Gating strategies for Fig 3.

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Volume 37, Issue 3, Pages (September 2012)

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Supplemental Tables/Figures

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Presentation transcript:

Gating strategies for Fig 3. Gating strategies for Fig 3. (A) Animals were infected with 1 × 106 pfu Fluc CHIKV by footpad injection and monitored daily until 6 dpi when the virus-infected joint footpad was harvested and analyzed by flow cytometry. The following markers were used for immune profiling: live–dead, lineage (Ter119, CD19, and NK1.1), CD45, CD4, CD3, CD44, CXCR3, CCR6, CCR4, Tbet, RoRγT, and GATA3 (Table S2). (B) Animals were infected with 1 × 106 pfu Fluc CHIKV by footpad injection and monitored daily until 6 dpi when the virus-infected joint footpad was harvested, stimulated for 4 h (3 last hours with Brefeldin A and monensin), and followed by flow cytometry analysis. The following markers were used for immune profiling: live–dead, lineage (Ter119, CD19, and NK1.1), CD45, CD4, CD3, CD44, IFNγ, IL-4, IL-17, TNFα, and IL-10. Guillaume Carissimo et al. LSA 2019;2:e201900298 © 2019 Carissimo et al.