comparison of allergen-specific IgE levels measured with commercial HDM ImmunoCAPs and molecular ImmunoCAPs for sera from patients with HDM allergy from cohort 1, cohort 2, and cohort 1 plus cohort 2. Significant differences of IgE levels are indicated as follows: *P < .05, ***P < .001, and ****P < .0001. Journal of Allergy and Clinical Immunology 2018 142, 1656-1659.e9DOI: (10.1016/j.jaci.2018.07.010) Copyright © 2018 The Authors Terms and Conditions

Fig E1 Qualitative determination of IgE reactivity to HDM allergen molecules determined by using RAST-based dot blotting. Dot-blotted HDM allergens and BSA were tested for IgE reactivity with sera from 27 patients with mite allergy (cohort 1, P1–P27; Table E1, left column) and serum from a nonallergic subject (NC). Journal of Allergy and Clinical Immunology 2018 142, 1656-1659.e9DOI: (10.1016/j.jaci.2018.07.010) Copyright © 2018 The Authors Terms and Conditions

Fig E2 Correlations of IgE levels to HDM allergen extract and HDM allergen molecules measured by using different assays. IgE levels to HDM allergen extract–based ImmunoCAPs (CAPextract) to a molecular ImmunoCAP containing Der p 1, 2, 5, 7, 21, and 23 (CAPallergen) or to ImmunoCAP ISAC chips (∑ISAC) were measured in sera from patients with HDM allergy (top, cohort 1; bottom, cohort 2) and correlated with each other. Correlation coefficients (r), regression equations, and statistical significances (P values) are shown for each correlation. Journal of Allergy and Clinical Immunology 2018 142, 1656-1659.e9DOI: (10.1016/j.jaci.2018.07.010) Copyright © 2018 The Authors Terms and Conditions

Fig E3 Correlations of IgE levels to HDM allergen extract and HDM allergen molecules measured by using different assays. IgE levels to HDM allergen extracts (CAP extract) or to a mix of allergen molecules (Der p 1, 2, 5, 7, 21, and 23) bound to ImmunoCAP (CAP allergen) or ImmunoCAP ISAC chips (∑ISAC) or measured by means of ELISA (∑ELISA) were determined in sera from patients with HDM allergy of cohort 1. Correlation coefficients (r) and statistical significances (P values) are shown for each correlation. Journal of Allergy and Clinical Immunology 2018 142, 1656-1659.e9DOI: (10.1016/j.jaci.2018.07.010) Copyright © 2018 The Authors Terms and Conditions

Fig E4 Percentage differences in allergen-specific IgE levels measured with the molecular ImmunoCAP compared with the allergen extract–based ImmunoCAP for patients with HDM-specific IgE levels of greater than 20 kUA/L and less than 20 kUA/L (A) or IgE reactivity to 1 to 3 HDM allergens or IgE reactivity to 4 to 6 of the allergens (B) and in patients with and without IgE reactivity to each of the 6 allergens: Der p 1 (C), Der p 2 (D), Der p 5 (E), Der p 7 (F), Der p 21 (G), and Der p 23 (H). *P < .05 and **P < .01. Journal of Allergy and Clinical Immunology 2018 142, 1656-1659.e9DOI: (10.1016/j.jaci.2018.07.010) Copyright © 2018 The Authors Terms and Conditions

Fig E4 Percentage differences in allergen-specific IgE levels measured with the molecular ImmunoCAP compared with the allergen extract–based ImmunoCAP for patients with HDM-specific IgE levels of greater than 20 kUA/L and less than 20 kUA/L (A) or IgE reactivity to 1 to 3 HDM allergens or IgE reactivity to 4 to 6 of the allergens (B) and in patients with and without IgE reactivity to each of the 6 allergens: Der p 1 (C), Der p 2 (D), Der p 5 (E), Der p 7 (F), Der p 21 (G), and Der p 23 (H). *P < .05 and **P < .01. Journal of Allergy and Clinical Immunology 2018 142, 1656-1659.e9DOI: (10.1016/j.jaci.2018.07.010) Copyright © 2018 The Authors Terms and Conditions