M. J. Medina-Pascual, S. Valdezate, G. Carrasco, P. Villalón, N

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Increase in isolation of Burkholderia contaminans from Spanish patients with cystic fibrosis M.J. Medina-Pascual, S. Valdezate, G. Carrasco, P. Villalón, N. Garrido, J.A. Saéz-Nieto Clinical Microbiology and Infection Volume 21, Issue 2, Pages 150-156 (February 2015) DOI: 10.1016/j.cmi.2014.07.014 Copyright © 2014 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

FIG. 1 Infection/colonization of patients with cystic fibrosis (n = 10) by Burkholderia contaminans clones and other species of the Burkholderia cepacia complex. Clinical Microbiology and Infection 2015 21, 150-156DOI: (10.1016/j.cmi.2014.07.014) Copyright © 2014 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

FIG. 2 Minimum spanning tree of all Burkholderia contaminans sequence types (STs) held by the multilocus sequence typing (MLST) website (http://pubmlst.org/bcc/). The size of each circle is related to the number of isolates submitted to the MLST website; colours indicate geographical origin; the number on each line indicates the level of genetic relationship between STs (1 for single-locus variant and 3 for triple-locus variant; there is no double-locus variant). ST102, ST404, ST482 and ST771 were detected in our study. Clinical Microbiology and Infection 2015 21, 150-156DOI: (10.1016/j.cmi.2014.07.014) Copyright © 2014 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

FIG. 3 Neighbour-joining tree inferred from the seven multilocus sequence typing (MLST) concatenated alleles, showing the phylogenetic relationships among members of the Burkholderia cepacia complex (Bcc). The type strains T of Bcc species were used for comparison when their sequences were available in the MLST database. The analysis was performed by MEGA6 software and the Kimura two-parameter method. The length of the branches corresponds to the number of base substitutions per site. Clinical Microbiology and Infection 2015 21, 150-156DOI: (10.1016/j.cmi.2014.07.014) Copyright © 2014 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

FIG. 4 Genetic diversity of Burkholderia contaminans isolates, characterized by SpeI pulsed-field gel electrophoresis (clustering at 80% similarity). All isolates were recovered from Spanish patients with cystic fibrosis between 2008 and 2012. aCodes assigned according to the date of submission of Burkholderia spp. strains to our laboratory. bSequence type. Clinical Microbiology and Infection 2015 21, 150-156DOI: (10.1016/j.cmi.2014.07.014) Copyright © 2014 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions