Technical optimization of oral gavage technique.

Slides:

Advertisements

Similar presentations

Mitochondria localize to both the front and the rear of neutrophils.

Advertisements

Potentiated Hsp104 variants rescue toxicity of ALS-linked FUS mutants.

Postural, morphological and swim defects in Mn-exposed zebrafish larvae. Postural, morphological and swim defects in Mn-exposed zebrafish larvae. (A) Scheme.

Nat. Rev. Gastroenterol. Hepatol. doi: /nrgastro

Retinoic acid sentinels in live embryos and responding to exogenous RA in half-embryo cultures. Retinoic acid sentinels in live embryos and responding.

Fig. 1. Representative images of the four cell lines using fluorescence microscopy. Representative images of the four cell lines using fluorescence microscopy.

Azithromycin treatment eliminates NTHi infection.

Representative illustrations of a sagittal lung section stained with H&E in a control and a CS-exposed mouse after 6 months. Representative illustrations.

BTN1 mRNA level in response to extracellular pH determined by comparative RT2PCR. BTN1mRNA level in response to extracellular pH determined by comparative.

Trends in incidence rates of reported work related injuries and illnesses in jobs with and without exposure, by exposure category. Trends in incidence.

Total and differential cell counting in the broncho-alveolar lavage of mice exposed to CS or air during 3 or 6 months. Total and differential cell counting.

Representative histological images of aorta from WT, ApoEm/m and ApoE−/− pigs. Representative histological images of aorta from WT, ApoEm/m and ApoE−/−

OA and related amines modulate motility and length of schistosomula.

OCT and IOP analysis of 2-3-month-old AP-2β NCC KO mutants.

Crotonate increases histone crotonylation in mouse kidney.

Effect of OLHHA on the protein expression of relevant enzymes and factors related to lipid metabolism in the liver of obese Zucker rats. Effect of OLHHA.

TP53 western blot of primary cultured tail cells from both wild-type (WT) and Tp53Δ11/Δ11 (−/−) rats. TP53 western blot of primary cultured tail cells.

Enterocytes fail to migrate consistently towards the villus tip in live APCMin/+ tissue. Enterocytes fail to migrate consistently towards the villus tip.

Scanning EM shows cilia abnormalities in the neural tube.

Survival and telomere length of G2tert−/− zebrafish in a p53−/− background. Survival and telomere length of G2tert−/− zebrafish in a p53−/− background.

Pie charts showing the percentage of genes for selected enriched biological functions and pathways at different time points after hyperthermic seizures.

Behavioral test parameters.

Fluorescently labeled endocytic cargos enable imaging of caveolar endocytosis in the intact zebrafish intestine. Fluorescently labeled endocytic cargos.

Obesity affected the metabolic response to stroke.

Expression data confirm and extend existing knowledge

Factors affecting standardization of rodent impact TBI models.

Dose-responsiveness and specificity of retinoic acid signaling sentinels. Dose-responsiveness and specificity of retinoic acid signaling sentinels. (A)

tert mutant zebrafish can be bred for only one generation.

Fig. 5. GFP fluorescence colocalization of Gcn5.

Example fluorescence microscopy traces of fluorescein uptake in the presence and absence of a representative OAT inhibitor, methotrexate. Example fluorescence.

Effects of doxorubicin and epirubicin on proliferation and apoptosis in the hyperplastic livers of transgenic zebrafish larvae. Effects of doxorubicin.

Treatment of HCMT-CMs with bisoprolol.

Performance of Drosophila on diet supplemented with B vitamins.

Tamoxifen injections induce DNA damage response signaling in αMHC-MerCreMer mice.αMHC-MerCreMer+/+ mice received an injection of 60 μg/g body weight of.

G418 causes UPF1 to localize to cytoplasmic foci containing NMD substrates but not the P-body marker DCP1a. 6CFSMEo- cells were transfected with constructs.

Elevated expression of vegfaa in hemangiosarcoma.

The effect of TDP-43A315T expression on dendrite spine development.

Common methods to induce acute or chronic OM in mouse models.

The mutants exhibit congenital hypothyroidism.

Distribution and extent of expression.

Comparison ofMyc-induced zebrafish liver tumors with different stages of human HCC and seven mouse HCC models. Comparison ofMyc-induced zebrafish liver.

Functional classification and visualization of differentially expressed genes. Functional classification and visualization of differentially expressed.

Armadillos are the only natural host of leprosy, aside from humans.

The genomic distribution of essential and non-essential mouse genes, separated into known and predicted essentiality. The genomic distribution of essential.

Characterization of zebrafish muscle wasting in krasG12V-induced HCC zebrafish. 4-month-old male adult krasG12V and WT zebrafish were treated with dox.

TWEAK increases histone crotonylation in kidney tubular cells.

Ang-1, ESE-1 and Tie-2 expression levels during teratogen-induced CDH

Homozygous col22a1−/− embryos show increased percentage of hemorrhages when raised at increased temperature. Homozygous col22a1−/− embryos show increased.

Tissue ATP levels measured in the liver, muscle, kidney and pancreas of CDs/y and CDr/y on RD or DD. *P

Impact of low-level galactose exposure on acute and long-term outcomes in GALT-null Drosophila. Impact of low-level galactose exposure on acute and long-term.

SMN1 and SMN2 contribute to spinal muscular atrophy (SMA).

Systemic infection in Drosophila larvae by septic injury with a fine tungsten needle. Systemic infection in Drosophila larvae by septic injury with a fine.

Food-additive exposure increases permeability in a human gut cell co-culture model. Food-additive exposure increases permeability in a human gut cell co-culture.

Surgical technique for orthotopic PDAC tumor injection.

Food-additive exposure reduces adult fly survival under some feeding conditions. Food-additive exposure reduces adult fly survival under some feeding conditions.

Anti-epileptic drugs with Na+ channel blocking activity improve QTc in individuals with Rett syndrome. Anti-epileptic drugs with Na+ channel blocking activity.

Guinea pigs fed HFHC diet develop impaired glucose tolerance and suppressed response to insulin. Guinea pigs fed HFHC diet develop impaired glucose tolerance.

Angiotensin-(1-7) activates the IGF-1/Akt signalling pathway during muscle disuse through the Mas receptor. Angiotensin-(1-7) activates the IGF-1/Akt signalling.

Examples of tumors represented in Tp53Δ11/Δ11 and Tp53Δ11/+ rats.

HFHC/STZ guinea pigs develop insulin-independent diabetes that is responsive to oral antihyperglycemic therapy. HFHC/STZ guinea pigs develop insulin-independent.

Peroxisome speeds were slower in patient and control cells.

Time-lapse imaging of neutrophil migration in vivo.

Fig. 3. Mean force and velocity during jumping

Table 1. Measurement of ring diameters of proteins localizing in ring-like patterns around centrioles.Consideration of the size of IgG (about 8 nm) raises.

Fig. 3. Changes in the total EPS/Chl a ratio and bend interval of trichomes before and after the removal of polysaccharide from the BG11-cultured N. flagelliforme.

Effect of siRNA transfection on colony formation by Renca cells.

Generation of a genetic model of chronic β-cell inflammation in zebrafish. Generation of a genetic model of chronic β-cell inflammation in zebrafish. (A)

Hif-1α stabilisation reduces bacterial burden in zebrafish embryos.

Graphical representation of pain levels versus time postinjury, annotated across control, persistently hypersensitive genotypes and diabetic conditions,

Functional and molecular regeneration in mdx muscle with matrix treatment. Functional and molecular regeneration in mdx muscle with matrix treatment. (A,B)

Presentation transcript:

Technical optimization of oral gavage technique. Technical optimization of oral gavage technique. (A) The gavage apparatus was constructed from a 10 µl luer-tip Hamilton syringe with a 22 G needle and 22 G soft-tip catheter tubing. (B) A representative zebrafish was anesthetized using a MS-222/isoflurane combination anesthetic and immobilized vertically in a damp sponge with a slit. (C) A representative zebrafish was gavaged with 3 μl fluorescein isothiocyanate-dextran (FITC-dextran) to visualize potential regurgitation via fluorescence microscopy. (D) Zebrafish were gavaged with Phenol Red solution to visualize potential regurgitation. Data represented as mean±s.d. of two replicates. (E) Survival curve for extended exposure to anesthetic solutions. Data represented as mean±s.d. of three replicates. (F) Survival curve for long-term daily exposure to anesthetic solution. Data represented as mean±s.d. of three replicates. Michelle Dang et al. Dis. Model. Mech. 2016;9:811-820 © 2016. Published by The Company of Biologists Ltd