Expression of transforming growth factor (TGF)-β1, TGF-β2, and TGF-β3 and of type I and II TGF-β receptors during the development of the human fetal ovary  Birte Schilling, M.D., John Yeh, M.D.  Fertility and Sterility  Volume 72, Issue 1, Pages 147-153 (July 1999) DOI: 10.1016/S0015-0282(99)00182-X

FIGURE 1 Results of reverse transcription-polymerase chain reaction (RT-PCR) analysis and subsequent restriction enzyme of transforming growth factor (TGF)-β1, TGF-β2, TGF-β3, receptor I (RI), and receptor II (RII) in human fetal ovarian tissue. (A), RT-PCR digestion analysis results: lane 1, 100–base pair (bp) ladder (M = marker); lanes 2–6, human fetal ovaries at 11, 14, 18, 20, and 22 weeks of gestation; lane 7, placenta (+ = positive control); lane 8, yeast transfer RNA (− = negative control); and lane 9, water (− = negative control). (B), Restriction enzyme digestion to verify the identity of RT-PCR products: lane 1, 100-bp ladder (M = marker); lane 2, restriction enzyme digestion fragments (from a representative 20-week ovary); and lane 3, undigested intact PCR product (20-week ovary). All fetal age groups as well as the placenta (positive control) demonstrated the appropriate products from RT-PCR analysis and from restriction enzyme digestion. Fertility and Sterility 1999 72, 147-153DOI: (10.1016/S0015-0282(99)00182-X)

FIGURE 2 Photomicrographs of transforming growth factor (TGF)-β isoform distribution in human fetal ovaries. The photographs on the left show 11-week fetal ovaries; the photographs on the right show 22-week fetal ovaries. Each slide is representative of at least two different samples in each age group. The samples were paraffin-embedded and incubated with anti–TGF-β1 (A and B), anti–TGF-β2 (C and D), and anti–TGF-β3 (E and F), and were visualized using diaminobenzidine. Thick arrows = oocytes; thin arrows = pregranulosa cells. Magnification, ×400. Fertility and Sterility 1999 72, 147-153DOI: (10.1016/S0015-0282(99)00182-X)

FIGURE 3 Photomicrographs of transforming growth factor (TGF)-β receptor distribution in human fetal ovaries. The photographs on the left show 11-week fetal ovaries; the photographs on the right show 24-week fetal ovaries. Each slide is representative of at least two different samples in each age group. The samples were paraffin-embedded and incubated with anti–TGF-β receptor I (A and B), anti–TGF-β receptor II (C and D), and rabbit immunoglobulin G (negative control; E and F), and were visualized using diaminobenzidine. Thick arrows = oocytes; thin arrows = pregranulosa cells. Magnification, ×400. Fertility and Sterility 1999 72, 147-153DOI: (10.1016/S0015-0282(99)00182-X)