Validation of novel CARM1 substrates.

Slides:

Advertisements

Similar presentations

Flag-c-Myc HA-REG  Flag-c-Myc WT T58/S62A HA-REG  EGFP C Fig. S1 A B Supplementary Figure S1. Measure the degradation of c-Myc by REG . (A)

Advertisements

Constitutive NF-κB activation by the t(11;18)(q21;q21) product in MALT lymphoma is linked to deregulated ubiquitin ligase activity Honglin Zhou, Ming-Qing.

Evidence for subcomplexes in the Fanconi anemia pathway

A novel SHP-1/Grb2–dependent mechanism of negative regulation of cytokine-receptor signaling: contribution of SHP-1 C-terminal tyrosines in cytokine signaling.

Phosphorylation of serine 73 targets SREBP-1c for ubiquitination and proteasomal degradation Phosphorylation of serine 73 targets SREBP-1c for ubiquitination.

The N‐terminus and SH2 domain of SOCS‐1 contribute to inhibition of JAK1 and JAK2 kinase activity. The N‐terminus and SH2 domain of SOCS‐1 contribute to.

AO domain of LSD1 is responsible for its interaction with Snail1.

PGAM5 dephosphorylates ASK1.

NG2-CreERT2 postnatal day 31

Nore1 enhances interaction between HIPK1 and Mdm2.

MEL23 and MEL24 inhibit Mdm2 and p53 ubiquitination in cells.

Ubiquitination of Drp1 by MARCH‐V.

Pirh2 promotes p73 ubiquitination in vivo.

Volume 28, Issue 3, Pages (September 2015)

Volume 23, Issue 1, Pages (July 2006)

HUWE1 regulates ubiquitination of Shoc2 and RAF-1.

Donghang Cheng, Jocelyn Côté, Salam Shaaban, Mark T. Bedford

Supplement Figure 2 HEK 293 cells

SUMO Promotes HDAC-Mediated Transcriptional Repression

MyD88: An Adapter That Recruits IRAK to the IL-1 Receptor Complex

MED12 interacts with TDRD3 in a CARM1-dependent fashion.

SYCE2 directly binds to the chromoshadow domain of HP1α.

Volume 93, Issue 5, Pages (May 1998)

Volume 18, Issue 5, Pages (January 2017)

Regulation of ChIP-seq target genes by MED12 methylation.

The mutant ZIP13 protein is degraded through a VCP‐dependent mechanism Identification of VCP/Cdc48/p97 as a ZIP13‐associating protein. The mutant ZIP13.

MED12 association with ncRNA-a is disrupted by loss of CARM1 and TDRD3

Expression of SYCE2 inhibits the interaction of HP1α with H3K9me3.

Volume 15, Issue 4, Pages (August 2004)

Fig. 3. HECTD2 contains a naturally occurring polymorphism at A19, which mislocalizes to the cytosol. HECTD2 contains a naturally occurring polymorphism.

Ubqln1 interacts with Ubqln4.

BRCA1 Is a Histone-H2A-Specific Ubiquitin Ligase

Validation of FGFR1 and PLXDC2 as BACE2 substrates in COS-1 overexpression system. Validation of FGFR1 and PLXDC2 as BACE2 substrates in COS-1 overexpression.

ONC201 activates the ATF4 pathway through the eIF2α kinases HRI and PKR. ONC201 activates the ATF4 pathway through the eIF2α kinases HRI and PKR. (A) Western.

Expression of the mutants of HP1α and the mutant of SYCE2 in MCF7 cells or RPE cells. Expression of the mutants of HP1α and the mutant of SYCE2 in MCF7.

TRAF4 is required for EGFR activation in response to EGF stimulation.

Changes in mutation rate or protein abundance are not observed in HATs when comparing rho+ to rho0 cells. Changes in mutation rate or protein abundance.

C-terminal JM-B of EGFR is required for EGF-induced recruitment of TRAF4 to EGFR. (A) The HSQC spectra of 100 μM 15N-labeled TRAF4-TRAF in the absence.

Shrestha Ghosh, Baohua Liu, Yi Wang, Quan Hao, Zhongjun Zhou

TGF-β stimulates SMAD7 expression via FOXO3.

Fig. 5. Expression of either dFMRP or human FMRP does not induce eIF2α phosphorylation.(A) Analysis of eIF2α phosphorylation upon dFMRP expression. Expression.

The interaction of SYCE2 and HP1α is not modified in response to exogenous DNA damage induced by x-ray irradiation. The interaction of SYCE2 and HP1α is.

HSP90β interacts with HNF4A to regulate protein half-life.

Ectopic expression of wt-DAO, but not the inactive mutant, promotes senescence. Ectopic expression of wt-DAO, but not the inactive mutant, promotes senescence.

Figure 4 DNM1 mutations affect protein levels and self-dimerization (A) HeLa cells were transfected with green fluorescent protein (GFP)-tagged mutant.

Validation of DNMT3A splice variant expression.

JAK3A572V mutation causes constitutive JAK3 activity and IL-2–independent proliferation of NKTCL cells. JAK3A572V mutation causes constitutive JAK3 activity.

14-3-3γ phosphorylated at S59 by Lats2 in response to UV damage.

SYCE2 potentiates the steady-state ATM activity.

Fig. 4. Tetracycline-regulated expression of ClC-5 in the HEK293 cells stably expressing gastric H+,K+-ATPase.(A) Alignments of rat ClC-5, human ClC-5,

Effect of ERα Ub-binding ability on E2-induced receptor transcriptional activity. Effect of ERα Ub-binding ability on E2-induced receptor transcriptional.

MEL23 and MEL24 inhibit Mdm2 and p53 ubiquitination through the Mdm2-MdmX hetero-complex. MEL23 and MEL24 inhibit Mdm2 and p53 ubiquitination through the.

Volume 16, Issue 5, Pages (May 2009)

Key functional sites of SPINDLIN1 could be phosphorylated by Aurora-A.

Constitutive NF-κB activation by the t(11;18)(q21;q21) product in MALT lymphoma is linked to deregulated ubiquitin ligase activity Honglin Zhou, Ming-Qing.

Osmotic Stress Affects S6K1 Kinase Activity. (A) Leaves of tobacco (N

Fig. 3 C9ORF72 interacts with SMCR8 in a DENN domain–dependent manner.

Ectopic expression of CA RSK1 mutant protects melanoma cells from PD98059-mediated apoptosis. Ectopic expression of CA RSK1 mutant protects melanoma cells.

Curcumin suppresses the expression of antiapoptotic proteins in multiple myeloma cells. Curcumin suppresses the expression of antiapoptotic proteins in.

Volume 129, Issue 5, Pages (June 2007)

Phosphorylation of the pro-apoptotic protein BAD on serine 155, a novel site, contributes to cell survival K. Virdee, P.A. Parone, A.M. Tolkovsky Current.

PKCζ is tyrosine phosphorylated by EGF and contributes to EGF-induced activation of ERK in Mef cells. PKCζ is tyrosine phosphorylated by EGF and contributes.

Pirh2 represses p73-dependent transactivation.

c-IAP1 Cooperates with Myc by Acting as a Ubiquitin Ligase for Mad1

Fig. 6 The C9ORF72/SMCR8 complex regulates ULK1.

The interaction of PALB2 with BRCA1 is required for the assembly of PALB2, BRCA2, and RAD51 nuclear foci. The interaction of PALB2 with BRCA1 is required.

Association of TCTP with Pim-3 in human pancreatic cancer cell lines.

Stable Cx43 expression in infected Cx43−/− cells and phosphorylation of Cx43. Stable Cx43 expression in infected Cx43−/− cells and phosphorylation of Cx43.

TCTP enhances the protein stability of Pim-3 by blocking the ubiquitin–proteasome degradation of Pim-3. TCTP enhances the protein stability of Pim-3 by.

Expression of MtrE by wild-type and mtr120 mutant strains.

Presentation transcript:

Validation of novel CARM1 substrates. Validation of novel CARM1 substrates. (A, E, F) Whole cell lysates from CARM1 WT (+/+) and KO (−/−) MEFs were immunoprecipitated with antibodies against MED12, SRC-1, or SRC-3 proteins, and the eluted samples were subjected to Western blotting with αADMACARM1 and αH3R17me2a antibodies. αMED12, αSRC-1, and αSRC-3 blots (bottom panels in A, E, F) demonstrate equal expression of these proteins in CARM1 WT (+/+) and KO (−/−) MEFs. (B, C) MCF-7-Tet-on-shCARM1 cells were untreated or treated with doxycycline (1 μg/ml) for 8 d and transiently transfected with FLAG-KMT2D and -GPS2, separately. Total cell lysates were immunoprecipitated with αFLAG antibody, and the eluted samples were subjected to Western blotting with αADMACARM1 and αFLAG antibodies. (D) HeLa cells were transiently transfected with FLAG control, FLAG-KMT2Da, or FLAG-KMT2Da-R3727K. Total cell lysates were immunoprecipitated with αFLAG antibody, and the eluted samples were subjected to Western analysis with αADMACARM1 and αFLAG antibodies. αFLAG blot of the input samples shows equal expression of the WT and mutant KMT2Da proteins. KMT2Da represents a fragment (3,619–4,285 aa) of the full-length KMT2D protein (NP_003473). Donghang Cheng et al. LSA 2018;1:e201800117 © 2018 Cheng et al.