Dopamine Receptor Activation By Honey Bee Queen Pheromone

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Dopamine Receptor Activation By Honey Bee Queen Pheromone Kyle T. Beggs, Alison R. Mercer  Current Biology  Volume 19, Issue 14, Pages 1206-1209 (July 2009) DOI: 10.1016/j.cub.2009.05.051 Copyright © 2009 Elsevier Ltd Terms and Conditions

Figure 1 Homovanillyl Alcohol Shows No Agonist Activity on the D1-like Dopamine Receptors AmDOP1 and AmDOP2 (A and B) HEK293 cells expressing either AmDOP1 receptors (A) or AmDOP2 receptors (B) show a dose-dependent increase in intracellular cAMP levels in response to dopamine, but not in response to homovanillyl alcohol (HVA). Data are means ± SEM of three independent experiments. (C and D) Intracellular cAMP levels in Sf21 cells expressing AmDOP1 receptors (C) or AmDOP2 receptors (D). Dopamine (10 μM) increases cAMP levels in AmDOP1- and AmDOP2-expressing cells, whereas HVA (10 μM) has no effect on cAMP levels in these cells, nor does it block the response to 10 μM dopamine. Values are means ± SEM obtained from three independent experiments. Overall statistical significance was determined by one-way ANOVA followed by Tukey-Kramer tests. Letters above each bar indicate the significance of differences between groups. Groups that share a letter are not significantly different. F3,8 = 67.89, p < 0.0001 for (C); F3.8 = 25.99, p = 0.0002 for (D). Current Biology 2009 19, 1206-1209DOI: (10.1016/j.cub.2009.05.051) Copyright © 2009 Elsevier Ltd Terms and Conditions

Figure 2 HVA Acts as an Agonist at the D2-like Dopamine Receptor AmDOP3 (A) HEK293 cells expressing AmDOP3 receptors show a dose-dependent decrease in intracellular cAMP in response to treatment with either dopamine or HVA. Data are means ± SEM of three independent experiments. Statistical significance was determined via Student's two-tailed t test. (B) Effects of HVA and dopamine on Sf21 cells expressing either AmDOP3 receptors (white bars) or β-galactosidase (β-Gal) protein (gray bars). Dopamine (10 μM) causes a significant decrease in intracellular cAMP levels in AmDOP3-expressing cells (p = 0.0004). Treatment with 10 μM HVA also decreases cAMP levels in these cells (p = 0.0034). Data are expressed as a percentage of forskolin-stimulated cAMP levels in controls. Data are means ± SEM of eight independent experiments. Asterisks denote significant differences between AmDOP3-expressing cells and β-Gal-expressing cells exposed to the same ligand, as determined via Student's two-tailed t test. Current Biology 2009 19, 1206-1209DOI: (10.1016/j.cub.2009.05.051) Copyright © 2009 Elsevier Ltd Terms and Conditions

Figure 3 HVA Has No Activity on the Octopamine Receptor AmOA1 HEK293 cells expressing AmOA1 receptors show a dose-dependent increase in intracellular cAMP in response to octopamine but do not respond to HVA. Data are means ± SEM of three independent experiments. Current Biology 2009 19, 1206-1209DOI: (10.1016/j.cub.2009.05.051) Copyright © 2009 Elsevier Ltd Terms and Conditions