Impact of endothelial cell seeding on long-term patency and subendothelial proliferation in a small-caliber highly porous polytetrafluoroethylene graft

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Presentation transcript:

Impact of endothelial cell seeding on long-term patency and subendothelial proliferation in a small-caliber highly porous polytetrafluoroethylene graft E.Charles Douville, M.D., Richard F. Kempczinski, M.D., Louis K. Birinyi, M.D., Georges R. Ramalanjaona, M.D. Journal of Vascular Surgery Volume 5, Issue 4, Pages 544-550 (April 1987) DOI: 10.1016/0741-5214(87)90218-7 Copyright © 1987 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

Fig. 1 Scanning electron micrograph of cut edge of a seeded PTFE graft (GF) at 52 weeks after implantation demonstrates an intact endothelial layer (EC). Note orifice of a luminal capillary (arrow) and a uniform 80 to 90 μm thick subendothelial matrix (SE). (Original magnification ×160.) Journal of Vascular Surgery 1987 5, 544-550DOI: (10.1016/0741-5214(87)90218-7) Copyright © 1987 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

Fig. 2 Light photomicrograph of a seeded graft recovered 52 weeks after implantation demonstrates capillaries, fibrous ingrowth, and macrophages throughout graft wall. (Milligan's trichrome stain; original magnification ×25.) Journal of Vascular Surgery 1987 5, 544-550DOI: (10.1016/0741-5214(87)90218-7) Copyright © 1987 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

Fig. 3 High-power, light photomicrograph of a seeded graft at 1 year demonstrates endothelial cell monolayer subtended by a cellular layer of smooth muscle—like cells beneath which there is a thicker, but less cellular, layer of fibroblasts and collagen. (Hematoxylin-eosin stain; original magnification ×200.) Journal of Vascular Surgery 1987 5, 544-550DOI: (10.1016/0741-5214(87)90218-7) Copyright © 1987 Society for Vascular Surgery and North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions