Rapid identification of fungal pathogens in positive blood cultures using oligonucleotide array hybridization H-C. Hsiue, Y-T. Huang, Y-L. Kuo, C-H. Liao, T-C. Chang, P-R. Hsueh Clinical Microbiology and Infection Volume 16, Issue 5, Pages 493-500 (May 2010) DOI: 10.1111/j.1469-0691.2009.02828.x Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions
Fig. 1 Layout of oligonucleotide probes on the yeast (a) and mould (b) arrays. PC, positive control; NC, negative control; M, position marker. The yeast array contained 108 spots, including 80 spots for identification of 77 species, two positive controls, seven negative controls (tracking dye only), and 19 position markers. The mould array contained 88 spots, 69 spots for identification of 66 species, one positive control, one negative control and 17 position markers. The species corresponding to each probe code is listed in Table 1. Clinical Microbiology and Infection 2010 16, 493-500DOI: (10.1111/j.1469-0691.2009.02828.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions
Fig. 2 Representative hybridization results. All isolates were hybridized to the yeast array except for Penicillium marneffei, which is hybridized to the mould array. Clinical Microbiology and Infection 2010 16, 493-500DOI: (10.1111/j.1469-0691.2009.02828.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions