Immunhistochemistry by Means of Widely Agreed-Upon Markers (Cytokeratins 5/6 and 7, p63, Thyroid Transcription Factor-1, and Vimentin) on Small Biopsies.

Slides:

Advertisements

Similar presentations

Lung Adenocarcinoma with Ipsilateral Breast Metastasis: A Simple Coincidence? Hsu-Ching Huang, MD, Jen-Fan Hang, MD, Mei-Han Wu, MD, Teh-Ying Chou, MD,

Advertisements

A Prognostic Scoring Model for the Utility of Induction Chemotherapy Prior to Neoadjuvant Chemoradiotherapy in Esophageal Cancer Mian Xi, MD, Zhongxing.

Primary Signet-Ring Carcinoma (SRC) of the Lung: A Population-Based Epidemiologic Study of 262 Cases with Comparison to Adenocarcinoma of the Lung Sai-Hong.

Carcinoma NOS is a Common Histologic Diagnosis and is Increasing in Proportion Among Non-small Cell Lung Cancer Histologies Sai-Hong Ignatius Ou, MD,

Synergistic Activation upon MET and ALK Coamplification Sustains Targeted Therapy in Sarcomatoid Carcinoma, a Deadly Subtype of Lung Cancer Giuseppe.

A Japanese Lung Cancer Registry Study: Prognosis of 13,010 Resected Lung Cancers Hisao Asamura, MD, Tomoyuki Goya, MD, Yoshihiko Koshiishi, MD, Yasunori.

SC17.02 Lung Cancer in China: Challenges and Perspectives

ΔNp63 (p40) and Thyroid Transcription Factor-1 Immunoreactivity on Small Biopsies or Cellblocks for Typing Non-small Cell Lung Cancer: A Novel Two-Hit,

Difficulties encountered managing nodules detected during a computed tomography lung cancer screening program Giulia Veronesi, MD, Massimo Bellomi, MD,

P63 and p73 Isoform Expression in Non-small Cell Lung Cancer and Corresponding Morphological Normal Lung Tissue Marco Lo Iacono, PhD, Valentina Monica,

Malignant Solitary Fibrous Tumors of the Pleura: Retrospective Review of a Multicenter Series Filippo Lococo, MD, Alfredo Cesario, MD, Giuseppe Cardillo,

Gene Expression Profiling of Large Cell Lung Cancer Links Transcriptional Phenotypes to the New Histological WHO 2015 Classification Anna Karlsson, MSc,

APLCC ORAL ABSTRACT SESSIONS - MONDAY, NOVEMBER 26

Results of Surgical Treatment for Patients with Small Cell Lung Cancer

Detection of Lung Cancer through Metabolic Changes Measured in Blood Plasma Evelyne Louis, PhD, Peter Adriaensens, PhD, Wanda Guedens, PhD, Theophile.

Association of Epidermal Growth Factor Receptor Activating Mutations with Low ERCC1 Gene Expression in Non-small Cell Lung Cancer David R. Gandara, MD,

Yih-Leong Chang, MD, Chen-Tu Wu, MD, Yung-Chie Lee, MD, PhD

Ellen M. Schultz, MS, Gerard A. Silvestri, MD, MS, Michael K

KRAS Mutation Analysis Helps to Differentiate Between Pulmonary Metastasis from Colon Adenocarcinoma In Situ and Primary Lung Adenocarcinoma Taylor M.

Gene Expression Profiling of Large Cell Lung Cancer Links Transcriptional Phenotypes to the New Histological WHO 2015 Classification Anna Karlsson, MSc,

Vamsidhar Velcheti, MD, David L. Rimm, MD, PhD, Kurt A

The International Association for the Study of Lung Cancer International Staging Project on Lung Cancer Peter Goldstraw, MB, FRCS, John J. Crowley, PhD

Samuel J. Wang, MD, PhD, C David Fuller, MD, Charles R. Thomas, MD

Expression profiling of non–small cell lung carcinoma identifies metastatic genotypes based on lymph node tumor burden Chuong D. Hoang, MD, Jonathan.

Critical Appraisal of Programmed Death Ligand 1 Reflex Diagnostic Testing: Current Standards and Future Opportunities Matthew P. Humphries, PhD, Stephen.

Kelsie L. Thu, BSc, Raj Chari, PhD, William W

Prognostic role of lymph node involvement in lung metastasectomy

Comprehensive Computational Pathological Image Analysis Predicts Lung Cancer Prognosis Xin Luo, MD, Xiao Zang, BS, Lin Yang, MD, Junzhou Huang, PhD,

Detection of Lung Cancer by Automated Sputum Cytometry

Histologic Transformation in NSCLC with PD-1 therapy

Giant Alveolar Adenoma Causing Severe Dyspnoea

Lung Cancer and Prognosis in Taiwan: A Population-Based Cancer Registry Bing-Yen Wang, MD, Jing-Yang Huang, Ching-Yuan Cheng, MD, Ching-Hsiung Lin, MD,

Intratumoral Lymphatic Vessel Involvement is an Invasive Indicator of Completely Resected Pathologic Stage I Non-small Cell Lung Cancer Masahiko Harada,

Case-Control Study: Smoking History Affects the Production of Tumor Antigen–Specific Antibodies NY-ESO-1 in Patients with Lung Cancer in Comparison with.

A 10-Year Single-Center Experience on 708 Lung Metastasectomies: The Evidence of the “International Registry of Lung Metastases” Monica Casiraghi, MD,

Challenging Lung Carcinoma with Coexistent ΔNp63/p40 and Thyroid Transcription Factor-1 Labeling Within the Same Individual Tumor Cells Giuseppe Pelosi,

MiR-205 and MiR-375 MicroRNA Assays to Distinguish Squamous Cell Carcinoma from Adenocarcinoma in Lung Cancer Biopsies Santosh Patnaik, MD, PhD, Reema.

Tumor-Infiltrating Foxp3+ Regulatory T Cells are Correlated with Cyclooxygenase-2 Expression and are Associated with Recurrence in Resected Non-small.

PD-L1 Expression Heterogeneity in Non–Small Cell Lung Cancer: Defining Criteria for Harmonization between Biopsy Specimens and Whole Sections Enrico.

Japanese Lung Cancer Registry Study: First Prospective Enrollment of a Large Number of Surgical and Nonsurgical Cases in 2002 Noriyoshi Sawabata, MD,

Synchronous Primary Lung Cancer, Breast Cancer Recurrence, and Mediastinal Silicon- Induced Lymphadenitis Francesco Petrella, MD, Gian Carlo Pruneri,

Survival Differences by Gender for Resected Non-small Cell Lung Cancer: A Retrospective Analysis of 12,509 Cases in a Japanese Lung Cancer Registry Study

Prognostic Impact of Node Involvement Pattern in Pulmonary pN1 Squamous Cell Carcinoma Patients Masayuki Nakao, MD, Junji Yoshida, MD, PhD, Genichiro.

Analysis of Tumor Markers in the Cytological Fluid Obtained from Computed Tomography-Guided Needle Aspiration Biopsy for the Diagnosis of Non-small Cell.

ALK Translocation in Non-small Cell Lung Cancer with Adenocarcinoma and Squamous Cell Carcinoma Markers Samuel J. Klempner, MD, David W. Cohen, MD, Daniel.

Can a Thoracic Surgeon Identify Lymph Node Metastases during Surgery Based on Their Size?: Analysis of 844 Metastatic and 10,462 Nonmetastatic Lymph Nodes

The Impact of the Fourth Edition of the WHO Classification of Lung Tumours on Histological Classification of Resected Pulmonary NSCCs Patrick Micke,

An Unmet Need in the WHO 2015 Biopsy Classification: Poorly Differentiated NSCCs with Positive Neuroendocrine Markers Jules L. Derks, MD Journal of.

Use of MicroRNA Expression Levels to Predict Outcomes in Resected Stage I Non- small Cell Lung Cancer Eric Duncavage, MD, Boone Goodgame, MD, Ananth Sezhiyan,

Osimertinib for Epidermal Growth Factor Receptor Mutation–Positive Lung Adenocarcinoma That Transformed to T790M-Positive Squamous Cell Carcinoma Naoyuki.

Impact of Non–Small-Cell Lung Cancer-Not Otherwise Specified Immunophenotyping on Treatment Outcome Luisella Righi, MD, PhD, Tiziana Vavalà, MD, Ida.

“Salvage” Surgery for Primary Mediastinal Malignancies: Is it Worthwhile? Francesco Petrella, MD, Francesco Leo, MD, PhD, Giulia Veronesi, MD, Piergiorgio.

Carlie S. Sigel, MD, Andre L. Moreira, MD, PhD, William D

Trends in Stage Distribution for Patients with Non-small Cell Lung Cancer: A National Cancer Database Survey Daniel Morgensztern, MD, Shean Huey Ng,

Primary Lung Cancer Presenting with Gastrointestinal Tract Involvement: Clinicopathologic and Immunohistochemical Features in a Series of 18 Consecutive.

Associations between TS, TTF-1, FR-α, FPGS, and Overall Survival in Patients with Advanced Non–Small-Cell Lung Cancer Receiving Pemetrexed Plus Carboplatin.

Adjuvant Surgery after Carboplatin and VP16 in Resectable Small Cell Lung Cancer Giulia Veronesi, MD, Paolo Scanagatta, MD, Francesco Leo, MD, Tommaso.

A Prognostic Scoring Model for the Utility of Induction Chemotherapy Prior to Neoadjuvant Chemoradiotherapy in Esophageal Cancer Mian Xi, MD, Zhongxing.

Combined Small Cell Carcinoma of the Lung: Is It a Single Entity?

Genetic Changes in Squamous Cell Lung Cancer: A Review

Carcinoma of the esophagus: Prognostic significance of histologic type

EGFR Mutations in Squamous Cell Lung Cancer in Never-Smokers

Thymidylate Synthase Protein Expression by IHC and Gene Copy Number by SISH Correlate and Show Great Variability in Non–Small Cell Lung Cancer Murry.

Dissecting Pulmonary Large-Cell Carcinoma by Targeted Next Generation Sequencing of Several Cancer Genes Pushes Genotypic-Phenotypic Correlations to Emerge

Four-arm robotic lobectomy for the treatment of early-stage lung cancer Giulia Veronesi, MD, Domenico Galetta, MD, Patrick Maisonneuve, DipEng, Franca.

Superior vena cava replacement for lung cancer using a heterologous (bovine) prosthesis: Preliminary results Lorenzo Spaggiari, MD, PhD, Domenico Galetta,

Time Trends of Surgical Outcome in Patients with Non-small Cell Lung Cancer Takeshi Hanagiri, MD, PhD, Tetsuro Baba, MD, PhD, Tetsuya So, MD, PhD, Manabu.

Proteomic Profiling Identifies Pathways Dysregulated in Non-small Cell Lung Cancer and an Inverse Association of AMPK and Adhesion Pathways with Recurrence

Mark A. Socinski, MD Journal of Thoracic Oncology

MiR-1254 and miR-574-5p: Serum-Based microRNA Biomarkers for Early-Stage Non- small Cell Lung Cancer Kristen M. Foss, BS, Chao Sima, PhD, Donatella Ugolini,

Presentation transcript:

Immunhistochemistry by Means of Widely Agreed-Upon Markers (Cytokeratins 5/6 and 7, p63, Thyroid Transcription Factor-1, and Vimentin) on Small Biopsies of Non-small Cell Lung Cancer Effectively Parallels the Corresponding Profiling and Eventual Diagnoses on Surgical Specimens Giuseppe Pelosi, MD, MIAC, Giulio Rossi, MD, Fabrizio Bianchi, DSc, PhD, Patrick Maisonneuve, Eng, Domenico Galetta, MD, Angelica Sonzogni, MD, Giulia Veronesi, MD, Lorenzo Spaggiari, MD, Mauro Papotti, MD, Mattia Barbareschi, MD, Paolo Graziano, MD, Andrea Decensi, MD, Alberto Cavazza, MD, Giuseppe Viale, MD, FRCPath Journal of Thoracic Oncology Volume 6, Issue 6, Pages 1039-1049 (June 2011) DOI: 10.1097/JTO.0b013e318211dd16 Copyright © 2011 International Association for the Study of Lung Cancer Terms and Conditions

