A Precise Group Size in Dictyostelium Is Generated by a Cell-Counting Factor Modulating Cell–Cell Adhesion Céline Roisin-Bouffay, Wonhee Jang, David.

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A Precise Group Size in Dictyostelium Is Generated by a Cell-Counting Factor Modulating Cell–Cell Adhesion Céline Roisin-Bouffay, Wonhee Jang, David R Caprette, Richard H Gomer Molecular Cell Volume 6, Issue 4, Pages 953-959 (October 2000) DOI: 10.1016/S1097-2765(05)00082-1

Figure 1 Streams of smlA, Wild-Type Ax4, and countin Cells (A–C) Cells were developed in filter pads and photographed after 7 hr of development. Bar in (C) is 0.5 mm. (D–F) Similar streams of cells were fixed, stained, and sectioned for electron microscopy. Bar in (F) is 2 μm. Molecular Cell 2000 6, 953-959DOI: (10.1016/S1097-2765(05)00082-1)

Figure 2 Cell–Cell Adhesion in Wild-Type Ax4, smlA, and countin Cells during Development At the indicated times of development on filters, cells were collected, and cell–cell adhesion was assayed following Desbarats et al. 1994. (A) Total adhesion; (B) EDTA-resistant adhesion. Insert in (A) shows the results of assaying for the percentage of WT and countin cells in aggregates at 2 min after disaggregation rather than 20 min after disaggregation for cells at 6, 7, and 8 hr of development. (C) Protein samples from cells developing as in (A) were separated by SDS-PAGE, and protein blots were stained with anti-gp24 antibodies (left panels) or anti-gp80 antibodies (right panels). Molecular Cell 2000 6, 953-959DOI: (10.1016/S1097-2765(05)00082-1)

Figure 3 Counting Factor Affects Adhesion and Adhesion Affects Group Size (A) The effect of development in the presence of CM's from Ax4 wild-type, smlA, countin cells, and different concentrations of purified CF on the adhesion of Ax4 cells. Adhesion was measured at 6 hr of development. (B) The effect of anti-gp24 antibodies on the development of Ax4 cells. Pictures show cells after 10 hr of development. Bar is 1 mm. Molecular Cell 2000 6, 953-959DOI: (10.1016/S1097-2765(05)00082-1)

Figure 4 Computer Simulations of Stream Breakup (A) A column or stream of cells where the initial adhesion is either high (left panel), moderate (middle panel), or low (right panel). (B) A section of a stream at different times in the simulation showing the progress of the stream breakup. (C) Having the adhesion slowly increase with time also causes stream breakup (left panel), but the group size is dependent on the initial stream thickness. The right panel shows the effect of a diffusible factor slightly repressing the adhesion that increases with time. Molecular Cell 2000 6, 953-959DOI: (10.1016/S1097-2765(05)00082-1)