Immotilin, a novel sperm immobilizing protein Mohua Mukherjee, M.Sc., Malabika Datta, Ph.D., Shampa Biswas, M.Sc., Alok K Pal, Ph.D., Dipankar Malakar, M.Sc., Asok K Bhattacharyya, Ph.D, D.Sc., Samir Bhattacharya, Ph.D., Hideshi Kobayashi, Ph.D.  Fertility and Sterility  Volume 79, Pages 1673-1675 (June 2003) DOI: 10.1016/S0015-0282(03)00371-6

FIGURE 1 (A) SDS-PAGE of immotilin: 7.0 μg of the purified protein was loaded on the gel and electrophoresed through a 12% separating gel. (B) Human control sperm and sperm treated with immotilin (7.5 μg protein) were stained with eosin-nigrosin. Immotilin-treated sperm show uptake of the dye. In each case 25–30 million sperm were used. (Magnification: 100×) (C) Release of acrosin and 5′-nucleotidase from human sperm by immotilin: 7.5 μg immotilin was added to human sperm suspension and after 5 minutes of incubation, the amount of acrosin or 5′-nucleotidase released was estimated. Each value is the mean ± SEM of independent incubations from seven individuals. (*P<.001 as compared to control.) (D) Fertilized embryos in the two-cell stage from control inseminated horn (C) and unfertilized oocytes from another uterine horn inseminated in the presence of immotilin (T). (Magnification: 200×.) (E) Presence and absence of implantation sites in the control (C) and immotilin-treated (T) uterine horns. Mukherjee. Sperm immobilizing protein. Fertil Steril 2003. Fertility and Sterility 2003 79, 1673-1675DOI: (10.1016/S0015-0282(03)00371-6)