FIGURE 1 Hierarchic unsupervised (A, C) and supervised (B, D) clustering analysis on both surgical specimens (SS in diagrams A and B) and biopsies (BS in diagrams C and D) across the entire tumor series under evaluation. A nonrandom distribution of the relevant markers was shown in both SS (A) and BS (C). Tumor clustering for conventional histology defined consistent profiles to occur for the main tumor types in both SS (B) and BS (D). In particular, CK7 and thyroid transcription factor-1 [TTF1] highlighted adenocarcinoma (AC), CK5/6 and p63 squamous cell carcinoma (SCC), and vimentin sarcomatoid carcinoma (SC) (other included two cases of adenosquamous carcinoma and a case of yolk sac tumor). Journal of Thoracic Oncology 2011 6, 1039-1049DOI: (10.1097/JTO.0b013e318211dd16) Copyright © 2011 International Association for the Study of Lung Cancer Terms and Conditions

FIGURE 2 Two-dimensional diagrams reporting the pairwise comparison for each marker between any two samples according to unsupervised (A) and supervised (B) hierarchical clustering analyses. High coefficients of correlation (C) were found for each marker between surgical specimens (marked as SS) and biopsies (marked as BS) (AC: adenocarcinoma; SCC: squamous cell carcinoma; SC: sarcomatoid carcinoma; other: two adenosquamous carcinomas and one yolk sac tumor). Journal of Thoracic Oncology 2011 6, 1039-1049DOI: (10.1097/JTO.0b013e318211dd16) Copyright © 2011 International Association for the Study of Lung Cancer Terms and Conditions

FIGURE 3 Mosaic plot diagrams (the more surface of varying colored rectangles, the more amount of cases in the different tumor subsets) confirmed a close and distinct relationship of the clusters with the diverse tumor categories in surgical specimens (A) and biopsies (B). AC, adenocarcinoma; SCC, squamous cell carcinoma; SC, sarcomatoid carcinoma (the two cases of adenosquamous carcinoma and the case of yolk sac tumor were excluded from this analysis). Journal of Thoracic Oncology 2011 6, 1039-1049DOI: (10.1097/JTO.0b013e318211dd16) Copyright © 2011 International Association for the Study of Lung Cancer Terms and Conditions

FIGURE 4 Area under the curve (AUC)-receiver operating characteristic (ROC) analysis curves for the different markers are shown in both surgical specimens (A) and small biopsies (B). The high values of the AUC in both types of material testified the high performance of IHC to correctly classify squamous cell carcinoma (p63+ and CK5/6+), adenocarcinoma (CK7+ and thyroid transcription factor-1 [TTF1]+), and sarcomatoid carcinoma (vimentin+). The two cases of adenosquamous carcinoma and the case of yolk sac tumor were excluded from the analysis for the sake of simplicity. Journal of Thoracic Oncology 2011 6, 1039-1049DOI: (10.1097/JTO.0b013e318211dd16) Copyright © 2011 International Association for the Study of Lung Cancer Terms and Conditions

FIGURE 5 Simple rules one should follow for the correct subclassification of NSCLC in biopsy samples when using a five-hit, NSCLC-oriented antibody panel approach with thyroid transcription factor-1 (TTF1), CK7, CK5/6, p63, and vimentin. Journal of Thoracic Oncology 2011 6, 1039-1049DOI: (10.1097/JTO.0b013e318211dd16) Copyright © 2011 International Association for the Study of Lung Cancer Terms and Conditions