Carrie C. Lubitz, Sareh Parangi, Tammy M. Holm, M

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Presentation transcript:

Detection of Circulating BRAFV600E in Patients with Papillary Thyroid Carcinoma Carrie C. Lubitz, Sareh Parangi, Tammy M. Holm, M. Jordana Bernasconi, Aislyn P. Schalck, Hyunsuk Suh, Konstantinos P. Economopoulos, Viswanath Gunda, Samuel E. Donovan, Peter M. Sadow, Lori J. Wirth, Ryan J. Sullivan, David J. Panka The Journal of Molecular Diagnostics Volume 18, Issue 1, Pages 100-108 (January 2016) DOI: 10.1016/j.jmoldx.2015.08.003 Copyright © 2016 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 1 A: Schematic of the BRAFV600E assay. B: Standard curve of the BRAF assay used for quantification. The equation representative of the best fit line for the BRAFV600E (lower equation) and the wild-type (WT) BRAF (upper equation) are shown. Experiments were used to calculate the standard deviations of the a375 (BRAFV600E hemizygous cell line) and 786 (BRAF wild-type cell line), respectively. n = 5 and 3 experiments. Panel A was reproduced from Panka et al25 with permission from American Association for Cancer Research. The Journal of Molecular Diagnostics 2016 18, 100-108DOI: (10.1016/j.jmoldx.2015.08.003) Copyright © 2016 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 2 Receiver-operating analysis with each of the 70 circulating BRAFV600E levels as a possible positivity criterion in predicting the gold standard (tissue BRAF testing). AUC, area under the curve. The Journal of Molecular Diagnostics 2016 18, 100-108DOI: (10.1016/j.jmoldx.2015.08.003) Copyright © 2016 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 3 Circulating levels of BRAFV600E by pathologic diagnosis (benign, left; malignant, right) and tissue mutational status. Benign/BRAF− indicates a tumor that was read as either an adenoma or a benign hyperplastic lesion that is also negative for BRAFV600E by conventional tissue testing. Benign/BRAF+ indicates pathologically negative, tissue BRAFV600E+. Asterisk indicates patients with known distant metastatic disease. The Journal of Molecular Diagnostics 2016 18, 100-108DOI: (10.1016/j.jmoldx.2015.08.003) Copyright © 2016 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions